Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula

Sonnevend, Ágnes; Ghazawi, Akela; AlQahtani, Manaf; Shibl, Atef M.; Jamal, W.; Hashmey, Rayhan; Pal, Tibor

doi:10.1016/j.ijid.2016.07.007

Cited by 74 publications

(68 citation statements)

References 26 publications

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“…KX013538), isolated from a pathogenic human E . coli in the United Arab Emirates [31], and pHNSHP45 (KP347127) the first reported pMCR [1]. The other 2 pMCRs (from M19736 and M19855) were more similar to plasmid pECJS-61-63 (KX084393) from an E .…”

Section: Resultsmentioning

confidence: 99%

Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

et al. 2017

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We have characterized nine mcr-1-harboring plasmids from clinical Escherichia coli isolates previously described in Argentina and Canada. Three of these plasmids carried a mcr-1-variant called here mcr-1.5. All these E. coli isolates were not clonally related and were recovered in different years and locations. However, their mcr-1-harboring plasmids showed high identity among them and to others characterized in other countries, which strongly suggests that this plasmid-type is playing an important role in spreading this mechanism of resistance to polymyxins.

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Section: Resultsmentioning

confidence: 99%

Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

et al. 2017

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“…Furthermore, a recent study by Sonnevend et al also showed that mcr-1 is present in Bahrain, where it was detected in two E. coli isolates cultured from human clinical samples (23). In silico MLST assigned the isolate to ST-617, a member of the globally distributed clonal complex 10 (CC10) and part of phylogenetic group A, which is most often associated with commensal E.…”

Section: Discussionmentioning

confidence: 97%

“…Though the E. coli strains in the study of Sonnevend et al belonged to different STs (ST-648 and ST-224), both carried mcr-1 on an IncI2 plasmid (23). …”

Section: Discussionmentioning

confidence: 99%

Analysis of Serial Isolates of mcr-1 -Positive Escherichia coli Reveals a Highly Active IS Apl1 Transposon

Snesrud

Ong

Corey

et al. 2017

Antimicrob Agents Chemother

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The emergence of a transferable colistin resistance gene (mcr-1) is of global concern. The insertion sequence ISApl1 is a key component in the mobilization of this gene, but its role remains poorly understood. Six Escherichia coli isolates were cultured from the same patient over the course of 1 month in Germany and the United States after a brief hospitalization in Bahrain for an unconnected illness. Four carried mcr-1 as determined by real-time PCR, but two were negative. Two additional mcr-1-negative E. coli isolates were collected during follow-up surveillance 9 months later. All isolates were analyzed by whole-genome sequencing (WGS). WGS revealed that the six initial isolates were composed of two distinct strains: an initial ST-617 E. coli strain harboring mcr-1 and a second, unrelated, mcr-1-negative ST-32 E. coli strain that emerged 2 weeks after hospitalization. Follow-up swabs taken 9 months later were negative for the ST-617 strain, but the mcr-1-negative ST-32 strain was still present. mcr-1 was associated with a single copy of ISApl1, located on a 64.5-kb IncI2 plasmid that shared >95% homology with other mcr-1 IncI2 plasmids. ISApl1 copy numbers ranged from 2 for the first isolate to 6 for the final isolate, but ISApl1 movement was independent of mcr-1. Some movement was accompanied by gene disruption, including the loss of genes encoding proteins involved in stress responses, arginine catabolism, and l-arabinose utilization. These data represent the first comprehensive analysis of ISApl1 movement in serial clinical isolates and reveal that, under certain conditions, ISApl1 is a highly active IS element whose movement may be detrimental to the host cell.

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“…Since the discovery of the colistin resistance gene mcr-1, an increasing number of plasmids carrying this resistance determinant have been reported, among which the ϳ60-kb IncI2 plasmid was one of the three common types of conjugative mcr-1-bearing plasmids (1,2). This IncI2 plasmid has been reported to carry an additional bla CTX-M-55 or bla CTX-M-64 gene (2,3). In this study, we report the recovery of a similar IncI2-type mcr-1-bearing conjugative plasmid that harbors a novel variant of bla CTX-M-64 , which encodes resistance to class A ␤-lactamase inhibitors.…”

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confidence: 99%

Genetic and Functional Characterization of bla _CTX-M-199 , a Novel Tazobactam and Sulbactam Resistance-Encoding Gene Located in a Conjugative mcr-1 -Bearing IncI2 Plasmid

Cai

Cheng

Shen

et al. 2017

Antimicrob Agents Chemother

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Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula

Cited by 74 publications

References 26 publications

Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

Analysis of Serial Isolates of mcr-1 -Positive Escherichia coli Reveals a Highly Active IS Apl1 Transposon

Genetic and Functional Characterization of bla _CTX-M-199 , a Novel Tazobactam and Sulbactam Resistance-Encoding Gene Located in a Conjugative mcr-1 -Bearing IncI2 Plasmid

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Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula

Cited by 74 publications

References 26 publications

Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

Molecular characteristics of mcr-1-carrying plasmids and new mcr-1 variant recovered from polyclonal clinical Escherichia coli from Argentina and Canada

Analysis of Serial Isolates of mcr-1 -Positive Escherichia coli Reveals a Highly Active IS Apl1 Transposon

Genetic and Functional Characterization of bla CTX-M-199 , a Novel Tazobactam and Sulbactam Resistance-Encoding Gene Located in a Conjugative mcr-1 -Bearing IncI2 Plasmid

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Genetic and Functional Characterization of bla _CTX-M-199 , a Novel Tazobactam and Sulbactam Resistance-Encoding Gene Located in a Conjugative mcr-1 -Bearing IncI2 Plasmid