Plasmid-mediated transfer of nitrogen-fixing capability to bacteria from the rhizosphere of grasses

Kleeberger, A.; Klingmüller, Walter

doi:10.1007/bf00268070

Cited by 23 publications

(3 citation statements)

References 14 publications

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“…They reported increased virulence in some transconjugants but provided no clear evidence that nif expression was responsible. Kleeberger & Klingmuller (1980) transferred pRDl to a strain of cloacae isolated from grass roots, with the possibility of generating a diazotrophic root association. They obtained Nif+ transconjugants despite the fact that some transconjugants lost nif and, as in the earliest transfers to E. coli, some derivatives integrated nif into the E. cloacae chromosome and showed promising stability as regards NiU even in association with grass roots.…”

Section: Kp Nif In Other Bacteriamentioning

confidence: 99%

nifgenes in alien backgrounds

Postgate

Dixon

Hill

et al. 1987

Phil. Trans. R. Soc. Lond. B

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Since the original construction of diazotrophic Escherichia coli by conjugal transfer of nif genes from Klebsiella pneumoniae in 1972, the manipulation of nif genes into alien prokaryotic backgrounds has become routine: much of the basic genetics of the K. pneumoniae nif cluster was elucidated in an E. coli background. Gene transfers to new species and genera can give new information regarding the stability of nif genes and, particularly, conditions for their expression; recipients in which nif is partly expressed, or not expressed at all, are especially useful. Appropriate examples are discussed. New diazotrophic prokaryotes show little promise for practical exploitation but their construction should give forewarning of problems to be expected in the construction of diazotrophic eukaryotes, as well as hints concerning the ecology and evolution of diazotrophy.

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Section: Kp Nif In Other Bacteriamentioning

confidence: 99%

nifgenes in alien backgrounds

Postgate

Dixon

Hill

et al. 1987

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

show abstract

“…The expression of pRD1 markers in cultures in which the plasmid could not be detected by agarose gel electrophoresis was also observed in Enterobacter cloacae (5). Although no further experiments were performed, it was speculated that pRD1 had integrated into the E. cloacae chromosome.…”

mentioning

confidence: 98%

“…Plasmid pRD1 (formerly called RP41) was constructed from IncP-1 antibiotic resistance plasmid RP4 and contains the closely linked histidine and nitrogen fixation (nif) complexes from Klebsiella pneumoniae in addition to resistance to kanamycin (Kmr), tetracycline (Tc9, and ampicillin (Ap9 (3). pRD1 has been conjugatively transferred to a limited number of bacterial genera by selection for histidine complementation in the transconjugants (3,5,8). Among pseudomonads, pRD1 has been conjugatively transferred only to Pseudomonas fluorescens (8).…”

mentioning

confidence: 99%