Plasma Rich in Growth Factors Enhances Cell Survival after in Situ Retinal Degeneration

Suárez-Barrio, Carlota; Olmo-Aguado, Susana del; García-Pérez, Eva; Artime, Enol; Fuente, María de la; Muruzábal, Francisco; Anitua, Eduardo; Baamonde-Arbaiza, Begoña; Fernández-Vega, Luís; Merayo‐Lloves, Jesús

doi:10.3390/ijms21207442

Cited by 4 publications

(2 citation statements)

References 64 publications

(86 reference statements)

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“…In this regard, Anitua et al have recently developed a fibrin membrane with improved optical properties that can act as a drug-delivering system [ 30 ]. The different formulations obtained from PRGF technology have been shown to have regenerative, anti-apoptotic, anti-inflammatory, and anti-oxidative stress effects [ 15 , 27 , 28 ]. In comparison to PRGF, the proteomic analysis of HK cells exposed to autologous serum has shown increased expression of inflammation, fibrosis, angiogenesis, and oxidative stress [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…In recent studies, both membrane and injectable formulations obtained from PRGF technology have been used for the treatment of macular holes, paving the way for the treatment of other vitreoretinal diseases [ 23 , 24 , 25 ]. An interesting effect of PRGF in this context is its ability to reduce oxidative stress and to improve the survival of retinal pigment epithelial cells exposed to blue light [ 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells

Anitua

Muruzábal

Fuente

et al. 2023

IJMS

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The present study evaluates the ability of a novel plasma rich in growth factors (PRGF) membrane with improved optical properties to reduce oxidative stress in retinal pigment epithelial cells (ARPE-19 cells) exposed to blue light. PRGF was obtained from three healthy donors and divided into four main groups: (i) PRGF membrane (M-PRGF), (ii) PRGF supernatant (S-PRGF), (iii) platelet-poor plasma (PPP) membrane diluted 50% with S-PRGF (M-PPP 50%), and (iv) M-PPP 50% supernatant (S-PPP 50%). ARPE-19 cells were exposed to blue light and then incubated with the different PRGF-derived formulations or control for 24 and 48 h under blue light exposure. Mitochondrial and cell viability, reactive oxygen species (ROS) production, and heme oxygenase-1 (HO-1) and ZO-1 expression were evaluated. Mitochondrial viability and cell survival were significantly increased after treatment with the different PRGF-derived formulations. ROS synthesis and HO-1 expression were significantly reduced after cell treatment with any of the PRGF-derived formulations. Furthermore, the different PRGF-derived formulations significantly increased ZO-1 expression in ARPE-19 exposed to blue light. The new PRGF membrane with improved optical properties and its supernatant (M-PPP 50% and S-PPP 50%) protected and reversed blue light-induced oxidative stress in ARPE-19 cells at levels like those of a natural PRGF membrane and its supernatant.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells

Anitua

Muruzábal

Fuente

et al. 2023

IJMS

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IL-17A enhances the inflammatory response of glaucoma through Act1/TRAF6/NF-κB pathway

Zheng,

Mou,

Tan

et al. 2024

Neurochemistry International

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Plasma Rich in Growth Factors Promotes Autophagy in ARPE19 Cells in Response to Oxidative Stress Induced by Blue Light

et al. 2021

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Age-related macular degeneration (AMD) causes the degeneration of photoreceptors and retinal cells leading to vision loss in older subjects. Among possible exogenous risk factors, it has been recently proposed that long-term exposure to blue light could aggravate the course of AMD. In the search for therapeutic options, plasma rich in growth factors (PRGF) has been shown to enhance cell antioxidant pathways and protect photoreceptors against the harm produced by blue light, although its mechanism of action remains unknown. One possible mechanism, autophagy, is one of the most conservative cell renewal systems used in eukaryotes to destroy cellular components that have been damaged by some kind of insult. The oxidative stress of exposure to blue light is known to induce cell autophagy. In this study, we examined the combined effects on autophagy of blue light and PRGF in a retinal cell line, ARPE19. In response to treatment with both PRGF and blue light, we detected the modulated expression of autophagy markers such as NF-kB, p62/sqstm1, Atg5, LC3 and Beclin1, and inflammatory markers such as IL1B and IL18. Our findings suggest that PRGF promotes cell autophagy in response to exposure to blue light.

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Plasma Rich in Growth Factors Enhances Cell Survival after in Situ Retinal Degeneration

Cited by 4 publications

References 64 publications

PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells

PRGF Membrane with Tailored Optical Properties Preserves the Cytoprotective Effect of Plasma Rich in Growth Factors: In Vitro Model of Retinal Pigment Epithelial Cells

IL-17A enhances the inflammatory response of glaucoma through Act1/TRAF6/NF-κB pathway

Plasma Rich in Growth Factors Promotes Autophagy in ARPE19 Cells in Response to Oxidative Stress Induced by Blue Light

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