Plasmid DNA studies in <i>Lactobacillus plantarum</i> strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)

Abstract

RESUMENEstudios de ADN plásmido en cepas de Lactobacillus plantarum aisladas de aceitunas fermentadas: La producción de y la inmunidad a plantaricina OL15 está asociada a un plásmido de 9.6 Kb (pOL15).En un estudio previo, se aislaron 12 cepas de Lactobacillus plantarum a partir de aceitunas fermentadas. Entre ellas, solo L. plantarum OL15 produjo bacteriocinas (plantaricin OL15). En este estudio, se examinó el contenido de AND plásmido en las 12 cepas citadas. Entre ellas, 9 cepas han mostrado de una a tres bandas de plásmido con tamaños en el rango de 5.4 a 12.2 kb. L. plantarum OL15 exhibió un plásmido (9.6 kb) que se denominó pOL15. Después del curado con novobiocina y bromuro de etidio, la pérdida del plásmido pOL15 asociada a la pérdida de su facultad para producir plantaricin OL15, sugiere que la producción de plantaricina OL15 está ligada al plásmido. La plantaricin OL15 no se inactivó por amilasa ni por lipasa sugiriendo que su actividad no es dependiente de la presencia de carbohidratos o lípidos. La plantaricina OL15 mostró actividad frente a bacterias lácticas de diferentes especies y también frente a residuos de aceitunas y bacterias fitopatogénicas, incluyendo Pseudomonas y Erwinia PALABRAS-CLAVE: Aceitunas -Agentes de curadoBacteriocinas -Lactobacillus plantarum -Plásmido. SUMMARYPlasmid DNA studies in Lactobacillus plantarum strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15).Previously 12 Lactobacillus plantarum strains were isolated from fermented olives. Among these, only L. plantarum OL15 produced bacteriocin (plantaricin OL15). In this study, the 12 strains were examined for plasmid DNA content. Of these, 9 strains have shown one to three plasmid bands ranging in size from 5.4 to 12.2 kb. L. plantarum OL15 exhibited one plasmid (9.6 kb) which was named pOL15. After curing with novobiocin and ethidium bromide, the plasmid profile analysis of non producing derivatives, showed that the 9.6 kb plasmid pOL15 harbored by the parental strain had been lost in all cases and none of them regained the ability to produce plantaricin OL15 suggesting that the production of plantaricin OL15 is plasmid linked. Plantaricin OL15 was not inactived by amylase and lipase suggesting that plantaricin OL15 activity was not dependent on the presence of either a carbohydrate or lipid moiety. Plantaricin OL15 showed activity against lactic acid bacteria Plasmid DNA studies in Lactobacillus plantarum strains isolated from olive fermentations: production of and immunity to plantaricin OL15 is associated to a 9.6 Kb plasmid (pOL15)

Technological and probiotic characteristics of Lactobacillus plantarum strains isolated from traditionally produced fermented vegetables

“…Similarly, Ruiz-Barba et al (1991) also showed that 35 L. plantarum strains isolated from different fermented green olive samples harboured large number of plasmids (from 5 to 16) ranging from 2 to 68 kb in size. A similarly wide variety of plasmid profiles in L. plantarum strains isolated from fermented olive samples was also reported by Mourad (2007).…”

In Silico Analysis of Plantaricin EF that Expressed by Plasmid-Associated Bacteriocin Production Gene of Lactobacillus plantarum IBL-2 for Anti-Candida Agent Potential

“…Plasmid DNA in the lactic acid bacteria is not always easily detected due to the copy number and isolation procedure (Mourad, 2007). In this study two kind of bacteriocin consist of plnB and plnEF were confirmed in the L. plantarum IBL-2 plasmid.…”