Plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from Nigeria

Olukoya, D. K.; Oni, OO

doi:10.1017/s0950268800047646

Cited by 34 publications

(32 citation statements)

References 14 publications

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“…Drug susceptibility of Salmonella and Shigella isolates showed that they were resistant to ampicillin, which is in agreement with the findings of other researchers in Nigeria [25], [26] and also in agreement with studies done in Kenya [27] and in Uganda [28]. In this study, Salmonella and Shigella were sensitive to the following antibiotics: ceftriaxone, gentamycin, ciprofloxacin, chloramphenicol, and nitrofurantoin.…”

Section: Discussionsupporting

confidence: 93%

Prevalence of enteric pathogens among patients with gastrointestinal presentations in the Lagos University Teaching Hospital (LUTH), Idi-Araba Lagos Nigeria

Ike¹,

Damola²

2014

IOSRJPBS

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Section: Discussionsupporting

confidence: 93%

Prevalence of enteric pathogens among patients with gastrointestinal presentations in the Lagos University Teaching Hospital (LUTH), Idi-Araba Lagos Nigeria

Ike¹,

Damola²

2014

IOSRJPBS

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“…Representative profiles of plasmids of each of the four serogroups are presented in Figures 1-4. DISCUSSION The shigellae continue to be important aetiological agents of dysentery gastroenteritis in India [1,11,12], the United States [13], Nigeria [2], Australia [3], Vellore [14] and Bangladesh [15]. This is also true for Taiwan [4,10].…”

Section: Resultsmentioning

confidence: 99%

“…Shigellosis is one of the most prevalent and important diseases in the developing countries [1][2][3], and is the major cause of dysentery in Taiwan [4]. Resistance of Shigella spp.…”

Section: Introductionmentioning

confidence: 99%

Drug resistance and plasmid profile of shigellae in Taiwan

Lin

Chang

1992

Epidemiol. Infect.

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SUMMARYOne hundred and twenty-eight shigella strains isolated from newborn and infant human faecal specimens at Kaohsiung Medical College Hospital in Taiwan were serogrouped, serotyped and examined for drug-resistance patterns and for the presence of plasmids. Forty-seven per cent of the isolates were found to belong to the Shigella sonnei serogroup, 41 % to the S. fiexneri group, 9 % to the S. boydii group and 3 % to the S. dysenteriae group. The serotype with the greatest number of strains was S. sonnei I. (29 %) followed by S. flexneri 1 (27%). Each strain was tested for resistance to 11 antimicrobial agents. Eighty-eight per cent of the strains were resistant to tetracycline, 87 % to chloramphenicol, 84 % to streptomycin, 52% to ampicillin, 25 % to nalidixic acid, 29% to kanamycin, 11 % to cephalothin, 11 % to neomycin, 10 % to cotrimoxazole, 1 % to amikacin and none to gentamicin. The most prevalent resistance pattern was Ap Cm Sm Tc (28%). Clinical isolates demonstrating multiple resistance were found to harbour a large transmissible plasmid of 45-75 MDa while isolates without multiple resistance did not. Two large virulence plasmids of 123 and 110 MDa were found in 12 strains of S. flexneri and 4 strains of S. sonnei phase I. Small plasmids of 4-5, 4 2, 3 5, 2 8, 2-5. 2 0 and 1P5 MDa were also present in all strains. These small plasmids were species specific and can be used as marker plasmids to identify species.

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“…Plasmid profile analysis could differentiate these selected isolates. This may reflect the presence of a large number of different endemic isolates or a high rate of plasmid transfer amongst isolates or both (Olukoya and Oni, 1990). The diverse patterns of plasmid profiles observed among isolates in the current study could be also due to higher exposure of people to enteric bacteria in these places with poor sanitation (Olukoya and Oni, 1990).…”

Section: Plasmid Profile Analysis-based Characterization Of Mdr Isolatesmentioning

confidence: 74%

Prevalence, Antimicrobial Susceptibilities and Molecular Characterization of Enteric Bacterial Pathogens Isolated from Patients with Infectious Diarrhoea in Cairo, Egypt

El-Moghazy¹,

Tawfick²,

El-Habibi³

2016

Int.J.Curr.Microbiol.App.Sci

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Infectious diarrhoea continues to cause significant morbidity and mortality, particularly among children worldwide. This problem is especially acute in developing countries. This study aimed to determine the prevalence, antimicrobial susceptibilities and plasmid-profile based molecular characterization of enteric bacteria isolated from patients with infectious diarrhoea in Cairo, Egypt. A total of 465 bacterial isolates were isolated from 412 diarrhoea faecal specimens and swabs collected from children and adults patients presenting acute diarrhoea, during the period from December 2013 to December 2015, at three hospitals in Cairo, Egypt. The fecal specimens were cultured by conventional methods for enteric bacterial pathogens. The biochemically identified bacterial isolates were subjected to the antimicrobial susceptibility testing using Kirby-Bauer disc diffusion method. For multi-drug resistant (MDR) isolates, identification and antimicrobial susceptibility pattern was confirmed by the automated MicroScan Walkaway system. Plasmid DNA was isolated from MDR isolates and different profiles were assessed by agarose gel electrophoresis. It was found that E. coli represents the most prevalent bacterial species among isolates (46.7 %), followed by K. pnemoniae (29.9 %), P. vulgaris (14.3 %), Enterobacter spp. (5.3 %), P. mirabilis (4.4 %), Citrobacter spp. (1.7 %) and Shigella spp. (0.7 %). Isolates in this study showed high levels of multidrug antimicrobial resistance to the antimicrobials tested as 46.9 % of isolates were MDR. The plasmid profile analysis revealed that plasmids were diverse and distributive among isolates, thus different patterns were detected. In conclusion, this study revealed that infectious diarrhoea is attributed to different bacterial species, with E. coli is the most prevalent one. Most of isolated enteric bacterial pathogens are MDR, which could be explained by the indiscriminate and widespread use of antimicrobial agents and the empirical antibiotic therapy in hospitals. The diverse patterns of plasmid profiles observed could be due to higher exposure of people to enteric bacterial pathogens in these places. Thus, regular surveillance for the etiological agents of infectious diarrhoea to monitor the changing epidemiology of these resistant bacteria is warranted. K e y w o r d sEnteric bacteria, Antimicrobial resistance, Infectious diarrhoea, Plasmid profile.

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Plasmid profile analysis and antimicrobial susceptibility patterns of shigella isolates from Nigeria

Cited by 34 publications

References 14 publications

Prevalence of enteric pathogens among patients with gastrointestinal presentations in the Lagos University Teaching Hospital (LUTH), Idi-Araba Lagos Nigeria

Prevalence of enteric pathogens among patients with gastrointestinal presentations in the Lagos University Teaching Hospital (LUTH), Idi-Araba Lagos Nigeria

Drug resistance and plasmid profile of shigellae in Taiwan

Prevalence, Antimicrobial Susceptibilities and Molecular Characterization of Enteric Bacterial Pathogens Isolated from Patients with Infectious Diarrhoea in Cairo, Egypt

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