Plasmid Encoding Mirna-200C Delivered by Caco ₃ -Based Nanoparticles Enhances Rat Alveolar Bone Formation

Abstract

Aim: miRNAs have been shown to improve the restoration of craniofacial bone defects. This work aimed to enhance transfection efficiency and miR-200c-induced bone formation in alveolar bone defects via plasmid DNA encoding miR-200c delivery from CaCO3 nanoparticles. Materials & methods: The CaCO3/ miR-200c delivery system was evaluated in vitro (microscopy, transfection efficiency, biocompatibility) and miR-200c-induced in vivo alveolar bone formation was assessed via micro-computed tomography and histology. Results: CaCO3 nanoparticles significantly enhanced the transfection of plasmid DNA encoding miR-200c without inflammatory effects and sustained miR-200c expression. CaCO3/ miR-200c treatment in vivo significantly increased bone formation in rat alveolar bone defects. Conclusion: CaCO3 nanoparticles enhance miR-200c delivery to accelerate alveolar bone formation, thereby demonstrating the application of CaCO3/ miR-200c to craniofacial bone defects.

CaCO3Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma

“…Figures 5C and D Previous studies have reported that CaCO3-based nanoparticles can effectively improve transfection efficiencies of pDNA encoding miR-200c into human preosteoblasts (Remy et al 2022). Local application of collagen sponges incorporating CaCO3/pDNA miR-200c complexes effectively enhances bone formation in alveolar bone defects (Remy et al 2022). In the present studies, it is confirmed that the system of CaCO3 with PS co-precipitation nanoparticles effectively improves transfection efficiencies of pDNA encoding miR-200c into OSCC cells.…”

“…Quantitatively, GFP expression measured qRT-PCR was significantly higher in tumor tissue with miR-200c treatment than that of untreated controls (Figure 5B). Figures 5C and D Previous studies have reported that CaCO3-based nanoparticles can effectively improve transfection efficiencies of pDNA encoding miR-200c into human preosteoblasts (Remy et al 2022). Local application of collagen sponges incorporating CaCO3/pDNA miR-200c complexes effectively enhances bone formation in alveolar bone defects (Remy et al 2022).…”

“…pDNA encoding miR-200c, empty vector (EV), and scramble vector (SV) were prepared as in our previous studies (Hong et al 2016). CaCO3/pDNA miR-200c nanocomplexes at CaCO3:PS ratios of 1:0.03125, 1:0.125, 1:0.25, and 1:0.5 were prepared as previously described (Remy et al 2022). The nanocomplexes were visualized using scanning electron microscopy (SEM; Hitachi S-4800, Japan).…”

“…Notably, protamine sulfate (PS), a clinically used antidote for heparin-induced anticoagulation, has been reported to substantially improve the transfection efficiency of CaCO3-mediated plasmid DNA (pDNA) into cells (He et al 2018;Wang et al 2014). Our previous studies have demonstrated that pDNA miR-200c delivered by CaCO3 effectively improves transfection efficiency and alveolar bone formation (Remy et al 2022)…”

CaCO3 Nanoparticles Delivering MicroRNA-200c Suppress Oral Squamous Cell Carcinoma

“…Notably, protamine sulfate (PS), a clinically used antidote for heparin-induced anticoagulation, has been reported to substantially improve the transfection efficiency of CaCO 3 -mediated plasmid DNA (pDNA) into cells (Wang et al 2014; He et al 2018). Our previous studies have demonstrated that pDNA miR-200c delivered by CaCO 3 effectively improves transfection efficiency and alveolar bone formation (Remy et al 2022). CaCO 3 -based nanoparticles have also been developed for cancer therapy (Chen et al 2016; Poojari et al 2016).…”