Plasmid profile of Escherichia coli 0157:H7 from apparently healthy animals

Smith, SI; Aboaba, Olusimbo O.; Odeigha, P; Shodipo, K.; Adeyeye, JA; Ibrahim, A; Adebiyi, T; Onibokun, H.; Odunukwe, N N

doi:10.5897/ajb2003.000-1066

Cited by 29 publications

(28 citation statements)

References 7 publications

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“…Umolu et al 27 reported that E. coli Isolates with high multi-drug resistance profiles were found to possess multiple plasmids with large sizes in the range of 6.557 -23.130kb. This is also similar to what was observed by Smith et al 57 who reported that 47 of the E. coli isolated from animals in Lagos harboure detectable plasmids which ranged in sizes from 0.564kb to >23kb. Danbara et al 23 also reported plasmids of sizes between 3.9kb and 50kb in E. coli strains isolated from Traveller's diarrhoea.…”

supporting

confidence: 79%

Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Akingbade¹,

Balogun²,

Ojo³

et al. 2015

Afr H. Sci.

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Background: Multi-drug resistant Escherichia coli has become a major threat and cause of many urinary tract infections (UTIs) in Abeokuta, Nigeria. Objectives: This study was carried out to determine the resistant plasmids of multidrug resistant Escherichia coli isolated from (Urinary tract infections)UTIs in Abeokuta. Methods: A total of 120 Escherichia coli isolates were obtained from urine samples collected from patients attending inpatient and outpatient clinics presenting UTI; with their biodata. Antibiotics susceptibility was performed and multi-drug resistant isolates were selected for plasmid profiling. Plasmids were extracted by the alkaline lysis method, electrophoresed on 0.8% agarose gel and profiled using a gel-photo documentation system gel. Results: Escherichia coli isolates obtained shows high resistance to cloxacillin (92.5%), amoxicillin (90.8%), ampicillin (90.8%), erythromycin (75.8%), cotrimoxazole (70.0%), streptomycin (70.0%) and tetracycline (68.3%) while 85.8% and 84.2% were susceptible to gentamycin and ceftazidime respectively. Sixteen Escherichia coli strains were observed to be resistant to more than two classes of antibiotics. The resistant plasmid DNA was detectable in 6(37.5%) of the 16 multidrug resistant Escherichia coli having single sized plasmids of the same weight 854bp and were all resistant to erythromycin, cefuroxime, cloxacillin, amoxicillin, ampicillin and cotrimoxazole. Conclusion: This study has highlighted the emergence of multidrug resistant R-plasmids among Escherichia coli causing urinary tract infections in Abeokuta, Nigeria. There is a high level of resistance to many antimicrobials that are frequently used in Abeokuta, Nigeria.

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supporting

confidence: 79%

Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Akingbade¹,

Balogun²,

Ojo³

et al. 2015

Afr H. Sci.

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“…The observed resistance in these strains may drive from antimicrobial selection pressure as a result of indiscriminate use of antimicrobials in livestock production in Nigeria (Smith et al 2003; or as a result of intrinsic resistance in these environmental strains. According to Martinez et al (2009), antibiotics have the function of inhibiting competitors in natural environments, hence the proposition that antibiotic resistance genes evolved to protect bacteria in natural ecosystems from the toxicity of such naturally occurring antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Environmental impact assessment of Attenda abattoir, Ogbomoso southwestern Nigeria on surface and groundwater quality using geo-electrical imaging and microbiological analysis

Adelowo

Akinlabi

Fagade

2011

Environ Monit Assess

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The impact of Attenda abattoir, Ogbomoso southwestern Nigeria on four water sources was investigated using geoelectrical imaging and microbiological analysis. 2D electrical resistivity imaging indicated groundwater contamination by leachate from the abattoir. Electrical resistivity values measured by the traverse run directly on the waste dump and other areas impacted by the waste from the abattoir are generally very low (6.68-16.7 Ωm) in comparison to other positions (135-288 Ωm). The total viable bacteria count of the water samples ranged from 0.49 × 10(6) to 2.85 × 10(8) cfu/ml and all samples are contaminated with coliforms with the most probable number (MPN)/100 ml ranging from 110 to ≥ 1,600 MPN/100 ml. Among bacteria isolated from the study site (n = 95), resistance to eight antibiotics ranged from 35.8% to 94%. In addition, 85% of Streptococcus agalactiae (n = 31) and Staphylococci (n = 9) showed haemolytic activity while 92% of all isolates showed β-lactamase activity. These results suggest that operations of the abattoir may impact negatively on surrounding aquatic ecosystem and endanger the health of surrounding residents who use water from the wells for domestic purposes. Furthermore, such aquatic ecosystems may serve as reservoir of antibiotic resistant bacteria.

