Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients

Beige, Fahimeh; Salehi, Majid Baseri; Bahador, Nima; Mobasherzadeh, Sina

doi:10.5812/jjm.13567

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…The results obtained illustrated that ceftazidime, meropenem, cefotaxime, ceftazidine and amikacin resistant marker genes in clinical isolated A. baumannii were plasmid mediated. Transfer of antibiotic resistant genes by plasmids to other nosocomial pathogens can create complications in the treatment of patients (Beige et al, 2015). Therefore, the existence of A. baumannii in the hospital, especially in the ICU might be encountered as a major problem.…”

Section: Discussionmentioning

confidence: 99%

“…Recently multidrug resistance character of Acinetobacter considered as a major problem for commercially available antibiotic (Bergogne-Berezin, 2007;Dijkshoorn et al, 2007). Among all species of Acinetobacter, A. baumannii has become a significant pathogen, especially in the intensive care unit (Bauer et al, 1966;Brisson-Noel et al, 1988;Courvalin, 1994;Beige et al, 2015). Survival of this bacterium in the hospital, especially in the ICU seems to be related to their ability of acquiring antibiotic resistant genes.…”

Section: Discussionmentioning

confidence: 99%

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Plasmid Curing Assay in Clinical Isolates of Antibiotic Resistant Acinetobacter baumannii

Dasmeh¹,

Baserisale²,

Emami³

2015

Microbiology J.

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Acinetobacter baumannii an antibiotic resistant bacterium was isolated and identified from clinical samples and assessed for plasmid carrying ability and the locations of resistant gene markers. A total of hundred and sixty six clinical samples were collected from the respiratory tracts and wound infections in the patients hospitalized in the Intensive Care Unit (ICU) of Namaze Hospital, Iran. The isolates were identified into phenotypes and genotypes and their antibiotic susceptibility assessed by disk diffusion method. The antibiotics used were: amikacin 30 µg, gentamicin 10 µg, imepenem 10 µg, meropenem 10 µg, ceftazidime 30 µg, ceftaxime 30 µg, ciprofloxacin 5 µg, polymyxin 300B and tetracycline 30 µg. The resistance strains of Acinetobacter baumannii was subjected to plasmid curing to find plasmid mediated resistance markers. The results showed that fifty one Acinetobacter spp. were isolated and all of them were identified as A. baumannii. In addition, the isolates were resistant to cefotaxime (100%) and gentamicin (90.1%) and susceptible to polymyxin (100%). The results from plasmid curing showed that the acridine orange could cure, the plasmids of A. baumannii cured. A. baumannii isolates were sensitive to ceftazidime, meropenem, cefotaxime, ceftazidine and amikacin. Therefore, antibiotic resistance markers associated with these antibiotics were plasmid encoded. The present study showed that A. baumannii is an important nosocomial infection in the ICU. Many antibiotic resistance markers in the A. baumannii were carried by the plasmids. Hence, they might be transmitted easily among the bacteria in the hospitals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Plasmid Curing Assay in Clinical Isolates of Antibiotic Resistant Acinetobacter baumannii

Dasmeh¹,

Baserisale²,

Emami³

2015

Microbiology J.

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“…Klebsiella pneumoniae belongs to the Enterobacteriaceae family and is described as a gram-negative, encapsulated, and lactose-fermenting bacteria (2) that is responsible for various hospital-acquired infections such as pneumonia, septicemia, diarrhea, liver abscess, endophthalmitis, meningitis, urinary tract infections, and bacteremia, whose mortality rates are high (3). Along with other gram-negative bacteria such as Pseudomonas aeruginosa and Acinetobacter baumannii, K. pneumonia pathogens are commonly isolated from patients with burn infections (4). Most of such infections are caused by multidrug-resistant (MDR) strains that interrupt the treatment processes (5).…”

