Plasmid Molecular Biology

doi:10.1007/0-387-31489-x_13

Cited by 2 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA segregation in bacteria has been extensively studied using plasmids as a model system. Plasmids are extrachromosomal self-replicating species of DNA that usually encode genes for antibiotic resistance, production of bacteriocins, resistance to heavy metals, ultraviolet light, pathogen virulence factors and many other metabolic functions (Birge, 2006 ). Plasmids generally vary in size from a few kilobases to hundreds of kilobases, and their geometry is commonly circular or linear.…”

Section: Introductionmentioning

confidence: 99%

“…High copy number plasmids are generally small and replicate randomly during the cell cycle. These plasmids can reach up to a 100 copies per cell, and thus random assortment and segregation during cytokinesis can ensure sufficient distribution of these plasmids into two daughter cells (Birge, 2006 ; Million-Weaver and Camps, 2014 ). On the contrary, low copy number plasmids with <15 copies per cell cannot solely rely on random distribution for maintenance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Catching a Walker in the Act—DNA Partitioning by ParA Family of Proteins

Mishra

Srinivasan

2022

Front. Microbiol.

View full text Add to dashboard Cite

Partitioning the replicated genetic material is a crucial process in the cell cycle program of any life form. In bacteria, many plasmids utilize cytoskeletal proteins that include ParM and TubZ, the ancestors of the eukaryotic actin and tubulin, respectively, to segregate the plasmids into the daughter cells. Another distinct class of cytoskeletal proteins, known as the Walker A type Cytoskeletal ATPases (WACA), is unique to Bacteria and Archaea. ParA, a WACA family protein, is involved in DNA partitioning and is more widespread. A centromere-like sequence parS, in the DNA is bound by ParB, an adaptor protein with CTPase activity to form the segregation complex. The ParA ATPase, interacts with the segregation complex and partitions the DNA into the daughter cells. Furthermore, the Walker A motif-containing ParA superfamily of proteins is associated with a diverse set of functions ranging from DNA segregation to cell division, cell polarity, chemotaxis cluster assembly, cellulose biosynthesis and carboxysome maintenance. Unifying principles underlying the varied range of cellular roles in which the ParA superfamily of proteins function are outlined. Here, we provide an overview of the recent findings on the structure and function of the ParB adaptor protein and review the current models and mechanisms by which the ParA family of proteins function in the partitioning of the replicated DNA into the newly born daughter cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%