Plasma proteins of rainbow trout (Oncorhynchus mykiss) isolated by binding to lipopolysaccharide from Aeromonas salmonicida

Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection

“…Jorgensen et al [25] also reported a similar up-regulation in serum amyloid A (SAA) gene expression in the hepatocytes of S. salar stimulated with LPS. Although CRP is known to bind to LPS [26,46,47], our results may suggest that a CRP acute response was not triggered in the control fed fish by LPS injection. This is in accordance with previous studies conducted in common carp injected with LPS (3 mg/kg) where an absence of a typical large CRP acute phase reaction was noted by MacCarthy et al [26].…”

Isolation and characterisation of a serum lectin from blue gourami, Trichogaster trichopterus(Pallus)

“…These two components also showed strong specificities towards N-acetyl-Dgalactosamine and N-acetyl-D-glucosamine. The native molecular masses of the purified BGL, being two isoforms of 65 and 66 kDa, are relatively small as compared to other fish serum lectins which have molecular masses over 100 kDa (Manihar et al, 1991;Hoover et al, 1998). In addition, the purified BGL isoforms were found to share the same N-terminal sequence (HGEENRXGPR), an isoelectric point of 5·6 and show strong carbohydrate specificity towards N-acetyl-D-galactosamine.…”

Nonspecific interactions alter lipopolysaccharide patterns and protein mobility on sodium dodecyl sulfate polyacrylamide gels

“…mophilus influenzae type b [19], and several plasma proteins of rainbow trout [20]. More pertinent to the present study, however, were LPS-protein interactions resistant to dissociation under harsh conditions (i.e., SDS, urea, and high temperature).…”