Plasmatic antioxidant capacity due to ascorbate using TEMPO scavenging and electron spin resonance

Piehl, Lidia Leonor; Facorro, Graciela; Huarte, M.; Desimone, Martín Federico; Copello, Guillermo Javier; Díaz, Luis Eduardo; Celis, Emilio Rubín de

doi:10.1016/j.cccn.2005.03.035

Cited by 10 publications

(8 citation statements)

References 27 publications

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“…To convert the decay rate into an ascorbate equivalent concentration, we prepared a calibration curve with ascorbate-TEMPOL samples at known ascorbate concentration. The final ascorbate equivalent concentrations were normalized to the values of control rats, set as 100%, to give relative antioxidant levels [19].…”

Section: Antioxidant Capacity Of Plasma and Heart Homogenatesmentioning

confidence: 99%

Vitamin C prevents zidovudine-induced NAD(P)H oxidase activation and hypertension in the rat

Papparella

Ceolotto

Bertó

et al. 2007

Cardiovascular Research

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Section: Antioxidant Capacity Of Plasma and Heart Homogenatesmentioning

confidence: 99%

Vitamin C prevents zidovudine-induced NAD(P)H oxidase activation and hypertension in the rat

Papparella

Ceolotto

Bertó

et al. 2007

Cardiovascular Research

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“…27,28 Another advantage is the application of spectrophotometric detection, especially when compared to high cost, high maintenance detectors for ESR, 10 for instance.…”

Section: Introductionmentioning

confidence: 99%

“…3 For this reason, the assessment of total antioxidant capacity (TAC) of biological fluids will be a useful biomedical tool that can assist the evaluation of physiological, environmental and nutritional factors that influence the human redox status. 4 In this regard, several human biological fluids have been used as samples for the in vitro evaluation of TAC, including plasma, [5][6][7][8][9][10][11][12] serum, [13][14][15][16] urine, 8,12,17 saliva, 15 cerebrospinal fluid, 18 tears, 19 breast milk 20 and also semen. 21 Various batch methodologies based on electron spin resonance (ESR), 10 electrochemical, 6,11 chemiluminescence (CL), 8,15,16 fluorometric, 7 and UV-Vis spectrophotometric 5,13,14 detection have been proposed.…”

Section: Introductionmentioning

confidence: 99%

High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

et al. 2011

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High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a microformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h -1 (microplate method) or of 15 h -1 (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method.

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“…The ascorbic acid serum status was determined by electron spin resonance (ESR) spectroscopy (X band ESR Spectrometer Bruker ECS 106, German) as described elsewhere [35]. TEMPO (2,2,5,5-tetramethyl-4-piperidin-1-oxyl) (Sigma Chemical Co., USA) stock solution (6.3 mmol/L) was prepared with 96% ethanol (Laboratorios Cicarelli, Argentina) and stored at 4ºC until needed.…”

Section: Tempo Scavenging Assaymentioning

confidence: 99%

Glycine-Stabilized Zinc Gluconate Has Similar Bioavailability than Zinc Sulfate In a Zinc Fortified Probiotic Food

Tesan¹

2011

TONUTRAJ

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Objective: In this study, we evaluated zinc gluconate stabilized with glycine (GZ) and sulfate (SZ) in fermented milk as vehicle. Zinc bioavailability was evaluated in an animal model (Sprague Dawley rats) for both zinc sources in the vehicle with a probiotic (Lactobacillus casei DN114001).Results: For growth parameters, higher weight gain and femur weight values were observed when probiotic and zinc were provided together, independent of the source (weight gain: SZ 81.4g±4.0g; GZ 81.8g±4.0g and 70.2g±12.5g without the probiotic; femur weight: SZ 0.51g ±0.05g; GZ 0.52g±0.05g and 0.42g±0.03g without the probiotic). Femur zinc content was higher for zinc gluconate stabilized with glycine in the presence of the probiotic (97.04ppm±8.40ppm), and the results were similar for zinc sulfate with or without probiotic (84.51ppm±2.44ppm and 84.94ppm±2.28ppm, respectively). Serum antioxidant capacity and immune cellular response were also evaluated by using free radical scavenging assays and a T cell proliferation assay respectively. The free radical scavenging assay showed a tendency to increase with zinc provision, and the highest proliferation index was observed for glycine-stabilized zinc gluconate and the probiotic. Conclusion:The results indicate that the combination of zinc (as glycine-stabilized zinc gluconate) and a probiotic may be beneficial for the evaluated parameters.

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Plasmatic antioxidant capacity due to ascorbate using TEMPO scavenging and electron spin resonance

Cited by 10 publications

References 27 publications

Vitamin C prevents zidovudine-induced NAD(P)H oxidase activation and hypertension in the rat

Vitamin C prevents zidovudine-induced NAD(P)H oxidase activation and hypertension in the rat

High-throughput Total Cupric Ion Reducing Antioxidant Capacity of Biological Samples Determined Using Flow Injection Analysis and Microplate-based Methods

Glycine-Stabilized Zinc Gluconate Has Similar Bioavailability than Zinc Sulfate In a Zinc Fortified Probiotic Food

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