Plasmid Involvement in Parathion Hydrolysis by
            <i>Pseudomonas diminuta</i>

Serdar, Cüneyt M.; Gibson, David T.; Munnecke, Douglas M.; Lancaster, John H.

doi:10.1128/aem.44.1.246-249.1982

Cited by 215 publications

(73 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Coumaphos is degraded by the other microorganisms like Flavobacterium sp. (Sethunathan & Yoshida, 1973), P. diminuta (Serdar et al, 1982), and Enterobacter sp. B-14 (Singh et al, 2004), which were isolated for their ability to degrade other organophosphorus compounds.…”

Section: Coumaphosmentioning

confidence: 99%

Microbial degradation of organophosphorus compounds

Singh¹,

Walker²

2006

FEMS Microbiol Rev

987

524

View full text Add to dashboard Cite

Synthetic organophosphorus compounds are used as pesticides, plasticizers, air fuel ingredients and chemical warfare agents. Organophosphorus compounds are the most widely used insecticides, accounting for an estimated 34% of world-wide insecticide sales. Contamination of soil from pesticides as a result of their bulk handling at the farmyard or following application in the field or accidental release may lead occasionally to contamination of surface and ground water. Several reports suggest that a wide range of water and terrestrial ecosystems may be contaminated with organophosphorus compounds. These compounds possess high mammalian toxicity and it is therefore essential to remove them from the environments. In addition, about 200,000 metric tons of nerve (chemical warfare) agents have to be destroyed world-wide under Chemical Weapons Convention (1993). Bioremediation can offer an efficient and cheap option for decontamination of polluted ecosystems and destruction of nerve agents. The first micro-organism that could degrade organophosphorus compounds was isolated in 1973 and identified as Flavobacterium sp. Since then several bacterial and a few fungal species have been isolated which can degrade a wide range of organophosphorus compounds in liquid cultures and soil systems. The biochemistry of organophosphorus compound degradation by most of the bacteria seems to be identical, in which a structurally similar enzyme called organophosphate hydrolase or phosphotriesterase catalyzes the first step of the degradation. organophosphate hydrolase encoding gene opd (organophosphate degrading) gene has been isolated from geographically different regions and taxonomically different species. This gene has been sequenced, cloned in different organisms, and altered for better activity and stability. Recently, genes with similar function but different sequences have also been isolated and characterized. Engineered microorganisms have been tested for their ability to degrade different organophosphorus pollutants, including nerve agents. In this article, we review and propose pathways for degradation of some organophosphorus compounds by microorganisms. Isolation, characterization, utilization and manipulation of the major detoxifying enzymes and the molecular basis of degradation are discussed. The major achievements and technological advancements towards bioremediation of organophosphorus compounds, limitations of available technologies and future challenge are also discussed.

show abstract

Section: Coumaphosmentioning

confidence: 99%

Microbial degradation of organophosphorus compounds

Singh¹,

Walker²

2006

FEMS Microbiol Rev

987

524

View full text Add to dashboard Cite

show abstract

“…[47,48]). These include species of Flat'obacterium [49], Arthrobacter [50], and Pseudomonas [51][52][53][54], as well as many isolates which were not further identified [55][56][57]. In principle, parathion and malathion may also provide both sulfur and phosphorus for growth, but research into this aspect of organophosphate degradation has been much less extensive.…”

Section: Phosphorothionate Insecticidesparathion and Malathionmentioning

confidence: 99%

Microbial metabolism of sulfurand phosphorus-containing xenobiotics

Kertesz

Cook

Leisinger

1994

FEMS Microbiology Reviews

121

View full text Add to dashboard Cite

Abstract:The enzymes involved in the microbial metabolism of many important phosphorus-or sulfur-containing xenobiotics, including organophosphate insecticides and precursors to organosulfate and organosulfonate detergents and dyestuffs have been characterized. In several instances their genes have been cloned and analysed. For phosphonate xenobiotics, the enzyme system responsible for the cleavage of the carbon-phosphorus bond has not yet been observed in vilro, though much is understood on a genetic level about phosphonate degradation. Phosphonate metabolism is regulated as part of the Pho regulon, under phosphate starvation control. For organophosphorothionate pesticides the situation is not so clear, and the mode of regulation appears to depend on whether the compounds are utilized to provide phosphorus, carbon or sulfur for cell growth. The same is true for organosulfonate metabolism, where different (and differently regulated) enzymatic pathways are involved in the utilization of sulfonates as carbon and as sulfur sources, respectively. Observations at the protein level in a number of bacteria suggest that a regulatory system is present which responds to sulfate limitation and controls the synthesis of proteins involved in providing sulfur to the cell and which may reveal analogies between the regulation of phosphorus and sulfur metabolism.

show abstract

“…For some organophosphates such as parathion, it has been relatively easy to isolate degrading bacteria: two different strains, Flavobacterium sp. ATCC 27551 and Pseudomonas diminuta MG, have been isolated from soils in the Philippines and United States, respectively (Sethunathan & Yoshida, 1973;Serdar et al, 1982). In addition, studies on the degradation of methyl parathion also were made (Chaudhry et al, 1988;Rani & Lalithakumari, 1994).…”

Section: Introductionmentioning

confidence: 99%

Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil

Yang

Liu

Guo

et al. 2006

FEMS Microbiology Letters

200

151

View full text Add to dashboard Cite

An effective chlorpyrifos-degrading bacterium (named strain YC-1) was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. Based on the results of phenotypic features, phylogenetic similarity of 16S rRNA gene sequences and BIOLOG test, strain YC-1 was identified as the genus Stenotrophomonas. The isolate utilized chlorpyrifos as the sole source of carbon and phosphorus for its growth and hydrolyzed chlorpyrifos to 3,5,6-trichloro-2-pyridinol. Parathion, methyl parathion, and fenitrothion also could be degraded by strain YC-1 when provided as the sole source of carbon and phosphorus. The gene encoding the organophosphorus hydrolase was cloned using a PCR cloning strategy based on the known methyl parathion degrading (mpd) gene of Plesiomonas sp. M6. Sequence blast result indicated this gene has 99% similar to mpd. The inoculation of strain YC-1 (10(6) cells g(-1)) to soil treated with 100 mg kg(-1) chlorpyrifos resulted in a higher degradation rate than in noninoculated soils. Theses results highlight the potential of this bacterium to be used in the cleanup of contaminated pesticide waste in the environment.

show abstract

Plasmid Involvement in Parathion Hydrolysis by Pseudomonas diminuta

Cited by 215 publications

References 24 publications

Microbial degradation of organophosphorus compounds

Microbial degradation of organophosphorus compounds

Microbial metabolism of sulfurand phosphorus-containing xenobiotics

Cloning of mpd gene from a chlorpyrifos-degrading bacterium and use of this strain in bioremediation of contaminated soil

Contact Info

Product

Resources

About