Plasma <scp>CXCL</scp>1 levels and <scp>TRAF</scp>3<scp>IP</scp>2 variants in patients with myocardial infarction

Pordel, Safoora; Khanian, Mahdi Sajedi; Karimi, Mohammad Hossein; Nikoo, Hossein; Doroudchi, Mehrnoosh

doi:10.1002/jcla.22402

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…Further, CXCL-1 is associated with coronary artery calcium (CAC), aortic wall thickness and plaque burden. 49 Our results are in contrast to the circulatory CXCL-1 levels from other studies. This might be a compensatory response to the type 2 diabetes with coronary atherosclerosis.…”

Section: Discussioncontrasting

confidence: 99%

Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients

Johny¹,

Bhaskar²,

Alam³

et al. 2021

JIR

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Background: Type 2 diabetes mellitus (T2DM) is a well-established risk factor for the development of atherosclerotic coronary artery disease. Platelet hyperactivity and inflammation are associated with the development of coronary artery disease (CAD) in T2DM patients. We investigated the status of immune cells, platelet activation, and plateletimmune cell interactions in T2DM_CAD patients. Methodology:The study population consisted of four groups of subjects, healthy control (CT, n = 20), T2DM (n = 44), CAD (n = 20) and T2DM_CAD (n = 38). Platelet activation, immunome profiling and platelet-immune cell interactions were analysed by flow cytometry. The circulatory levels of inflammatory cytokines/chemokines were assessed using multiplex assay. Results: Increased platelet activation and increased platelet-immune cell aggregate formation were observed in T2DM and T2DM_CAD groups compared to the control and CAD groups (p < 0.05). Our immunome profile analysis revealed, altered monocyte subpopulations and dendritic cell populations in T2DM, CAD and T2DM_CAD groups compared to the control group (p < 0.05). Furthermore, significantly increased IL-1β, IL-2, IL-4, IL-6, IL-8, IL12p70, IL-13 IL-18, CCL2, and decreased CXCL1, CCL5 levels were observed in T2DM_CAD group compared to the control group. Our ex-vivo study increased plateletmonocyte aggregate formation was observed upon D-glucose exposure in a time and concentration dependent manner. Conclusion: Our data suggests that T2DM, CAD and T2DM_CAD are associated with altered immune cell populations. Furthermore, it has been confirmed that hyperglycemia induces platelet activation and forms platelet-immune cell aggregation which may lead to the release of inflammatory cytokines and chemokines and contribute to the complexity of CAD and type 2 diabetes.

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Section: Discussioncontrasting

confidence: 99%

Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients

Johny¹,

Bhaskar²,

Alam³

et al. 2021

JIR

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“… 38–40 Plasma levels of CXCL1 are significantly increased in patients with AMI. 41 In the present study, we found negatively regulatory networks of miRNA–oxidative stress and prognosis-related mRNAs (hsa-miR-604-TLR4 and hsa-miR-139-5p-CXCL1) in AMI. This indicated that these miRNAs and targets play a crucial role in angiogenesis of AMI.…”

Section: Discussionsupporting

confidence: 54%

Screening for Regulatory Network of miRNA–Inflammation, Oxidative Stress and Prognosis-Related mRNA in Acute Myocardial Infarction: An in silico and Validation Study

