Plasma‐Polymerized Films for Biochip Design

Muguruma, Hitoshi

doi:10.1002/ppap.200900125

Cited by 26 publications

(21 citation statements)

References 74 publications

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“…In the development of, e.g., biomarkers and biosensors or in molecular design, nanoparticles are very often applied [88][89][90]. These may be prepared from organic materials (e.g., polymers) and inorganic materials (e.g., metal nanoparticles).…”

Section: Grafting Of Plasma-treated Polymersmentioning

confidence: 99%

Wettability and Other Surface Properties of Modified Polymers

Kasálková¹,

Slepička²,

Kolská³

et al. 2015

Wetting and Wettability

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Surface wettability is one of the crucial characteristics for determining of a material's use in specific application. Determination of wettability is based on the measurement of the material surface contact angle. Contact angle is the main parameter that characterizes the drop shape on the solid surface and is also one of the directly measurable properties of the phase interface. In this chapter, the wettability and its related properties of pristine and modified polymer foils will be described. The wettability depends on surface roughness and chemical composition. Changes of these parameters can adjust the values of contact angle and, therefore, wettability. In the case of pristine polymer materials, their wettability is unsuitable for a wide range of applications (such as tissue engineering, printing, and coating). Polymer surfaces can easily be modified by, e.g., plasma discharge, whereas the bulk properties remain unchanged. This modification leads to oxidation of the treated layer and creation of new chemical groups that mainly contain oxygen. Immediately after plasma treatment, the values of the contact angles of the modified polymer significantly decrease.In the case of a specific polymer, the strongly hydrophilic surface is created and leads to total spreading of the water drop. Wettability is strongly dependent on time from modification.Wettability plays a key role, e.g., in the development of biomaterials in tissue engineering and regenerative medicine. Biocompatibility tests of the cell adhesion, proliferation and viability are performed in an aqueous medium, and it is necessary to control the surface wettability. Various cell types have different requirements on surface properties, but while maintaining suitable parameters, the optimal value of © 2015 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.water contact angle for cell adhesion is in the interval of 50-70°. In the case of polymers, which have usually higher values of contact angles, the decrease in water contact angle and adjustment of the surface may be accomplished by introducing selected chemical groups (by plasma treatment), chemical compounds (by grafting, i.e., chemical bath deposition), or nanoparticles. In the case of grafting of polyethylene glycol (PEG) on the plasma activated high-density polyethylene was under "properly" selected conditions of modification (molecular weight of PEG, concentration of PEG in solution) prepared layers that positively influenced the cell adhesion. In addition, low concentration of gold nanoparticles increased the number of adhered cells without decreasing cell viability.Whether the surface of the polymeric substrate is attractive for the adhesion and proliferation of cells is determined not only by wettability but also by a range of other surface properties...

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Section: Grafting Of Plasma-treated Polymersmentioning

confidence: 99%

Wettability and Other Surface Properties of Modified Polymers

Kasálková¹,

Slepička²,

Kolská³

et al. 2015

Wetting and Wettability

View full text Add to dashboard Cite

show abstract

“…Nanotechnology is undergoing rapid development, and it is expected to produce innovations in different fields. It plays a crucial role in various biomedical applications, not only in targeted drug delivery but also in molecular imaging, biomarkers, and biosensors [51][52][53][54][55][56]. Nanoparticles can be prepared either from organic materials (e.g., polymers) or from inorganic ones (e.g., metal nanoparticles).…”

Section: Nanoparticle Graftingmentioning

confidence: 99%

Enhancement of Polymer Cytocompatibility by Nanostructuring of Polymer Surface

Slepička

Kasálková

Bačáková

et al. 2012

Journal of Nanomaterials

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Polymers with their advantageous physical, chemical, mechanical, and electrical properties and easy manufacturing are widely used in biology, tissue engineering, and medicine, for example, as prosthetic materials. In some cases the polymer usage may be impeded by low biocompatibility of common synthetic polymers. The biocompatibility can be improved by modification of polymer surface, for example, by plasma discharge, irradiation with ionizing radiation, and sometime subsequent grafting with suitable organic (e.g., amino-acids) or inorganic (e.g., gold nanoparticles) agents. In this way new chemically active structures are created on the polymer surface, and in some cases new surface relief is created. Recent advances in nanotechnology and in characterization of nanostructured objects open the way to development of new polymer-based materials with better bio-properties and higher application potential in biomedicine. Some of recent results obtained in the field are summarized and discussed in this paper.

