Plasma Protective Effect on Red Blood Cells Exposed to Mechanical Stress

Kameneva, Marina V.; Antaki, James F.; Yeleswarapu, Krishna K.; Watach, Mary J.; Griffith, Bartley P.; Borovetz, Harvey S.

doi:10.1097/00002480-199703000-00186

Cited by 34 publications

(36 citation statements)

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“…Various plasma proteins have likewise demonstrated protective effects on RBC mechanical damage (64). In these studies, bovine cells suspended in various solutions were simultaneously exposed to the same mechanical stress using the standard RBC mechanical fragility test (as described above) in experiments performed at room temperature with controlled osmolality and viscosity of the suspension media.…”

Section: Protection Of Red Blood Cells From Mechanical Traumamentioning

confidence: 99%

Mechanical Blood Trauma in Assisted Circulation: Sublethal RBC Damage Preceding Hemolysis

et al. 2016

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After many decades of improvements in mechanical circulatory assist devices (CADs), blood damage remains a serious problem during support contributing to variety of adverse events, and consequently affecting patient survival and quality of life. The mechanisms of cumulative cell damage in continuous-flow blood pumps are still not fully understood despite numerous in vitro, in vivo, and in silico studies of blood trauma. Previous investigations have almost exclusively focused on lethal blood damage, namely hemolysis, which is typically negligible during normal operation of current generation CADs. The measurement of plasma free hemoglobin (plfHb) concentration to characterize hemolysis is straightforward, however sublethal trauma is more difficult to detect and quantify since no simple direct test exists. Similarly, while multiple studies have focused on thrombosis within blood pumps and accessories, sublethal blood trauma and its sequelae have yet to be adequately documented or characterized. This review summarizes the current understanding of sublethal trauma to red blood cells (RBCs) produced by exposure of blood to flow parameters and conditions similar to those within CADs. It also suggests potential strategies to reduce and/or prevent RBC sublethal damage in a clinically-relevant context, and encourages new research into this relatively uncharted territory.

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Section: Protection Of Red Blood Cells From Mechanical Traumamentioning

confidence: 99%

Mechanical Blood Trauma in Assisted Circulation: Sublethal RBC Damage Preceding Hemolysis

et al. 2016

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“…Several studies have indicated that RBCs from male humans and mice exhibit increased susceptibility to stress-induced hemolysis after cold storage. 3,6,19,20 Recent studies implicated testosterone effects on RBC production as a mechanism for this sex effect. 6 In addition, a recent observational study has associated female sex and a young donor age with increased risk of posttransfusion mortality.…”

Section: Introductionmentioning

confidence: 99%

Ethnicity, sex, and age are determinants of red blood cell storage and stress hemolysis: results of the REDS-III RBC-Omics study

et al. 2017

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Genetic polymorphisms in blood donors may contribute to donor-specific differences in the survival of red blood cells (RBCs) during cold storage and after transfusion. Genetic variability is anticipated to be high in donors with racial admixture from malaria endemic regions such as Africa and Asia. The purpose of this study was to test the hypothesis that donor genetic background, reflected by sex and self-reported ethnicity, significantly modulates RBC phenotypes in storage. High throughput hemolysis assays were developed and used to evaluate stored RBC samples from 11 115 African American, Asian, white, and Hispanic blood donors from 4 geographically diverse regions in the United States. Leukocyte-reduced RBC concentrate-derived samples were stored for 39 to 42 days (1–6°C) and then evaluated for storage, osmotic, and oxidative hemolysis. Male sex was strongly associated with increased susceptibility to all 3 hemolysis measures (P < .0001). African American background was associated with resistance to osmotic hemolysis compared with other racial groups (adjusted P < .0001). Donor race/ethnicity was also associated with extreme (>1%) levels of storage hemolysis exceeding US Food and Drug Administration regulations for transfusion (hemolysis >1% was observed in 3.51% of Asian and 2.47% of African American donors vs 1.67% of white donors). These findings highlight the impact of donor genetic traits on measures of RBC hemolysis during routine cold storage, and they support current plans for genome-wide association studies, which may help identify hereditable variants with substantive effects on RBC storage stability and possibly posttransfusion outcomes.

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“…Note that the MFI of the washed RBCs might have been somewhat exaggerated by the reduced amount of plasma proteins in the liquid milieu surrounding these cells as they were being rocked. As plasma proteins seem to help maintain the integrity of RBC membranes under sheer stress, 17 their absence might have made the washed RBCs slightly more susceptible to hemolysis during MFI measurement compared to the unwashed RBCs, which were rocked in a protein‐rich plasma environment.…”

Section: Discussionmentioning

confidence: 99%

Hemolysis and red blood cell mechanical fragility in shed blood after total knee arthroplasty

2011

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Plasma Protective Effect on Red Blood Cells Exposed to Mechanical Stress

Cited by 34 publications

References 0 publications

Mechanical Blood Trauma in Assisted Circulation: Sublethal RBC Damage Preceding Hemolysis

Mechanical Blood Trauma in Assisted Circulation: Sublethal RBC Damage Preceding Hemolysis

Ethnicity, sex, and age are determinants of red blood cell storage and stress hemolysis: results of the REDS-III RBC-Omics study

Hemolysis and red blood cell mechanical fragility in shed blood after total knee arthroplasty

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