Yin¹,

Wang²,

Wang³

et al. 2022

IJGM

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Background Acute myocardial infarction (AMI), which commonly leads to heart failure, is among the leading causes of mortality worldwide. The aim of this study was to find potential regulatory network for miRNA-inflammation, oxidative stress and prognosis-related mRNA to uncover molecular mechanisms of AMI. Methods The expression profiles of miRNA and mRNA in the blood samples from AMI patients were downloaded from the Gene Expression Omnibus (GEO) dataset for differential expression analysis. Weighted gene co-expression network analysis (WGCNA) was used to further identify important mRNAs. The negatively regulatory network construction of miRNA–inflammation, oxidative stress and prognosis-related mRNAs was performed, followed by protein–protein interaction (PPI) and functional analysis of mRNAs. Results A total of three pairs of negatively regulatory network of miRNA–inflammation and prognosis-related mRNAs (hsa-miR-636/hsa-miR-491-3p/hsa-miR-188-5p/hsa-miR-188-3p-AQP9, hsa-miR-518a-3p-C5AR1 and hsa-miR-509-3-5p/hsa-miR-127-5p-PLAUR), two pairs of negatively regulatory network of miRNA–oxidative stress and prognosis-related mRNAs (hsa-miR-604-TLR4 and hsa-miR-139-5p-CXCL1) and three pairs of negatively regulatory network of miRNA-inflammation, oxidative stress and prognosis-related mRNA (hsa-miR-634/hsa-miR-591-TLR2, hsa-miR-938-NFKBIA and hsa-miR-520h/hsa-miR-450b-3p-ADM) were identified. In the KEGG analysis, some signaling pathways were identified, such as complement and coagulation cascades, pathogenic Escherichia coli infection, chemokine signaling pathway and cytokine–cytokine receptor interaction and Toll-like receptor signaling pathway. Conclusion Identified negatively regulatory network of miRNA-inflammation/oxidative stress and prognosis-related mRNA may be involved in the process of AMI. Those inflammation/oxidative stress and prognosis-related mRNAs may be diagnostic and prognostic biomarkers for AMI.

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“…And the plasma levels of CXCL1 were significantly correlated with TT genotype of TRAF3IP2 (rs33980500) in myocardial infarction patients. [ 47 ] Whereas, CXCL8 may mediate the recruitment and activation of neutrophils, and promote the formation of new blood vessels. [ 48 ] In addition, CXCL8 is upregulated in the infarct area inducing polymorphonuclear leukocyte infiltration.…”

Section: Discussionmentioning

confidence: 99%

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

Guo

Zhou

et al. 2021

Medicine

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Background: Acute myocardial infarction (AMI) is a common disease leading threat to human health around the world. Here we aimed to explore new biomarkers and potential therapeutic targets in AMI through adopting integrated bioinformatics tools. Methods: The gene expression Omnibus (GEO) database was used to obtain genes data of AMI and no-AMI whole blood. Furthermore, differentially expressed genes (DEGs) were screened using the “Limma” package in R 3.6.1 software. Functional and pathway enrichment analyses of DEGs were performed via “Bioconductor” and “GOplot” package in R 3.6.1 software. In order to screen hub DEGs, the STRING version 11.0 database, Cytoscape and molecular complex detection (MCODE) were applied. Correlation among the hub DEGs was evaluated using Pearson's correlation analysis. Results: By performing DEGs analysis, 289 upregulated and 62 downregulated DEGs were successfully identified from GSE66360, respectively. And they were mainly enriched in the terms of neutrophil activation, immune response, cytokine, nuclear factor kappa-B (NF-κB) signaling pathway, IL-17 signaling pathway, and tumor necrosis factor (TNF) signaling pathway. Based on the data of protein–protein interaction (PPI), the top 10 hub genes were ranked, including interleukin-8 (CXCL8), TNF, N-formyl peptide receptor 2 (FPR2), growth-regulated alpha protein (CXCL1), transcription factor AP-1 (JUN), interleukin-1 beta (IL1B), platelet basic protein (PPBP), matrix metalloproteinase-9 (MMP9), toll-like receptor 2 (TLR2), and high affinity immunoglobulin epsilon receptor subunit gamma (FCER1G). What's more, the results of correlation analysis demonstrated that there was positive correlation between the 10 hub DEGs. Conclusion: Ten DEGs were identified as potential candidate diagnostic biomarkers for patients with AMI in present study. However, further experiments are needed to confirm the functional pathways and hub genes associated with AMI.

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Plasma CXCL1 levels and TRAF3IP2 variants in patients with myocardial infarction

Abstract: The gene variants of Act1 adaptor are associated with correlates of poor outcome in patients with MI and plasma CXCL1 levels.

Cited by 10 publications

References 35 publications

Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients

Platelet Mediated Inflammation in Coronary Artery Disease with Type 2 Diabetes Patients

Screening for Regulatory Network of miRNA–Inflammation, Oxidative Stress and Prognosis-Related mRNA in Acute Myocardial Infarction: An in silico and Validation Study

Identification and analysis of key genes associated with acute myocardial infarction by integrated bioinformatics methods

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