Plasma proteins and leukocyte kinetics of turtles (Podocnemis unifilis (Troschel, 1848)) inoculated with inactivated Escherichia coli

Costa, Maria Beatriz Fraga; Costa, Regina Mamede; Boldrin, Marcillo Altoé; Neto, Evandro Pereira; Júnior, Paulo Dias Ferreira; Santos, Marcelo Renan de Deus; Lenz, Dominik

doi:10.1007/s00580-019-03059-x

Cited by 1 publication

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References 9 publications

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“…This is the first study about the effects of heparin sodium and EDTA (sodium and potassium) as anticoagulants on the hematological parameters of P. unifilis. The use of sodium heparin in the hematological evaluation of turtles and other reptiles has been widely described in several studies (AGUIRRE et al, 1995;MAFUVADZE & ERLWANGER, 2007;SANTOS et al, 2009;SABINO et al, 2010, ISHIKAWA et al, 2010, COSTA et al, 2020, and has been found to be an effective anticoagulant. Blood clotting inhibition was efficient in all the tests with the anticoagulants evaluated.…”

Section: Different Anticoagulants Effects On P Unifilis Blood Samplementioning

confidence: 99%

Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

et al. 2022

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Knowledge of suitable methods and reagents for assessing the health condition of specimens of a given species is essential. The present study evaluated the efficacy of the heparin anticoagulants 5,000 I.U. mL-1, Na2EDTA, and K3EDTA on the blood parameters of yellow-spotted amazon river turtle Podocnemis unifilis, employing different solutions for red blood cells count. The use of the various anticoagulants evaluated after 10 hours of storage efficiently inhibited coagulation in blood samples from P. unifilis. An increased number of erythrocytes was observed with the use of K3EDTA 5% when compared with heparin. Statistically significant changes in the erythrocyte number were observed with the use of the different solutions. Solutions which featured sodium citrate and formaldehyde in their composition, allowed erythrocytes counting up to 120 hours after blood collection, without a change in values. The use of the heparin anticoagulants 5,000 I.U. mL-1, Na2EDTA 3%, Na2EDTA 5%, K3EDTA 3% was recommended in the hematological analysis of P. unifilis. Also recommended was the use of the formaldehyde-citrate solution containing 1.9 g of sodium citrate and 1.0 mL of formaldehyde (in 50 mL of distilled water) to perform red blood cells counts in yellow-spotted amazon river turtle.

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Section: Different Anticoagulants Effects On P Unifilis Blood Samplementioning

confidence: 99%

Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

et al. 2022

View full text Add to dashboard Cite

show abstract

Plasma proteins and leukocyte kinetics of turtles (Podocnemis unifilis (Troschel, 1848)) inoculated with inactivated Escherichia coli

Cited by 1 publication

References 9 publications

Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

Anticoagulants and their effects on the hematological and biochemical parameters of yellow-spotted amazon river turtle

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