Plasma plasminogen activator inhibitor-I is associated with plasma leptin irrespective of body mass index, body fat mass, and plasma insulin and metabolic parameters in premenopausal women

Mitrio, V. De; Pergola, Giovanni De; Vettor, Roberto; Marino, Renato; Sciaraffia, M; Pagano, Claudio; Scaraggi, F. A.; Lorenzo, Luigi Di; Giorgino, R

doi:10.1016/s0026-0495(99)90190-7

Cited by 38 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As human adipose tissue is an important site of PAI-1 production 20 -22 and as plasma PAI-1 levels and leptin concentrations are closely correlated 23 we looked at the effect of leptin on the regulation of PAI-1 secretion. Our results did not verify this hypothesis for in vitro differentiated human adipocytes and clearly suggest that leptin does not contribute to the disturbances of the fibrinolytic system characteristic for obesity.…”

Section: Discussionmentioning

confidence: 99%

Effects of leptin on the differentiation and metabolism of human adipocytes

et al. 2001

View full text Add to dashboard Cite

BACKGROUND:Leptin is an adipose protein regulating food intake in the hypothalamus. Animal studies have suggested that leptin also acts in an auto-=paracrine fashion on adipose cell function. OBJECTIVE: The aim of this study was to investigate the effects of leptin on the differentiation and metabolism of cultured human adipocytes. MATERIAL: Adipose tissue from young healthy, lean women (body mass index (BMI) < 27 kg=m 2 ) undergoing elective mammary reduction surgery and young obese individuals (BMI > 40 kg=m 2 ) undergoing laparoscopic gastric banding. METHODS: Human preadipocytes in primary culture were induced to undergo differentiation by defined adipogenic factors. Mature adipocytes were isolated by collagenase digestion and kept in culture suspension. Glycero-3-phosphate dehydrogenase (GPDH) activity was used as a marker of adipose differentiation; glucose uptake, lipolysis and PAI-1 secretion were measured as parameters of fat cell function. RESULTS: Human preadipocytes and adipocytes from lean and obese subjects expressed the long leptin receptor isoform and two of the three short forms as assessed by polymerase chain reaction (PCR). Leptin at a supraphysiological concentration induced a transient increase of GPDH activity, but had no effect on glucose uptake and PAI-1 secretion from human adipocytes. In addition, basal and isoproterenol-stimulated lipolysis as well as the antilipolytic action of insulin in human adipocytes was not significantly affected by leptin exposure. CONCLUSION: In contrast to animal data, the results of our experiments do not demonstrate significant effects of leptin on main metabolic functions of human adipocytes arguing against a local auto-=paracrine action of leptin in human adipose tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of leptin on the differentiation and metabolism of human adipocytes

et al. 2001

View full text Add to dashboard Cite

show abstract

“…20 The fat-free mass (FFM) was calculated by Heitmann's equation 21 and fat mass (FM) was calculated as the difference between body weight and FFM.…”

Section: Methodsmentioning

confidence: 99%

Free testosterone plasma levels are negatively associated with the intima-media thickness of the common carotid artery in overweight and obese glucose-tolerant young adult men

et al. 2003

View full text Add to dashboard Cite

OBJECTIVE:To evaluate the relation between free testosterone (FT) levels and the intima-media thickness of the common carotid artery (IMT-CCA) in overweight and obese glucose-tolerant (NGT) young adult men. DESIGN: Cross-sectional study of FT and IMT-CCA in obese men. SUBJECTS: A total of 127 overweight and obese NGT male individuals, aged 18-45 y. MEASUREMENTS: FT plasma levels; IMT-CCA, as measured by high-resolution B-mode ultrasound imaging; central fat accumulation, as evaluated by waist circumference; body composition, as measured by bioimpedance analysis; insulin resistance, as calculated by homeostatic model assessment (HOMA IR ); systolic and diastolic blood pressure; and fasting concentrations of glucose, insulin, and lipids. RESULTS: IMT-CCA was positively correlated with age, body mass index (BMI), fat mass (FM), waist circumference, and fasting glucose concentrations, and inversely associated with FT levels. After multivariate analysis, IMT-CCA maintained an independent association with BMI, FM, and FT levels. This study indicates that IMT-CCA is negatively associated with FT levels, independent of age, total body fat, central fat accumulation, and fasting glucose concentrations in overweight and obese NGT patients. CONCLUSION: Hypotestosteronemia may accelerate the development of atherosclerosis and increase the risk for CHD in obese men.

