Plasmid Fusion and Recombination Events That Occurred during Conjugation of
            <i>poxtA</i>
            -Carrying Plasmids in Enterococci

Shan, Xinxin; Yang, Mengyan; Wang, Nannan; Schwarz, Štefan; Li, Dexi; Du, Xiang-Dang

doi:10.1128/spectrum.01505-21

Cited by 12 publications

(8 citation statements)

References 39 publications

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“…It is reported that ISs participate in 63.2% of the transferred AMR genes between plasmids and chromosomes ( 24 ). For instance, IS 26 and IS 1216E (IS 6 family) could mediate plasmid fusion and transmit resistance-encoding genes ( 25 , 31 ). Besides, replicative transpositions were reported in other presumed MGEs, such as Tn 4401 (Tn 3 family), IS 903B (IS 5 family), and IS Kpn14 (IS 1 family) ( 32 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae

et al. 2022

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Nowadays, the underlying mobilization mechanism and evolutionary processes of nonconjugative virulence or resistance plasmids in Klebsiella pneumoniae remain poorly understood. Our study revealed the high conjugation ability of IncN3 plasmid isolated from carbapenem-resistant K. pneumoniae and confirmed its capability to mobilize the nonconjugative virulence or resistance plasmids.

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Section: Discussionmentioning

confidence: 99%

Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae

et al. 2022

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“…These strains were isolated from environmental (pF88_1) and human samples (p17-318_1 and pE843-TC-299). The fourth plasmid, pE843-171, is carried by Enterococcus lactis E843, and it is characterized as VSE-MDR (Shan et al, 2022 ). According to these results, pUC7251_1 holds unique traits, and although the prevalence of VSE-MDR is high, none of the results on BLAST showed VSE-MDR from food origin.…”

Section: Resultsmentioning

confidence: 99%

Genomic Insights of Enterococcus faecium UC7251, a Multi-Drug Resistant Strain From Ready-to-Eat Food, Highlight the Risk of Antimicrobial Resistance in the Food Chain

et al. 2022

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The presence of multi-drug resistant (MDR) bacteria in ready-to-eat foods comprises a threat for public health due to their ability to acquire and transfer antibiotic-resistant determinants that could settle in the microbiome of the human digestive tract. In this study, Enterococcus faecium UC7251 isolated from a fermented dry sausage was characterized phenotypically and genotypically to hold resistance to multiple antibiotics including aminoglycosides, macrolides, β-lactams, and tetracyclines. We further investigated this strain following a hybrid sequencing and assembly approach (short and long reads) and determined the presence of various mobile genetic elements (MGEs) responsible of horizontal gene transfer (HGT). On the chromosome of UC7251, we found one integrative and conjugative element (ICE) and a conjugative transposon Tn916-carrying tetracycline resistance. UC7251 carries two plasmids: one small plasmid harboring a rolling circle replication and one MDR megaplasmid. The latter was identified as mobilizable and containing a putative integrative and conjugative element-like region, prophage sequences, insertion sequences, heavy-metal resistance genes, and several antimicrobial resistance (AMR) genes, confirming the phenotypic resistance characteristics. The transmissibility potential of AMR markers was observed through mating experiments, where Tn916-carried tetracycline resistance was transferred at intra- and inter-species levels. This work highlights the significance of constant monitoring of products of animal origin, especially RTE foodstuffs, to stimulate the development of novel strategies in the race for constraining the spread of antibiotic resistance.

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“…Formation of larger cointegrates, likely with an involvement of very ubiquitous IS 1216 , might explain the existence of larger, approximately 170 kb plasmids hybridizing with the vanA probe, apart from the main 21.6 kb form of the vanA- plasmid. Formation of such fusions was indeed demonstrated for larger conjugative plasmids and smaller mobilizable poxtA- plasmids, enabling transfer of linezolid resistance from E. faecalis and Enterococcus lactis [ 62 ]. The majority of repUS1 pVEF1 - vanA plasmids represented multireplicons, which contained also rep18a , located upstream Tn 1546 and in some instances repUS1 pVEF1 - vanA plasmids carried also rep1, rep2 and/or rep9 .…”

Section: Discussionmentioning

confidence: 99%

VanA-Enterococcus faecalis in Poland: hospital population clonal structure and vanA mobilome

Wardal

Żabicka

Hryniewicz

et al. 2022

Eur J Clin Microbiol Infect Dis

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The aim of our study was to characterize the epidemiological situation concerning nosocomial vancomycin-resistant Enterococcus faecalis of VanA-phenotype (VREfs-VanA) in Poland by investigating their clonal relationships and the vanA-associated mobilome. One-hundred twenty-five clinical isolates of VREfs-VanA collected between 2004 and 2016 were studied by phenotypic assays, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), PCR detection of plasmid-specific genes, and Tn1546 structure and localization mapping. Selected isolates were subjected to PFGE-S1, Southern hybridization, genomic sequencing and conjugation experiments. The majority of isolates (97.6%) belonged to clonal complexes CC2 and CC87 of E. faecalis. All isolates were resistant to vancomycin and teicoplanin, and resistance to ciprofloxacin and aminoglycosides (high level) was very prevalent in this group. VanA phenotype was associated with 16 types of Tn1546, carrying insertion sequences IS1216, ISEfa4, IS1251 and IS1542, located on repUS1pVEF1, rep1pIP501, rep2pRE25, rep9pAD1/pTEF2/pCF10 and rep6pS86 replicons. The most common Tn1546 B- and BB-type transposons, harbouring one or two copies of IS1216, were inserted between rep18ap200B and repUS1pVEF1 genes and located on ~ 20 kb and 150–200 kb plasmids. VREfs-VanA in Poland represent a polyclonal group, indicating a number of acquisitions of the vanA determinant. The repUS1pVEF1-vanA plasmids, unique for Poland, were the main factor beyond the acquisition of vancomycin resistance by E. faecalis, circulating in Polish hospitals.

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Plasmid Fusion and Recombination Events That Occurred during Conjugation of poxtA -Carrying Plasmids in Enterococci

Cited by 12 publications

References 39 publications

Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae

Transmission of Nonconjugative Virulence or Resistance Plasmids Mediated by a Self-Transferable IncN3 Plasmid from Carbapenem-Resistant Klebsiella pneumoniae

Genomic Insights of Enterococcus faecium UC7251, a Multi-Drug Resistant Strain From Ready-to-Eat Food, Highlight the Risk of Antimicrobial Resistance in the Food Chain

VanA-Enterococcus faecalis in Poland: hospital population clonal structure and vanA mobilome

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