Plasma Polymer Coatings to Support Mesenchymal Stem Cell Adhesion, Growth and Differentiation on Variable Stiffness Silicone Elastomers

Colley, Helen; Mishra, Gautam; Scutt, Andrew; McArthur, Sally L.

doi:10.1002/ppap.200900040

Cited by 50 publications

(53 citation statements)

References 36 publications

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“…The presence of surface acid groups increases the number of oxygencontaining species on the surface, which correlates positively with surface wettability, and in most cases cell adhesion. 36,37 Our study showed, as expected, that cell attachment to uncoated silicone substrates was extremely poor, whereas attachment to the more wettable ppAAc-coated substrate was very good (Fig. 5A, B).…”

Section: Discussionsupporting

confidence: 83%

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Walker

Mistry²,

Smith³

et al. 2012

Tissue Engineering Part C: Methods

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Skin has a remarkable capacity for regeneration, but age-and diabetes-related vascular problems lead to chronic non-healing wounds for many thousands of U.K. patients. There is a need for new therapeutic approaches to treat these resistant wounds. Donor mesenchymal stem/stromal cells (MSCs) have been shown to assist cutaneous wound healing by accelerating re-epithelialization. The aim of this work was to devise a low risk and convenient delivery method for transferring these cells to wound beds. Plasma polymerization was used to functionalize the surface of medical-grade silicone with acrylic acid. Cells attached well to these carriers, and culture for up to 3 days on the carriers did not significantly affect their phenotype or ability to support vascular tubule formation. These carriers were then used to transfer MSCs onto human dermis. Cell transfer was confirmed using an MTT assay to assess viable cell numbers and enhanced green fluorescent protein-labeled MSCs to demonstrate that the cells post-transfer attached to the dermis. We conclude that this synthetic carrier membrane is a promising approach for delivery of therapeutic MSCs and opens the way for future studies to evaluate its impact on repairing difficult skin wounds.

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Section: Discussionsupporting

confidence: 83%

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Walker

Mistry²,

Smith³

et al. 2012

Tissue Engineering Part C: Methods

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“…While hASCs grew in clusters and in a rounded shape on untreated silicone, cells grown on plasma-treated silicone exhibited the characteristic fibroblast-like phenotype. Similar effects were reported by Kim et al [20] for human stomach cancer cells grown on oxygen plasma-treated silicone and by Colley et al [21] for mesenchymal stem cells grown on plasma polymer-coated silicone.…”

Section: Discussionsupporting

confidence: 75%

Plasma-Assisted Hydrophilization of Cochlear Implant Electrode Array Surfaces Enables Adhesion of Neurotrophin-Secreting Cells

Schendzielorz

Schmitz²,

Moseke³

et al. 2014

ORL

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Objective: To evaluate plasma treatment for enhancing the biocompatibility of cochlear implant (CI) silicone surfaces, thus allowing colonization with human adipose-derived stem cells (hASCs) that are known to provide neurotrophic support. Methods: Silicone samples and CI electrode arrays were treated with 4 low-pressure plasmas of different characteristics. The hydrophilicity of plasma-treated and control surfaces as well as the adherence and morphology of hASCs were assessed. Finally, the insertion forces of electrode arrays were determined and the colonization potential of the electrode arrays with hASCs were tested. Results: The hydrophilicity of the silicone surfaces was significantly enhanced after plasma treatment, as was the adherence of hASCs. The characteristic morphology of hASCs was observed when grown on plasma-treated but not on untreated silicone surfaces. The insertion forces of plasma-treated electrode arrays were similar to those of untreated arrays, and the colonization of plasma-treated electrode arrays with hASCs was feasible. Conclusion: Plasma treatment of CI electrode arrays enhances their biocompatibility and allows for the colonization with hASCs that are known to produce neurotrophic factors. i 2014 S. Karger AG, Basel

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“…The study of plasma polymer coatings that could potentially be used in biomedical devices is an area of increasing research interest [1][2][3][4][5]. One of the most common classes of thin film treatments employed in biomaterial devices is that of 'low-fouling' or 'stealth' coatings [6].…”

Section: Introductionmentioning

confidence: 99%

An X-ray and neutron reflectometry study of ‘PEG-like’ plasma polymer films

Menzies

Nelson

Shen

et al. 2011

J. R. Soc. Interface.

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Plasma-enhanced chemical vapour-deposited films of di(ethylene glycol) dimethyl ether were analysed by a combination of X-ray photoelectron spectroscopy, atomic force microscopy, quartz crystal microbalance with dissipation monitoring (QCM-D), X-ray and neutron reflectometry (NR). The combination of these techniques enabled a systematic study of the impact of plasma deposition conditions upon resulting film chemistry (empirical formula), mass densities, structure and water solvation, which has been correlated with the films' efficacy against protein fouling. All films were shown to contain substantially less hydrogen than the original monomer and absorb a vast amount of water, which correlated with their mass density profiles. A proportion of the plasma polymer hydrogen atoms were shown to be exchangeable, while QCM-D measurements were inaccurate in detecting associated water in lower power films that contained loosely bound material. The higher protein resistance of the films deposited at a low load power was attributed to its greater chemical and structural similarity to that of poly(ethylene glycol) graft surfaces. These studies demonstrate the utility of using X-ray and NR analysis techniques in furthering the understanding of the chemistry of these films and their interaction with water and proteins.

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Plasma Polymer Coatings to Support Mesenchymal Stem Cell Adhesion, Growth and Differentiation on Variable Stiffness Silicone Elastomers

Cited by 50 publications

References 36 publications

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

Plasma-Assisted Hydrophilization of Cochlear Implant Electrode Array Surfaces Enables Adhesion of Neurotrophin-Secreting Cells

An X-ray and neutron reflectometry study of ‘PEG-like’ plasma polymer films

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