show abstract

“…It has been shown that, in dieting obese women, the decrease in PAI-1 during weight loss is more closely related to changes in fat mass than to changes in metabolic variables, suggesting a significant role of adipose tissue in regulating plasma PAI-1 concentrations (107). However, a strong correlation E832 exists between PAI-1 antigen and circulating leptin concentrations irrespective of body mass index, and body fat mass seems to indicate that leptin per se may potentially increase PAI-1 in obese subjects (25,150).…”

Section: Pai-1mentioning

confidence: 96%

The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation

Frühbeck

Gómez-Ambrosi²,

Muruzábal

et al. 2001

American Journal of Physiology-Endocrinology and Metabolism

812

571

View full text Add to dashboard Cite

The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation. Am J Physiol Endocrinol Metab 280: E827-E847, 2001.-The ability to ensure continous availability of energy despite highly variable supplies in the environment is a major determinant of the survival of all species. In higher organisms, including mammals, the capacity to efficiently store excess energy as triglycerides in adipocytes, from which stored energy could be rapidly released for use at other sites, was developed. To orchestrate the processes of energy storage and release, highly integrated systems operating on several physiological levels have evolved. The adipocyte is no longer considered a passive bystander, because fat cells actively secrete many members of the cytokine family, such as leptin, tumor necrosis factor-␣, and interleukin-6, among other cytokine signals, which influence peripheral fuel storage, mobilization, and combustion, as well as energy homeostasis. The existence of a network of adipose tissue signaling pathways, arranged in a hierarchical fashion, constitutes a metabolic repertoire that enables the organism to adapt to a wide range of different metabolic challenges, such as starvation, stress, infection, and short periods of gross energy excess. leptin; tumor necrosis factor-␣; interleukins; obesity; insulin resistance OVERVIEW Unraveling the diverse hormonal and neuroendocrine systems that regulate energy balance and body fat has been a long-standing challenge in biology, with obesity as an increasingly important public health focus (12). Adipose tissue is the body's largest energy reservoir. For example, an adult with 15 kg of body fat has Ͼ460 MJ (110,000 kcal) of lipid fuel stores, which could provide 8.37 MJ (2,000 kcal) daily for ϳ2 mo (75, 129). The primary role of adipocytes is to store triacylglycerol during periods of caloric excess and to mobilize this reserve when expenditure exceeds intake. Mature adipocytes are uniquely equipped to perform these functions. They possess the full complement of enzymes and regulatory proteins needed to carry out both lipolysis and de novo lipogenesis. In fat cells, the regulation of these processes is exquisitely responsive to hormones, cytokines, and other factors that are involved in energy metabolism (87). The ability to carry out these functions is acquired during embryonic development in preparation for the postnatal period, when an adipose energy reserve becomes necessary. Major expansion of the white adipocyte population is delayed until shortly after birth, although preadipocytes first appear late in embryonic life (22).The present review will focus on the evidence for the synthesis and secretion by white adipocytes of endocrine, paracrine, and autocrine signals implicated in energy balance regulation, with special reference to cytokines. The interactions between these adipose tissue-derived mediators and other neuroendocrine pathways will be examined. Furthermore, the metabolic alterations in adipose tissue signaling leading...

show abstract

Plasma plasminogen activator inhibitor-I is associated with plasma leptin irrespective of body mass index, body fat mass, and plasma insulin and metabolic parameters in premenopausal women

Cited by 38 publications

References 41 publications

Effects of leptin on the differentiation and metabolism of human adipocytes

Effects of leptin on the differentiation and metabolism of human adipocytes

Free testosterone plasma levels are negatively associated with the intima-media thickness of the common carotid artery in overweight and obese glucose-tolerant young adult men

The adipocyte: a model for integration of endocrine and metabolic signaling in energy metabolism regulation

Contact Info

Product

Resources

About