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“…In the last decades, one of the most critical issues in biosensing devices was the proper surface chemical modification aimed to covalently immobilize a bioreceptor (e.g. a capture antibody) or probe (a single-stranded DNA, ss-DNA), able to selectively bind a target, by matching an antigen or an oligonucleotide sequence (DNA or RNA fragment) of interest for biodiagnostics, respectively [1][2][3]. Polymeric thin coatings, such as polyacrylic acid, polydi(ethylene glycol) monovinyl ether, maleic anhydride, obtained by a plasma-enhanced chemical vapour deposition (PECVD) [4][5][6][7][8], at low-pressure conditions, are quite suitable for the chemical functionalization of a sensing surface [9,10], owing to the following reasons: (i) they do not change the chemical properties of the bulky material of a sensing device so leaving unaffected its working principle; (ii) they are conformal and (iii) do not need particular chemistry exposed to the sensor surface for adhesion; and (iv) they can show different features in terms of density of functional groups, wettability (hydrophilicity/hydrophobicity) and chemical stability by simply varying the process parameters (modulation of the plasma discharge, monomer or mixture of monomers/process gas, reactant vapour partial pressure, etc.…”

Section: Introductionmentioning

confidence: 99%

Ultra-Thin Plasma-Polymerized Functional Coatings for Biosensing: Polyacrylic Acid, Polystyrene and Their Co-Polymer

Rivolo¹,

Castellino²,

Frascella³

et al. 2016

Crystalline and Non-Crystalline Solids

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Recently, many efforts have been done to chemically functionalize sensors surface to achieve selectivity towards diagnostics targets, such as DNA, RNA fragments and protein tumoural biomarkers, through the surface immobilization of the related specific receptor. Especially, some kind of sensors such as microcantilevers (gravimetric sensors) and one-dimensional photonics crystals (optical sensors) able to couple Bloch surface waves are very sensitive. Thus, any kind of surface modifications devoted to functionalize them has to be finely controlled in terms of mass and optical characteristics, such as refractive index, to minimize the perturbation, on the transduced signal, that can affect the response sensitivity towards the detected target species. In this work, the study and optimization of ultra-thin plasma polymers and copolymers, compatible with these constrains and obtained from the vapours of acrylic acid containing a carboxylic (−COOH) group and styrene (an aromatic molecule with a vinyl as substituent at the ring), are reported. The obtained plasma polyacrylic acid (PPAA), plasma polystyrene (PPST) and their copolymer (PPAA-ST), characterized through optical contact angle analysis (OCA), Fourier transform infrared (FTIR) spectroscopy in attenuated total reflection (ATR-FTIR), X-ray photoelectrons spectroscopy (XPS), and atomic force microscopy (AFM), are shown to match specific and critical requirements, such as low thickness (∼40 nm) and refractive index (∼1.5), high surface density of reactive groups (10 15-10 16 COOH/cm 2), bioantifouling properties where required, reproducibility, and chemical resistance and stability.

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Plasma‐Polymerized Films for Biochip Design

Cited by 26 publications

References 74 publications

Wettability and Other Surface Properties of Modified Polymers

Wettability and Other Surface Properties of Modified Polymers

Enhancement of Polymer Cytocompatibility by Nanostructuring of Polymer Surface

Ultra-Thin Plasma-Polymerized Functional Coatings for Biosensing: Polyacrylic Acid, Polystyrene and Their Co-Polymer

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