Section: Introductionmentioning

confidence: 99%

Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran

Vaziri

Afsharian

Mansouri

et al. 2020

Jundishapur J Microbiol

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Background: Assessment of bacteria such as Klebsiella pneumonia has shown that Plasmid-mediated quinolone resistance (PMQR) affects antibiotics resistance (e.g., quinolones). Objectives: We studied the prevalence of qnr and aac(6’)Ib-cr genes in extended-spectrum beta-lactamase (ESBL)-producing K. pneumonia strains isolated from burn wounds of patients in the city of Kermanshah, Iran. Methods: This descriptive-analytical study was conducted on 126 K. pneumonia strains isolated collected from burn wounds. Biochemical tests were used to detect the strains. The frequency of the ESBL-producing isolates was determined by phenotypic tests of the combination disk (CD) method after determining the antibiotic susceptibility pattern of the isolates through the Kirby-Bauer disc diffusion test. The prevalence of the qnr and aac(6’)-Ib-cr genes was determined using their special primers as well as polymerase chain reaction (PCR). Results: Of the 126 K. pneumonia isolates, 52 (41.3%) were identified as ESBL-producing strains. ESBL-producing isolates showed higher resistance against antibiotics than non-ESBL-producing ones. PMQR relevance and resistance to ciprofloxacin were, respectively, determined at 80.76% and 59.6%. The most frequent gene was aac(6’)-Ib-cr (n = 70, 55.6%), followed by the qnrB (n = 44, 34.9%. Conclusions: This study showed a high prevalence of qnr genes in ESBL-producing K. pneumonia isolates and antibiotic resistance. Given the horizontal transmission of antibiotic resistance genes among bacteria by mobile genetic elements, timely identification of infections caused by ESBL-producing and antimicrobial-resistant K. pneumonia strains is of paramount importance.

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“…That was found by previous studies the mechanisms of antibiotic resistance associated with clinical XDR-PA isolates are complex [ 6 , 7 ]. The prevailing hypothesis is that XDR-PA isolates acquire numerous drug-resistance determinants through horizontal gene transfer that is mediated by mobile genetic elements [ 8 – 10 ]. In addition, genes encoding the antibiotic-inactivating enzymes β-lactamases, aminoglycoside-modifying enzymes (AMEs), and 16S rRNA methylases (16S-RMTases) are frequently associated with antibiotic resistance in XDR-PA strains.…”

Section: Introductionmentioning

confidence: 99%

Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China

Zou

Dou

et al. 2016

Ann Clin Microbiol Antimicrob

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BackgroundPseudomonas aeruginosa strains that are classed as extensively drug resistant (XDR-PA) are resistant to all antibiotics except for one or two classes and are frequently the cause of hard-to-treat infections worldwide. Our study aimed to characterize clinical XDR-PA isolates recovered during 2011–2012 at nine hospitals in the Hunan province of China.MethodsThirty-seven non-repetitive XDR-PA strains from 37 patients were investigated for genes encoding antimicrobial resistance determinants, efflux pumps, outer membrane proteins, and movable genetic elements using polymerase chain reaction (PCR). The expression of genes encoding the efflux pump component MexA and the outer membrane protein OprD was measured using real-time PCR. In addition, clonal relatedness of these XDR-PA isolates was analyzed by pulsed-field gel electrophoresis (PFGE).ResultsVarious genes encoding antimicrobial resistance determinants were found in all isolates. In particular, the blaTEM-1, blaCARB, armA, blaIMP-4, blaVIM-2, and rmtB, were found in 100, 37.8, 22, 22, 19 and 5 % of the isolates, respectively. Remarkably, two isolates coharbored blaIMP-4, blaVIM-2, and armA. In all 37 antibiotic-resistant strains, the relative expression of oprD was decreased while mexA was increased compared to the expression of these genes in antibiotic-susceptible P. aeruginosa strains. All of the XDR-PA isolates harbored class I integrons as well as multiple other mobile genetic elements, such as tnpU, tnp513, tnpA (Tn21), and merA. A high genotypic diversity among the strains was detected by PFGE.ConclusionsMultiple antibiotic-resistance mechanisms contributed to the drug resistance of the XDR-PA isolates investigated in this study. Thus, the XDR-PA isolates in this area were not clonally related. Instead, multiple types of movable genetic elements were coharbored within each XDR-PA isolate, which may have aided the rapid development of these XDR-PA strains. This is the first report of XDR-PA strains that coharbor blaIMP-4, blaVIM-2, and armA.

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Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients

Cited by 13 publications

References 22 publications

Plasmid Curing Assay in Clinical Isolates of Antibiotic Resistant Acinetobacter baumannii

Plasmid Curing Assay in Clinical Isolates of Antibiotic Resistant Acinetobacter baumannii

Frequency of qnr and aac(6’)Ib-cr Genes Among ESBL-Producing Klebsiella pneumoniae Strains Isolated from Burn Patients in Kermanshah, Iran

Characterization of clinical extensively drug-resistant Pseudomonas aeruginosa in the Hunan province of China

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