Plasmalogen in ram semen, and its role in sperm metabolism

Hartree, E. F.; Mann, T.

doi:10.1042/bj0710423

Cited by 107 publications

(40 citation statements)

References 11 publications

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“…Phospholipids and lipoproteins were lost from erythrocytes under a variety of conditions including thermal shock (Lovelock 1954(Lovelock , 1955. There are also reports ofloss oflipid from frozen bull spermatozoa (Pickett and Komarek 1964), and a 10% loss of plasmalogen from cold-shocked ram spermatozoa (Hartree and Mann 1959).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Cold Shock and Freeze-Thawing on Release of Phospholipids by Ram, Bull, and Boar Spermatozoa

Darin-Bennett¹,

Poulos²,

White³

1973

Aust. Jnl. Of Bio. Sci.

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The effects of cold shock and freeze-thawing on the release of total phospholipid phosphorus and of specific phospholipids from ram, bull, and boar spermatozoa are examined. Species differences are apparent, both in the absolute amount of total phospholipids released and in the conditions required to effect a loss of individual phospholipids. The phosphoglycerides most affected by the temperature treatment are choline plasmalogen, phosphatidyl choline, and phosphat idyl ethanolamine. The loss of phospholipids is most specific in boar spermatozoa. Bull spermatozoa suffer a greater overall and more general breakdown of phospholipids, indicating disruption of the cell structure. This is in contrast to the more specific phospholipid losses from ram and boar spermatozoa, which may indicate a more localized region of damage and release of material, possibly acrosomal. The loss of phospholipid may be correlated with the species differences in the fertility of the frozen spermatozoa.

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Section: Introductionmentioning

confidence: 99%

The Effect of Cold Shock and Freeze-Thawing on Release of Phospholipids by Ram, Bull, and Boar Spermatozoa

Darin-Bennett¹,

Poulos²,

White³

1973

Aust. Jnl. Of Bio. Sci.

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“…Lecithin and choline plasmalogen were the major phospholipids observed in ram semen (Lovern, Olley, Hartree & Mann, 1957;Hartree & Mann, 1959, 1960 Gray, 1960;Quinn & White, 1967). Bovine semen contained up to seven classes of lipid of which 60 to 74% was phospholipid (Miller & Mayer, 1965;Komarek, Pickett, Lanz & Jensen, 1964;Pursel & Graham, 1967).…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Porcine Spermatozoa and Seminal Plasma Phospholipids as Affected by Frequency of Ejaculation

Johnson¹,

Gerrits²,

Young³

1969

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“…The retention of a pool of "cold" acetate after incubation for 3 hI' suggests that oxidation of plasmalogens (Hartree and Mann 1959, 1960a, 1960b, 1961 occurs in the presence of added fructose, although to a limited extent (O'Shea and Wales 1966). Such formation of metabolites from unlabelled endogenous substrates by spermatozoa would explain the reported accumulation of acids in some experiments (Vera Cruz, Lodge, and Graves 1967) but not in others where formation from labelled hexoses was measured (Wales and Humphries 1969).…”

Section: (C) I Dent~fication Of M Etabolite8mentioning

confidence: 85%

Effects of Potassium Plus Magnesium and of Aeroblc or Anaerobic Conditions on the Metabolites Produced From Fructose by Washed Ram and Bull Spermatozoa

O’Shea¹

1971

Aust. Jnl. Of Bio. Sci.

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The main metabolites from fructose were carbon dioxide, lactate, and acetate. Generally the effects of the addition of potassium (1 mM) plus magnesium (2 roM) were greater with 1 x 108 ram spermatozoa per millilitre than with 4 X 108 cells per millilitre.Lactate, which accumulated mostly in the incubation medium, was lost from the cells during their separation by gradient centrifugation. Addition of ions to dilute suspensions of ram spermatozoa increased the extracellular lactate.In contrast, acetate was not removed from the cell by the washing procedure and was mainly intracellular with aerobic conditions but was extracellular after anaerobic incubation. Addition of potassium and magnesium during aerobic incuba· tion increased the proportion of acetate found within the cell and this increase was greater with the more dilute suspension of ram spermatozoa. More intracellular acetate accumulated under aerobic than under anaerobic conditions and relatively more was found in the cells from the more dilute suspensions. However, the total amount of substrate carbon inside the cells was unaltered by changes in the condi· tions of incubation.Extracellular acetate was greater under anaerobic conditions. addition of potassium and magnesium had no effect with anaerobic these ions decreased extracellular acetate under aerobic conditions. effect was not as great with 4 X 108 spermatozoa per flask. While the incubation Again thisWashed ram spermatozoa also formed acetate (rom endogenous material, in the presence of added fructose.Washed bull spermatozoa accumulated acetate from fructose but more was extracellular than intracellular, and the amount and site of accumulation of acetate were not affected by addition of ions or by anaerobic conditions. Only 15-20% of the intracellular pool of substrate carbon in bull spermatozoa was acetate. As with ram spermatozoa, most lactate was found in the medium, with very little being intracellular.

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Plasmalogen in ram semen, and its role in sperm metabolism

Cited by 107 publications

References 11 publications

The Effect of Cold Shock and Freeze-Thawing on Release of Phospholipids by Ram, Bull, and Boar Spermatozoa

The Effect of Cold Shock and Freeze-Thawing on Release of Phospholipids by Ram, Bull, and Boar Spermatozoa

Quantitative Analysis of Porcine Spermatozoa and Seminal Plasma Phospholipids as Affected by Frequency of Ejaculation

Effects of Potassium Plus Magnesium and of Aeroblc or Anaerobic Conditions on the Metabolites Produced From Fructose by Washed Ram and Bull Spermatozoa

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