Plasmalogen phospholipids in plasma lipoproteins of normolipidemic donors and patients with hypercholesterolemia treated by LDL apheresis

Bräutigam, Carola; Engelmann, Bernd; Reiss, Daniela; Reinhardt, Ulrike; Thiery, Joachim; Richter, Werner; Brosche, Thorolf

doi:10.1016/0021-9150(95)05632-7

Cited by 61 publications

(43 citation statements)

References 22 publications

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“…We also characterized a number of minor phospholipid classes in the LDL. The PEs observed were predominantly etherlinked PE species, with less than one-third corresponding to acyl-PE, in agreement with previous fi ndings in LDL from normolipidemic individuals ( 51 ). In tissues, etherPEs have been suggested to act as antioxidants by chelating cupric ions and protecting other PLs from oxidation ( 52,53 ), but their function in LDL is as yet unknown.…”

Section: Evaluation Of Lipid Extractability Using Data Set 2 (Data Frsupporting

confidence: 90%

A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL

Reis

Rudnitskaya

Blackburn

et al. 2013

Journal of Lipid Research

214

200

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This article is available online at http://www.jlr.org functions, in addition to structural roles ( 1 ); consequently lipidomic studies have become fundamental for understanding their contribution to health and disease. Mass spectrometry (MS), with its capability of providing structural information, has been the main method of choice in lipidomic studies. Recent technological advances in mass spectrometers, including increased sensitivity, higher mass accuracy, higher scan speeds, and the ability to acquire in both positive and negative mode in one run have resulted in the increased popularity of MS as a detection technique for biomolecules in recent years. This has enabled the mapping of lipids present in fl uids and cells, leading to better understanding of the role of the different lipid classes in the pathophysiology of diseases.A critical step in lipidomic analysis is lipid extraction with an appropriate organic solvent mixture (solvent system) prior to MS detection. The solvent system should be capable of effectively extracting lipids representative of the sample under study without bias, inducing or promoting the degradation of lipids, or introducing contamination by nonlipid components such as sugars, peptides, and amino acids. Therefore, the success in the identifi cation and profi ling of lipids is critically dependent on the efficiency of the extraction step. The performance of the lipid extraction for a given sample (tissue, cell, or fl uid) with a particular solvent system depends on the partitioning of the

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Section: Evaluation Of Lipid Extractability Using Data Set 2 (Data Frsupporting

confidence: 90%

A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL

Reis

Rudnitskaya

Blackburn

et al. 2013

Journal of Lipid Research

214

200

View full text Add to dashboard Cite

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“…Plasmalogens in serum are biosynthesized in liver and secreted to circulating blood as lipoproteins components. The distribution of plasmalogens is approximately equal throughout all lipoprotein density fractions in human plasma 1,2) . Plasmalogens in serum are mainly classified into two types of glyc-erophospholipids such as ethanolamine plasmalogen (EtnPlas) and choline plasmalogen (ChoPlas), in which the former is a precursor of the latter in biosynthesis.…”

Section: Introductionmentioning

confidence: 97%

Plasmalogens in Human Serum Positively Correlate with High- Density Lipoprotein and Decrease with Aging

Maeba

Maeda

Kinoshita

et al. 2007

J Atheroscler Thromb

101

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Aim:The objective of the present study was to propose plasmalogens as a beneficial factor in human plasma by showing a highly positive correlation with high-density lipoprotein (HDL) and a significant reduction with aging. Methods: For 148 elderly subjects suspected of coronary artery disease (CAD), clinical characteristics such as coronary stenosis, hyperlipidemia, abnormal glucose tolerance, and hypertension were investigated, and serum biochemical markers including plasmalogens were determined. Results: Serum plasmalogens levels tended to fall in significant coronary stenosis and abnormal glucose tolerance. Correlative analyses among serum biochemical markers revealed that plasmalogens positively correlate with HDL-related values, particularly apolipoprotein A-(apo A-), and that the molar ratio of choline plasmalogen (ChoPlas) to ethanolamine plasmalogen (EtnPlas) correlates positively with low-density lipoprotein (LDL) particle size, and negatively with apo A-and fasting triglyceride (TG) levels. Comparison of plasmalogens in elderly subjects with those of 119 healthy young subjects showed a marked decrease in serum plasmalogens levels by aging. Conclusion: These results suggest that serum plasmalogens, antioxidant phospholipids, function as a beneficial factor as well as HDL, and that the measurement of serum plasmalogens is useful in clinical diagnosis.

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“…30 µmol\mmol of LDL cholesterol [7]. The percentage of plasmalogens in membranes of animal cells ranges in general between 15 and 20 % of total phospholipids, but can reach more than 50 % in the sarcolemma of some species [8].…”

Section: Introductionmentioning

confidence: 99%

“…The purity of the products, as determined by exposure to HCl fumes for 3-6 min, was between 93 and 95 %. A detailed description of the TLC procedure used to determine the plasmalogen content of phospholipids has been given elsewhere [7].…”

Section: Introductionmentioning

confidence: 99%

Delayed oxidative degradation of polyunsaturated diacyl phospholipids in the presence of plasmalogen phospholipids in vitro

Reiss

Beyer

Engelmann

1997

Biochemical Journal

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142

114

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The oxidative degradation of plasmalogen (alkenylacyl) phospholipids was analysed in the absence and the presence of polyunsaturated ester phospholipids by "H-NMR and by chemical determination. Brain lysoplasmenylethanolamine (lyso-P-PE), brain P-PE and erythrocyte P-PE, containing an increasing number of intrachain double bonds at sn # , were oxidized with 2,2h-azobis-(2-amidinopropane hydrochloride) (AAPH ; 2 or 10 mM) in Triton X-100 micelles (detergent\phospholipid 1 : 5, mol\mol). The formation of two peroxyl radicals was accompanied by the degradation of approx. one molecule of brain lyso-P-PE. On oxidation of brain P-PE or erythrocyte P-PE (320 nmol) with 2 mM AAPH, the (α-vinyl) methine "H signal of the enol ether decreased more rapidly than the methine proton peak of intrachain double bonds. The rate of enol ether degradation increased in the order : erythrocyte P-PE brain P-PE brain lyso-P-PE. The disappearance of the polyunsaturated ester phospholipids 1-palmitoyl-2-arachidonoyl phosphatidylcholine (16 : 0\20 : 4-PC) and 1-palmitoyl-2-linoleoyl phosphatidylcholine

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Plasmalogen phospholipids in plasma lipoproteins of normolipidemic donors and patients with hypercholesterolemia treated by LDL apheresis

Cited by 61 publications

References 22 publications

A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL

A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL

Plasmalogens in Human Serum Positively Correlate with High- Density Lipoprotein and Decrease with Aging

Delayed oxidative degradation of polyunsaturated diacyl phospholipids in the presence of plasmalogen phospholipids in vitro

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