Plasmablasts With a Mucosal Phenotype Contribute to Plasmacytosis in Systemic Lupus Erythematosus

Mei, Henrik E.; Hahne, Stefanie; Redlin, A. J.; Hoyer, Bimba F.; Wu, Kaiyin; Baganz, Lisa; Lisney, Anna; Alexander, Tobias; Rudolph, Birgit; Dörner, Thomas

doi:10.1002/art.40181

Cited by 31 publications

(28 citation statements)

References 52 publications

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“…In order to assure specificity of the staining, we show how the fluorescent signal in the same sample can be blocked with unconjugated protein used in excess. This methodology has been used successfully to analyze B cells specific for TT ; cholera toxin B (CTB) ; Keyhole Limpet Hemocyanin (KLH) ; and Pentraxin‐3 (PTX‐3) . The possibility to down‐titrate binding of antigen‐specific cells (competitive assay principle) has long been recognized to ensure specificity.…”

Section: Flow Cytometric Phenotyping Of Cells Across Species and Tissuesmentioning

confidence: 99%

“…Whole blood from a patient with systemic lupus erythematosus (SLE) was diluted with PBS at room temperature, and subjected to density gradient centrifugation over Ficoll (GE Healthcare, Uppsala, Sweden) to isolate PBMC. Active SLE patients exhibit significant B lymphopenia, with increased frequencies and absolute numbers of PB/PC in peripheral blood . PBMC were washed with PBS/0.2% BSA, and stained at 4°C for 15 min with a cocktail of the following mAbs: CD19 (clone SJ25C1, BD), CD27 (clone 2E4; Sanquine), CD20 (L27, BD), CD14 (M5E2, BD), CD3 (UCHT1, BD), CD38 (HIT2, BD), CD138 (B‐B4, Miltenyi Biotec).…”

Section: Flow Cytometric Phenotyping Of Cells Across Species and Tissuesmentioning

confidence: 99%

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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

et al. 2019

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These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.

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Section: Flow Cytometric Phenotyping Of Cells Across Species and Tissuesmentioning

confidence: 99%

Section: Flow Cytometric Phenotyping Of Cells Across Species and Tissuesmentioning

confidence: 99%

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

et al. 2019

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“…Oral lesions are present in approximately 21% of patients with systemic lupus erythematous and are associated with increased disease activity and a worse prognosis . The pathogenesis of systemic lupus erythematous involves dysregulation of cellular, humoral, and innate immunity, with findings supportive of an overly active mucosal immune system with increases in IgA plasmablasts . Genotyping of 4001 patients for 16 loci associated with systemic lupus erythematous found an association with STAT4 and protection of systemic lupus erythematous patients from oral ulceration .…”

Section: Autoimmune/systemic Diseases With Associated Ulceration Of Tmentioning

confidence: 99%

Recurrent oral ulceration: Etiology, classification, management, and diagnostic algorithm

Bilodeau

Lalla

2019

Periodontology 2000

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| INTRODUC TI ONOwing to the anatomic location, rich vasculature, and various functions of the oral cavity, the oral mucosa is exposed to a variety of insults. These include trauma from chewing or oral appliances, local irritation secondary to foods/drugs, exposure to systemically ingested drugs (through the blood supply), infectious agents, and effects of autoimmune and other systemic diseases. These insults can result in a variety of oral manifestations, ranging from hyperkeratosis to lichenoid lesions to frank ulceration. This review will discuss the etiology, pathogenesis, diagnosis, and management of the more common conditions that can lead to recurrent oral ulceration.

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“…Several studies validated that increased PB/PC induction is a feature of active SLE 8 15 24 including the demonstration that the PC gene expression profile correlated with disease activity. 25 Subsequent studies dissecting subgroups of PB/PC in peripheral blood of SLE 26 characterised at least two subsets. One phenotype, expressing CCR10 and ß7, produced IgA, whereas another subtype, expressing CD62L, produced preferentially IgG.…”

Section: Abnormalities Of B Lineage Differentiation In Slementioning

confidence: 99%

Therapeutic implications of the anergic/postactivated status of B cells in systemic lupus erythematosus

et al. 2020

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Systemic lupus erythematosus (SLE) is characterised by numerous abnormalities in B lineage cells, including increased CD27++ plasmablasts/plasma cells, atypical CD27-IgD- B cells with increased CD95, spleen tyrosine kinase (Syk)++, CXCR5- and CXCR5+ subsets and anergic CD11c+Tbet+ age-associated B cells. Most findings, together with preclinical lupus models, support the concept of B cell hyperactivity in SLE. However, it remains largely unknown whether these specific B cell subsets have pathogenic consequences and whether they provide relevant therapeutic targets. Recent findings indicate a global distortion of B cell functional capability, in which the entire repertoire of naïve and memory B cells in SLE exhibits an anergic or postactivated (APA) functional phenotype. The APA status of SLE B cells has some similarities to the functional derangement of lupus T cells. APA B cells are characterised by reduced global cytokine production, diminished B cell receptor (BCR) signalling with decreased Syk and Bruton’s tyrosine kinase phosphorylation related to repeated in vivo BCR stimulation as well as hyporesponsiveness to toll-like receptor 9 engagement, but intact CD40 signalling. This APA status was related to constitutive co-localisation of CD22 linked to phosphatase SHP-1 and increased overall protein phosphatase activities. Notably, CD40 co-stimulation could revert this APA status and restore BCR signalling, downregulate protein tyrosine phosphatase transcription and promote B cell proliferation and differentiation. The APA status and their potential rescue by bystander help conveyed through CD40 stimulation not only provides insights into possible mechanisms of escape of autoreactive clones from negative selection but also into novel ways to target B cells therapeutically.

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Plasmablasts With a Mucosal Phenotype Contribute to Plasmacytosis in Systemic Lupus Erythematosus

Cited by 31 publications

References 52 publications

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Recurrent oral ulceration: Etiology, classification, management, and diagnostic algorithm

Therapeutic implications of the anergic/postactivated status of B cells in systemic lupus erythematosus

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