Plasma Permeabilization of Human Excised Full-Thickness Skin by µs- and ns-pulsed DBD

Gelker, Monika; Müller‐Goymann, Christel C.; Viöl, Wolfgang

doi:10.1159/000505195

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…4 ). As CSCs are the key drivers of cancer recurrence, metastasis and drug resistance, we anticipate a long-term success by combining CAP with canonical approaches 58 - 62 besides Atorvastatin such as chemo-, radio- and immune-therapies towards ultimate cancer eradication by dual targeting of CSCs and the bulk tumor cells with reduced adverse effects. In addition, we anticipate the efficacy of CAP in rewiring the abnormal regulatory circuits that favors high cancer stemness be extendable to all cancers with high plasticity especially those lack safe cure.…”

Section: Discussionmentioning

confidence: 99%

Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitination

Dai¹,

Cai²,

Wang³

et al. 2022

Int. J. Biol. Sci.

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Cold atmospheric plasma (CAP) is selective against many cancers with little side effect, yet its molecular mechanism remains unclear. Through whole transcriptome sequencing followed by assays in vitro , in vivo and using clinical samples, we propose CAP as a promising onco-therapy targeting cancer stemness via the AQP3/FOXO1 axis. CAP-generated reactive species penetrated cells via AQP3 and suppressed RPS6KA3, a shared kinase of AQP3 and FOXO1. Reduced AQP3-19Y phosphorylation suppressed SCAF11-mediated AQP3-5K K48-ubiquitination that led to sabotaged FOXO1 stability. Inhibited FOXO1 phosphorylation retarded its regulatory activities in maintaining cancer stemness including ALDH1 and IL6. Enhanced anti-cancer efficacy was observed through combining CAP with Atorvastatin in vitro and in vivo . We propose CAP as a 'selective' onco-therapeutic against cancer stemness, with the AQP3/FOXO1 axis being one molecular mechanism. We report SCAF11 as an E3 ubiquitin ligase of both AQP3 and FOXO1, identify AQP3-5K as an AQP3 K48-ubiquitination site, and emphasize the essential role of AQP3-19Y in this process. We reposition Atorvastatin into the onco-therapeutic portfolio by synergizing it with CAP towards enhanced efficacy. We anticipate the efficacy of CAP in targeting malignancies of high stemness alone or as an adjuvant therapy towards the hope of ultimate cancer cure.

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Section: Discussionmentioning

confidence: 99%

Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitination

Dai¹,

Cai²,

Wang³

et al. 2022

Int. J. Biol. Sci.

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“…This effect had been shown for hydrophilic sodium fluorescein molecule (C 20 H 10 Na 2 O 5 ), which was able to effectively pass through stratum corneum samples derived from the skin tissue (ex vivo). According to Gelker et al [ 46 ], permeabilization was attributed to pore formation induced by local Joule heating. However, this effect is currently not well understood, and, e.g., threshold values for power densities that induce pore formation are not available.…”

Section: Discussionmentioning

confidence: 99%

Topically confined enhancement of cutaneous microcirculation by cold plasma

Borchardt¹,

Helmke²,

Ernst³

et al. 2022

Skin Pharmacol Physiol

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Introduction: We aim to explore potentials and modalities of cold atmospheric pressure plasma (CAP) for the subsequent development of therapies targeting an increased perfusion of the lower leg skin tissue. In this study, we addressed the question whether the microcirculation enhancement is restricted to the tissue in direct contact with plasma or if adjacent tissue might also benefit. Methods: A dielectric barrier discharge (DBD)-generated CAP device exhibiting an electrode area of 27.5 cm² was used to treat the anterior lower leg of ten healthy subjects for 4.5 minutes. Subsequently, hyperspectral imaging (HIS) was performed to measure tempo-spatially resolved characteristics of microcirculation parameters in superficial (up to 1 mm) and deeper (up to 5 mm) skin layers. Results: In the tissue area covered by the plasma electrode, DBD-CAP treatment enhances most of the perfusion parameters. The maximum oxygen saturation (StO2) increase reached 8 %, the near infrared perfusion index (NIR) increases by a maximum of 4 %, and the maximum tissue hemoglobin (THI) increase equaled 14 %. Tissue water index (TWI) was lower in both the control and the plasma group thus not affected by the DBD-CAP treatment. Yet, our study reveals that adjacent tissue is hardly affected by the enhancements in the electrode area and the effects are locally confined. Conclusion: Application of DBD-CAP to the lower leg resulted in enhancement of cutaneous microcirculation that extended 1 h beyond the treatment period with localization to the tissue area in direct contact with the cold plasma. This suggests the possibility of tailoring application schemes for topically confined enhancement of skin microcirculation, e.g. in the treatment of chronic wounds.

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“…In two consecutive studies by Gelker et al., the electrical characteristics of plasma could be tailored to cause differential permeabilization of excised human full-thickness skin ( 120 , 121 ). Both μs-pulsed and ns-pulsed DBD plasma could significantly reduce the transepithelial electrical resistance (TEER).…”

Section: Improving Drug Absorbance Into Skin Using Capmentioning

confidence: 99%

Plasma Dermatology: Skin Therapy Using Cold Atmospheric Plasma

et al. 2022

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Cold atmospheric plasma-based plasma medicine has been expanding the diversity of its specialties. As an emerging branch, plasma dermatology takes advantage of the beneficial complexity of plasma constituents (e.g., reactive oxygen and nitrogen species, UV photons, and electromagnetic emission), technical versatility (e.g., direct irradiation and indirect aqueous treatment), and practical feasibility (e.g., hand-held compact device and clinician-friendly operation). The objective of this comprehensive review is to summarize recent advances in the CAP-dominated skin therapy by broadly covering three aspects. We start with plasma optimisation of intact skin, detailing the effect of CAP on skin lipids, cells, histology, and blood circulation. We then conduct a clinically oriented and thorough dissection of CAP treatment of various skin diseases, focusing on the wound healing, inflammatory disorders, infectious conditions, parasitic infestations, cutaneous malignancies, and alopecia. Finally, we conclude with a brief analysis on the safety aspect of CAP treatment and a proposal on how to mitigate the potential risks. This comprehensive review endeavors to serve as a mini textbook for clinical dermatologists and a practical manual for plasma biotechnologists. Our collective goal is to consolidate plasma dermatology’s lead in modern personalized medicine.

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Plasma Permeabilization of Human Excised Full-Thickness Skin by µs- and ns-pulsed DBD

Cited by 13 publications

References 22 publications

Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitination

Cold atmospheric plasmas target breast cancer stemness via modulating AQP3-19Y mediated AQP3-5K and FOXO1 K48-ubiquitination

Topically confined enhancement of cutaneous microcirculation by cold plasma

Plasma Dermatology: Skin Therapy Using Cold Atmospheric Plasma

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