Plasma von Willebrand Factor and Intestinal Ischaemia-Reperfusion Injury in Rats

Abu‐Zidan, Fikri M.; Farrant, Glenn; Zwi, L. Jonathan; Simovic, Misho O.; Day, T. D.; Bonham, Martin J. D.; Windsor, John A.

doi:10.1016/s0049-3848(99)00013-4

Cited by 6 publications

(7 citation statements)

References 23 publications

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“…In agreement with our data, other authors demonstrated in rats that the plasma concentration of vWF rose after 30 min of intestinal ischemia followed by 15 min of reperfusion (2). Moreover, hypoxia, and also reoxygenation, induced release of vWF endothelial secretory granules in cultured cells (30).…”

Section: Discussionsupporting

confidence: 93%

“…After treatment, the diameter, RBC velocity, and flow changes in arterioles were evaluated, as well as plasma lipid peroxidation, vascular permeability, leukocyte adhesion, and changes in perfused capillary length (PCL) (4,7). Experiments were also carried out to measure the influence of I/R, with and without treatment, on plasma vWF concentration (2). To investigate the mechanisms contributing to the effects of the PEG compounds, the hamster cheek pouch was treated with the NO synthase inhibitor N Gmonomethyl-L-arginine (L-NMMA) in addition to PEG-NO before I/R.…”

mentioning

confidence: 99%

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Polyethylene glycol and a novel developed polyethylene glycol-nitric oxide normalize arteriolar response and oxidative stress in ischemia-reperfusion

Bertuglia

Veronese²,

Pasut³

2006

American Journal of Physiology-Heart and Circulatory Physiology

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Polyethylene glycol (PEG) has been shown to repair cell membranes and, thus, inhibit free radical production in in vitro and in vivo models. We hypothesized that PEG and newly developed organic nitrate forms of PEG (PEG-NO) could repair endothelial dysfunction in ischemia-reperfusion (I/R) injury in the hamster cheek pouch visualized by intravital fluorescent microscopy. After treatments, we evaluated diameter and RBC velocity and flow in arterioles, as well as lipid peroxides in the systemic blood, perfused capillary length, vascular permeability, leukocyte adhesion, and amount of von Willebrand factor (vWF) in the blood after I/R injury. A control group was treated with 5,000- or 10,000-Da PEG, and three groups were treated with PG1 (1 NO molecule covalently bound to PEG, 5,170 Da), PG8 (8 NO molecules covalently bound to PEG, 11,860 Da), and PG16 (16 NO molecules covalently bound to PEG, 14,060 Da). All animals received 0.5 mg/0.5 ml. Lipid peroxides increased at 5 and 15 min of reperfusion, whereas diameter, RBC velocity, and blood flow decreased in arterioles after I/R injury. Vascular permeability, leukocyte adhesion, and vWF increased significantly. PEG and PG1 attenuated lipid peroxides and vasoconstriction during reperfusion and decreased leukocyte adhesion and vascular permeability. PG8 maintained lipid peroxides at normal levels, increased arteriolar diameter, flow, and perfused capillary length, and decreased vWF level and leukocyte adhesion (P< 0.05). PG16 was less effective than PG1 and PG8. In conclusion, PEG-NO shows promise as a compound that protects microvascular perfusion by normalizing the balance between NO level and excessive production of free radicals in endothelial cells during I/R injury.

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Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Polyethylene glycol and a novel developed polyethylene glycol-nitric oxide normalize arteriolar response and oxidative stress in ischemia-reperfusion

Bertuglia

Veronese²,

Pasut³

2006

American Journal of Physiology-Heart and Circulatory Physiology

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“…The vWF factor contributes to primary hemostasis by facilitating platelet attachment to subendothelial substances in an injured vessel wall, stimulating the formation of a primary platelet plug. In the circulation, vWF protects factor VIII from proteolysis, and also serves to direct F VIII to sites in need of hemostasis (1,2,15,16). With its multi-adhesive properties vWF is able to bind a number of different proteins (1).…”

Section: Discussionmentioning

confidence: 99%

“…Many mechanisms and factors trigger the acute release of vWF from endothelial cells: α-thrombin, plasminogen activator, plasmin, adrenalin, bradykinin, interleukin-1 and calcium ionophore all increase its release (1,15,16). Usually the mechanism of increase in the vWF level involves an increase of acute phase proteins, or is a result of endothelial injury (1,6,15,16). In our study vWF release was unrelated to the endothelial cell trauma induced by cryopexy, but there was a tendency to lower values of this factor in SRF before cryopexy.…”

Section: Discussionmentioning

confidence: 99%

Von Willebrand Factor in Subretinal Fluid

Malukiewicz-Wiśniewska¹,

Kotschy

2001

European Journal of Ophthalmology

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“…Increased levels of vWF have been associated with CAD (4) and acute coronary syndromes (5,6). Chronic smoking may be associated with increased levels of vWF (28), possibly due to its release during oxidative damage of endothelial cells (29). Therefore, combined, high-dose antioxidant treatment may reduce vWF levels four weeks.…”

Section: Vwfmentioning

confidence: 99%

Effects of antioxidant vitamins C and E on endothelial function and thrombosis/fibrinolysis system in smokers

Antoniades¹,

Tousoulis²,

Tentolouris³

et al. 2003

Thromb Haemost

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SummarySmoking is associated with endothelial dysfunction and abnormalities in thrombosis/fibrinolysis system, possibly through increased oxidative stress. In this study we investigated the effect of combined antioxidant treatment with vitamins C and E on endothelial function and plasma levels of plasminogen activator inhibitor (PAI-1), von Willebrand factor (vWF), tissue plasminogen activator (tPA) and factor VII (fVII), in smokers. Forty-one healthy smokers were randomly divided into 4 groups receiving vitamin C 2g/day (group A), vitamin C 2g/day plus vitamin E 400IU/day (group B), vitamin C 2g/day plus vitamin E 800IU/day (group C) or no antioxidants (controls, group D), for 4 weeks. Forearm blood flow was measured using venous occlusion strain-gauge plethysmography. Forearm vasodilatory response to reactive hyperemia (RH%) or to sublingual nitroglycerin administration (NTG%) were considered as indexes of endothelium dependent or independent dilation respectively. After treatment, RH% was increased only in groups B (p <0.05) and C (p <0.001) but not in groups A and D. Plasma levels of PAI-1 and vWF were decreased only in group C (p <0.05 for both), while PAI-1/tPA ratio was significantly decreased in both groups B and C (p <0.05 for both). NTG% and plasma levels of tPA and fVII remained invariable in all groups. In conclusion, combined administration of vitamin C and vitamin E at high dosages, improved endothelial function and decreased plasma levels of PAI-1, vWF and PAI-1/tPA ratio in chronic smokers.

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Plasma von Willebrand Factor and Intestinal Ischaemia-Reperfusion Injury in Rats

Cited by 6 publications

References 23 publications

Polyethylene glycol and a novel developed polyethylene glycol-nitric oxide normalize arteriolar response and oxidative stress in ischemia-reperfusion

Polyethylene glycol and a novel developed polyethylene glycol-nitric oxide normalize arteriolar response and oxidative stress in ischemia-reperfusion

Von Willebrand Factor in Subretinal Fluid

Effects of antioxidant vitamins C and E on endothelial function and thrombosis/fibrinolysis system in smokers

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