Plasma storage at −80°C does not protect matrix metalloproteinase-9 from degradation

Rouy, Didier; Ernens, Isabelle; Jeanty, Céline; Wagner, Daniel R.

doi:10.1016/j.ab.2004.10.052

Cited by 88 publications

(64 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among other MMPs, MMP-9 is known to be self-activating, with the cleavage of the propeptide leading to the formation of an active enzyme (43). TIMP-1, the natural inhibitor of MMP-9 and MMP-2 (43), is secreted by dNK (25,36), a finding that we have confirmed.…”

Section: Discussionsupporting

confidence: 78%

See 1 more Smart Citation

Decidual NK Cells Alter In Vitro First Trimester Extravillous Cytotrophoblast Migration: A Role for IFN-γ

Dutz

MacCalman

et al. 2006

The Journal of Immunology

124

110

View full text Add to dashboard Cite

Abnormal placentation results in either inadequate (consequences: recurrent miscarriage, intrauterine growth restriction, and preeclampsia) or overzealous (consequences: placenta accreta, increta, and percreta) placentation. NK cells dominate in first trimester decidua and probably control extravillous cytotrophoblast (EVT) invasion. We examined this interaction in a novel way, using NK cells and villous explants from concordant first trimester pregnancies cocultured using a new collagen (two-dimensional) model of placentation. Decidual NK (dNK) cells exerted contact-independent inhibition of normal cytotrophoblast migration, associated with changes in the cytotrophoblast expression of metalloproteases-2 and -9, and plasminogen activator inhibitor-1. dNK cells did not affect EVT proliferation and apoptosis, and cell column formation. dNK cell effects were partially reversed by neutralizing Abs against IFN-γ. We provide ex vivo human evidence of a direct role for dNK in modulating EVT differentiation as they form columns and then migrate from anchoring villi.

show abstract

Section: Discussionsupporting

confidence: 78%

“…TIMP-1, the natural inhibitor of MMP-9 and MMP-2 (43), is secreted by dNK (25,36), a finding that we have confirmed. TIMP-1 can block the activation of pro-MMP-9 in skin (37).…”

Section: Discussionsupporting

confidence: 78%

Decidual NK Cells Alter In Vitro First Trimester Extravillous Cytotrophoblast Migration: A Role for IFN-γ

Dutz

MacCalman

et al. 2006

The Journal of Immunology

124

110

View full text Add to dashboard Cite

show abstract

“…Our tubes were stored at -80°C before MMP-9 assay. However, the stability of MMP-9 at -80°C is a matter of debate [58,59]. Splenocytes from wild-type or MMP-9-deficient mice were stimulated with ConA (10 mg/ml).…”

Section: Discussionmentioning

confidence: 99%

Soluble interleukin-2 receptor and metalloproteinase-9 expression in head and neck cancer: prognostic value and analysis of their relationships

Agueznay

Badoual

Hans

et al. 2007

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryIn a series of 84 head and neck patients, a statistically significant correlation was observed between high serum soluble interleukin (IL)-2 receptor alpha (sIL-2Ra) (P = 0·034) and metalloproteinase-9 (MMP-9) concentrations (P = 0·036) at diagnosis and a shorter survival of these patients. As MMP-9 has been shown to mediate cleavage of IL-2Ra (CD25) by preactivated T cells, we looked for a relationship between MMP-9 expression and soluble IL-2Ra serum concentrations in these cancer patients. We did not find any correlation between intratumoral expression of MMP-9 or serum MMP-9 concentrations and serum sIL-2Ra levels. These results led us to reassess the role of MMP-9 in the release of sIL-2Ra. Treatment of Kit225 leukaemic cells with recombinant MMP-9 slightly decreased membrane CD25 expression and was associated with an increased concentration of sIL-2Ra in the supernatants. However, using a selective inhibitor of MMP-9 we did not succeed in specifically inhibiting the release of sIL-2Ra by the Kit225 cell line or by phytohaemagglutinin (PHA)-activated peripheral blood mononuclear cells. In addition, in a preclinical mouse model, basal serum sIL-2Ra concentrations and sIL-2Ra production by activated cells were not altered in MMP-9-deficient mice compared to wild-type mice. Interestingly, a broad spectrum metalloproteinase inhibitor inhibited the release of sIL-2Ra by PHAactivated peripheral blood mononuclear cells, suggesting that in contrast with current views concerning the major role of MMP-9 in the cleavage of membrane IL-2Ra, other proteases are involved in the shedding of sIL-2Ra. MMP-9 and sIL-2Ra appear therefore as independent prognostic markers in head and neck cancers.

show abstract

“…In our experience and as reported previously, the results of assessment of some MMPs may be influenced significantly by the duration of storage. 30 …”

Section: Study Limitationsmentioning

confidence: 99%

Extracellular Cardiac Matrix Biomarkers in Patients With Acute Myocardial Infarction Complicated by Left Ventricular Dysfunction and Heart Failure

et al. 2009

View full text Add to dashboard Cite

Background-Aldosterone stimulates cardiac collagen synthesis. Circulating biomarkers of collagen turnover provide a useful tool for the assessment of cardiac remodeling in patients with congestive heart failure and left ventricular systolic dysfunction after acute myocardial infarction. Methods and Results-In a substudy of the Eplerenone Post-Acute Myocardial Infarction Heart Failure Efficacy and Survival Study (EPHESUS), which evaluated the effects of the selective aldosterone receptor antagonist eplerenone versus placebo, serum levels of collagen biomarkers were measured in 476 patients with congestive heart failure after acute myocardial infarction complicated with left ventricular systolic dysfunction. The combination of the type I collagen telopeptide and brain natriuretic peptide levels above median at baseline was associated with all-cause mortality and the composite end point of cardiovascular death or heart failure hospitalization, with hazard ratios of 2.49 (Pϭ0.039) and 3.03 (Pϭ0.002), respectively. During follow-up, levels of aminoterminal propeptide of type I and type III procollagen were found to be consistently lower in the eplerenone group and significantly lower beginning at 6 months. Conclusions-Changes in biomarkers of collagen synthesis and degradation suggest that extracellular matrix remodeling is an active process in patients with congestive heart failure and left ventricular systolic dysfunction after acute myocardial infarction. High type I collagen telopeptide and high brain natriuretic peptide serum levels are associated with the highest event rate. Eplerenone suppresses post-acute myocardial infarction collagen turnover changes.

show abstract

Plasma storage at −80°C does not protect matrix metalloproteinase-9 from degradation

Cited by 88 publications

References 26 publications

Decidual NK Cells Alter In Vitro First Trimester Extravillous Cytotrophoblast Migration: A Role for IFN-γ

Decidual NK Cells Alter In Vitro First Trimester Extravillous Cytotrophoblast Migration: A Role for IFN-γ

Soluble interleukin-2 receptor and metalloproteinase-9 expression in head and neck cancer: prognostic value and analysis of their relationships

Extracellular Cardiac Matrix Biomarkers in Patients With Acute Myocardial Infarction Complicated by Left Ventricular Dysfunction and Heart Failure

Contact Info

Product

Resources

About