Plasmatic concentration of organochlorine lindane acts as metabolic disruptors in HepG2 liver cell line by inducing mitochondrial disorder

Benarbia, Mohamed El Amine; Macherel, David; Faure, Sébastien; Jacques, Caroline; Andriantsitohaina, Ramaroson; Malthièry, Yves

doi:10.1016/j.taap.2013.06.006

Cited by 21 publications

(9 citation statements)

References 44 publications

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“…The sustained activation of AMPK by lindane might be related to its actions on these signaling molecules. It might also be related to the reported actions of lindane on mitochondrial disorder and oxidative stress (Bagchi and Stohs, 1993;Benarbia et al, 2013), both of which are known to be able to activate AMPK (Cárdenas et al, 2010;Pung et al, 2013). This unexpected action of lindane on AMPK should not greatly affect our conclusion because a similar potentiating effect on AMPK activation was achieved by downregulation of Cx43 with siRNA or by using other hemichannel blockers.…”

Section: +mentioning

confidence: 62%

Purinergic control of AMPK activation by ATP released through connexin 43 hemichannels: pivotal roles in hemichannel-mediated cell injury

Chi

Gao

et al. 2014

Journal of Cell Science

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Connexin hemichannels regulate many cell functions. However, the molecular mechanisms involved remain elusive. Hemichannel opening causes loss of ATP, we therefore speculated a potential role for AMPK in the biological actions of hemichannels. , disclosure of hemichannels similarly enhanced AMPK activity, which protected cells from Cd 2+ -induced cell injury through suppression of mTOR. In summary, our data point to a channel-mediated mechanism for the regulation of AMPK through a purinergic signaling pathway. Furthermore, we define AMPK as a pivotal molecule that underlies the regulatory effects of hemichannels on cell survival.

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Section: +mentioning

confidence: 62%

Purinergic control of AMPK activation by ATP released through connexin 43 hemichannels: pivotal roles in hemichannel-mediated cell injury

Chi

Gao

et al. 2014

Journal of Cell Science

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“…ATP was quantified by high-performance liquid chromatography (HPLC) on 4.10 6 cells according to Benarbia Mel et al (2013).…”

Section: Nadh/nad + Ratio and Atp Measurementsmentioning

confidence: 99%

Assembly defects induce oxidative stress in inherited mitochondrial complex I deficiency

Leman

Guéguen

Desquiret-Dumas

et al. 2015

The International Journal of Biochemistry & Cell Biology

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“…Finalmente, um estudo recente conduzido por Benarbia et al 36 , realizado exclusivamente com o lindano (um isômero do HCH), procurou avaliar o mecanismo de desregulação endócrina provocado pelo composto. Os autores sugerem que a desregulação, de ordem metabólica, ocorra em nível mitocondrial, cuja função fica alterada, impactando no metabolismo energético celular.…”

Section: Discussionunclassified

Alterações nos marcadores hepáticos decorrentes da exposição ambiental a organoclorados no Brasil

Bahia

Guimarães²,

Asmus³

2014

Cad. saúde colet.

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Cad. Saúde Colet., 2014, Rio de Janeiro, 22 (2): 133-41 133 Artigo OriginalResumo Objetivos: Estimar a magnitude da associação entre a exposição a resíduos de pesticidas organoclorados (OC) e alterações de marcadores hepáticos. Método: Estudo seccional conduzido em população exposta a OC residente em Cidade dos Meninos, Duque de Caxias (RJ), Brasil (n=354). Foram calculadas as taxas de alterações dos marcadores hepáticos (Alanina aminotransferase (ALT), Aspartato aminotransferase (AST), fosfatase alcalina, gama glutamil transpeptidase (GGT) e albumina) e, em seguida, por meio de regressão logística binária não condicional, foi estimada a associação entre a presença ou não de alterações e os grupos de exposição, criados com base na exposição a alimentos, solo e água contaminados no local. Razões de chance (Odds ratio − OR) brutas e ajustadas para os grupos de exposição e para os fatores de confusão e seus respectivos intervalos de confiança (IC95%) foram obtidos. Resultados: Após ajuste pela regressão logística, os resultados do presente estudo apontam para nenhuma associação entre alterações em enzimas hepáticas e a exposição a OC. Conclusão: O achado sugere que o metabolismo que envolve a distribuição e a biotransformação de OC no organismo não envolve toxicidade direta ao fígado.Palavras-chave: inseticidas organoclorados; gastroenterologia; disruptores endócrinos; Brasil. AbstractObjectives: To estimate the magnitude of the association between exposure to organochlorine pesticide residues and alterations of liver markers. Methods: Cross-sectional study conducted in a population exposed to organochlorines resident of Cidade dos Meninos, Duque de Caxias (RJ), Brazil (n=354). Rates were calculated from changes in hepatic markers (Alanine aminotransferase (AST), Aspartate aminotransferase (AST), alkaline phosphatase, gamma glutamyl transpeptidade (GGT) and albumin) and then, using binary logistic regression non-conditional, was estimated the association between the presence or absence of changes and exposure groups, created based on the exposure to food contaminated soil and water on site. Odds ratios unadjusted and adjusted for exposure groups and for confounding factors and their confidence interval (95%CI) were obtained. Results: After adjustment by logistic regression, the results of this study indicate no association between changes in liver enzyme abnormalities and exposure to organochlorines. Conclusion: Finding suggests that the metabolism involving the distribution and biotransformation of organochlorine compounds in the body involves no direct toxicity to the liver.

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Plasmatic concentration of organochlorine lindane acts as metabolic disruptors in HepG2 liver cell line by inducing mitochondrial disorder

Cited by 21 publications

References 44 publications

Purinergic control of AMPK activation by ATP released through connexin 43 hemichannels: pivotal roles in hemichannel-mediated cell injury

Purinergic control of AMPK activation by ATP released through connexin 43 hemichannels: pivotal roles in hemichannel-mediated cell injury

Assembly defects induce oxidative stress in inherited mitochondrial complex I deficiency

Alterações nos marcadores hepáticos decorrentes da exposição ambiental a organoclorados no Brasil

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