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“…Therefore, absence of 60-MDa plasmid in other strains in this study does not confirm whether those are non-enterohaemorrhagic E. coli. Based on our observations, the isolates containing plasmids of large molecular weights are potential members of EHEC and they may be virulenceassociated as well as toxigenic 11 . Association of plasmid DNA with antimicrobial resistance of E. coli as well as EHEC was described earlier 16 .…”

Section: Multidrug-resistant (Mdr) Patternmentioning

confidence: 86%

“…Association of plasmid DNA with antimicrobial resistance of E. coli as well as EHEC was described earlier 16 . Particularly, implication of large plasmid with multiple-drug resistance was broadly highlighted 11 . In the present study, we have successfully isolated nine large-plasmid containing strains, which showed remarkable resistance to a panel of carefully chosen antibiotics.…”

Section: Multidrug-resistant (Mdr) Patternmentioning

confidence: 99%

“…Apart from these virulence factors, most EHEC strains carry a large plasmid (approximately 60-MDa) that consists of hly EHEC gene involved in haemolytic activity through expression of pore-forming cytolysin, called haemolysin 5,10 . Presence of other large molecularweight plasmids implicated in pathogenesis has also been detected in EHEC serotypes 11 . Although most STEC serotypes as well as STEC O157 possess similar virulence properties that include shiga-toxin producing gene(s), eae gene and 60-MDa plasmid DNA, they have proven to be different in terms of other metabolic and growth characteristics.…”

mentioning

confidence: 96%

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Multidrug Resistance in Large-Plasmid-Associated Presumptive Enterohaemorrhagic Escherichia coli Isolated from Contaminated Lake Water

Hossain

Sultana

Deb

et al. 2012

Bangla. J. Microbiol.

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To determine the presence of enterohaemorrhagic Escherichia coli (EHEC), 50 water samples of three different lakes in Dhaka, Bangladesh were studied. A total of 90 strains were examined thoroughly to isolate sorbitol non-fermenting (NSF) E. coli. Twelve of 90 isolates (13.3%) from 5 samples were found to be sorbitol nonfermenter, which were further screened for rhamnose, cellobiose and sucrose fermentation. Eleven (12.2%) of 90 isolates also tested negative for cellobiose and sucrose fermentation, while only 3 (3.3%) of them were found to be rhamnose non-fermenting. Biochemical tests confirmed that 91.7% (11 of 12) of NSF strains were indole positive. Eleven NSF E. coli isolates survived at 45.5°C and mostly produced gas by fermentation within 24h. After antimicrobial susceptibility testing, 91.7% (n=11) of these isolates were found resistant to cephalexin, ampicillin and amoxicillin. Ceftazidime and nalidixic acid resistance was also observed in 75% (9 of 12) and 58.3% (7 of 12) isolates, respectively. Plasmid profile analysis revealed that nine (75%) strains harboured large plasmids of >35.8-140 megadaltons (MDa), four (33.3%) of which contained 60-MDa plasmid. Also, the large-plasmid-associated strains shared common behaviour in antimicrobial resistance and eight (88.9%) of these were resistant to four or more antibiotics. Our data suggest a possible association of source water contamination in Bangladesh with multidrug-resistant (MDR) EHEC, which is alarming to public health. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11807 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 33-40

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Plasmid profile of Escherichia coli 0157:H7 from apparently healthy animals

Cited by 29 publications

References 7 publications

Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Environmental impact assessment of Attenda abattoir, Ogbomoso southwestern Nigeria on surface and groundwater quality using geo-electrical imaging and microbiological analysis

Multidrug Resistance in Large-Plasmid-Associated Presumptive Enterohaemorrhagic Escherichia coli Isolated from Contaminated Lake Water

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