Plasma trapping in the centrifuged red cells of children with severe thalassaemia

Economou-Mavrou, C.; Tsenghi, Christina

doi:10.1136/jcp.18.2.203

Cited by 6 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M. England and M. C. Down have been observed in sickle cell anaemia (England et al, 1972). High plasma trapping has also been observed in severe thalassaeinia (Economou-Mavrou & Tsenghi, 1965)~ in hereditary spherocytosis (Furth, 1956), with the blood of some animals (Chien et al, 1965) and with fixed erythrocytes (Archer et a2, 1970).…”

mentioning

confidence: 98%

Determination of the Packed Cell Volume using ¹³¹I‐Human Serum Albumin*

England¹,

Down²

1975

Br J Haematol

View full text Add to dashboard Cite

When trace quantities of labelled albumin have been added to blood, the packed cell volume (PCV) may be determined by isotope counting using the relationship: (plasma counts-blood counts)/(plasma counts). This method for measuring the PCV is not affected by plasma trapping between the red cells and the results do not depend upon the centrifugation conditions. The PCV determination with labelled albumin has a high precision (coefficient of variation = 0.9%) and there is very good agreement with the centrifugation PCV using Wintrobe tubes after this measurement has been corrected for plasma trapped between the red cells. No significant compression of the red cells occurred when the blood samples were centrifugated in Wintrobe PCV tubes.

show abstract

mentioning

confidence: 98%

Determination of the Packed Cell Volume using ¹³¹I‐Human Serum Albumin*

England¹,

Down²

1975

Br J Haematol

View full text Add to dashboard Cite

show abstract

Internal Viscosity of the Red Blood Cell and the Capillary Inversion Phenomena as Factors in Oxygen Transport

Dintenfass

1970

Blood Oxygenation

View full text Add to dashboard Cite

Fluid trapping of erythrocytes under hypoosmolar conditions

Stäubli

Roessler

Straub

1987

Blut

View full text Add to dashboard Cite

125I albumin was used to assess the amount of trapped fluid after microhematocrit centrifugation of erythrocytes suspended in buffers of different osmolality. Surprisingly the total amount of trapped fluid per volume unit of packed erythrocytes decreased with decreasing osmolality of the suspending buffer despite erythrocyte swelling. However, if the contribution of the individual erythrocyte to the trapped fluid was calculated, the trapped fluid per erythrocyte did not change between 311 mosm/kg and 256 mosm/kg. For osmolalities below 256 mosm/kg a significant increase of trapped fluid was obtained. It is concluded that the packing ability of erythrocytes is not impaired in suspending fluid of moderate to severe infraphysiological tonicity. The daily clinical experience that considerable degrees of plasma hypoosmolality are tolerated in vivo without hemolysis or impairment of oxygen transport by erythrocytes may be explained by the excellent ability of shape adaptation of erythrocytes to each other and to other surfaces such as vascular endothelia. The method of trapped fluid determination might be of potential value as a complementary method in the evaluation of erythrocyte rheology if the amount of trapped fluid is related to the individual erythrocyte.

show abstract

Plasma trapping in the centrifuged red cells of children with severe thalassaemia

Cited by 6 publications

References 43 publications

Determination of the Packed Cell Volume using ¹³¹I‐Human Serum Albumin*

Determination of the Packed Cell Volume using ¹³¹I‐Human Serum Albumin*

Internal Viscosity of the Red Blood Cell and the Capillary Inversion Phenomena as Factors in Oxygen Transport

Fluid trapping of erythrocytes under hypoosmolar conditions

Contact Info

Product

Resources

About

Plasma trapping in the centrifuged red cells of children with severe thalassaemia

Cited by 6 publications

References 43 publications

Determination of the Packed Cell Volume using 131I‐Human Serum Albumin*

Determination of the Packed Cell Volume using 131I‐Human Serum Albumin*

Internal Viscosity of the Red Blood Cell and the Capillary Inversion Phenomena as Factors in Oxygen Transport

Fluid trapping of erythrocytes under hypoosmolar conditions

Contact Info

Product

Resources

About

Determination of the Packed Cell Volume using ¹³¹I‐Human Serum Albumin*

Determination of the Packed Cell Volume using ¹³¹I‐Human Serum Albumin*