Plasmid profiling as an epidemiological marker within Aeromonas salmonicida

Nielson, B.; Olsen, Jane E.; Larsen, J. L.

doi:10.3354/dao015129

Cited by 23 publications

(21 citation statements)

References 7 publications

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“…Methods applied to typical Aeromonas salmonicida such as DNA:DNA reassociation (Belland & Trust 1988), plasmid profiling (Nielsen et al 1993, Sørum et al 2000, ribotyping (Nielsen et al 1994), DNA probes (Gustafson et al 1992), restriction endonuclease analysis (McCormick et al 1990), random amplified polymorphic DNA analysis (RAPD) (Hänninen et al 1995, Miyata et al 1995 and restriction fragment length polymorphism analysis by pulsed field gel-electrophoresis (PFGE) (Livesley et al 1999), all demonstrate the typical isolates to be extremely homogeneous. Similar studies on atypical strains using RAPD analysis (Kwon et al 1997, O'hIci et al 2000, ribotyping (Hänninen & Hirvelä-Koski 1997), PCR-typing (Høie et al 1999), plasmid profiling (Sørum et al 2000) and restriction fragment analysis by PFGE (Umelo & Trust 1998, O'hIci et al 2000, have shown them to be more genetically diverse compared to the typical strains.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic variability and relatedness among atypical Aeromonas salmonicida from marine fishes, using AFLP-fingerprinting

Lund

Jenssen²,

Wesmajervi³

2002

Dis. Aquat. Org.

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Atypical strains of Aeromonas salmonicida are the causal agent of atypical furunculosis or ulcer disease in various fish species, including spotted wolffish Anarhichas minor, which is a promising species in the Norwegian fish-farming industry. Isolates of atypical A. salmonicida comprise a very heterogenous group showing large variety in biochemical, molecular and virulence characteristics. The genetic variability among atypical isolates from wolffish was characterised using amplified fragment length polymorphism analysis: AFLP-fingerprinting. Additional isolates from halibut Hippoglossus hippoglossus, turbot Scophthalmus maximus, cod Gadus morhua and several salmonid fishes were included for assessment of variability and relatedness among a total of 56 atypical isolates of A. salmonicida. They were compared to reference strains of A. salmonicida subspecies and to other Aeromonas species pathogenic in fishes. AFLP-fingerprints subjected to similarity analysis yielded a grouping of the isolates into several clusters, revealing genetic heterogeneity among the isolates. There seems to be a correlation between genetic similarity among isolates and the fish host. The Icelandic isolates, mainly from cod, formed a very homogeneous subcluster, which was closely related to the wolffish isolates. All atypical isolates from spotted and common wolffish grouped together in a large cluster and appear to be very homogeneous, even though they had been isolated over a period of 8 yr at different locations in Norway. On the other hand, most of the isolates from turbot and halibut grouped together into 2 different clusters, while the 9 atypical isolates from salmonids appeared in 4 different clusters. Thus, the atypical isolates of A. salmonicida from halibut, turbot and salmonid fishes seem to be more genetically diverse than those from wolffish and cod.

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Section: Introductionmentioning

confidence: 99%

Assessment of genetic variability and relatedness among atypical Aeromonas salmonicida from marine fishes, using AFLP-fingerprinting

Lund

Jenssen²,

Wesmajervi³

2002

Dis. Aquat. Org.

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“…DNA:DNA reassociation analysis (Belland & Trust 1988), plasmid profiling (Nielsen et al 1993), ribotyping (Nielsen et al 1994), DNA probes (Gustafson et al 1992, Hiney et al 1992. restriction endonuclease analysis (McCormick et al 1990), random amplified polymorphic DNA analysis (Hanninen et al 1995, Miyata et al 1995, and pulsed-field gel electrophoresis (PFGE) (Umelo & Trust 1998) have concentrated on A. salmonicida subsp.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the fish pathogen Aeromonas salmonicida demonstrated by random amplified polymorphic DNA and pulsed-field gel electrophoresis analyses

O'Hici

Olivier²,

Powell

2000

Dis. Aquat. Org.

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The current taxonomy of Aeromonas salmonicida includes 4 subspecies. A . salrnonicida subsp. salmonicida is associated with salrnonid furunculosis, and A. salmon~cida subsp. achromogenes, A. salmonicida subsp. masoucida, and A. salmonicida subsp. smithia are strains that show variation in some biochenlical properties. This classification does not readly encompass isolates from a wide range of fish hosts currently described as atypical A. salmonic~da. This study examined 17 typical strains, 39 atypical strains and 3 type A. sahonicida subspecies strains for genetic similarity using the random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) techniques. On the basis of RAPD-and PFGE-derived profiles, similarity matrices and dendrograms were constructed. The results showed that species A. salmonicida constituted a genetically heterogeneous group of strains, encompassing within an homogeneous or clonal lineage comprised solely of typical strains and the A. salmonicida subsp. salmonicida type strain.

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“…; Byers et al . ), including 25% of 57 examined Danish strains (Nielsen, Olsen & Larsen ). Some authors explain the missing plasmid by stressful culturing conditions above 22–25°C, which was shown to activate an pAsal1 insertion sequence element IS AS11 that leads to the loss of the plasmid (Daher et al .…”

Section: Discussionmentioning

confidence: 99%

Detection and quantification of Aeromonas salmonicida in fish tissue by real‐time PCR

Bartkova

Kokotovic

Skall

et al. 2016

Journal of Fish Diseases

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Furunculosis, a septicaemic infection caused by the bacterium Aeromonas salmonicida subsp. salmonicida, currently causes problems in Danish seawater rainbow trout production. Detection has mainly been achieved by bacterial culture, but more rapid and sensitive methods are needed. A previously developed real-time PCR assay targeting the plasmid encoded aopP gene of A. salmonicida was, in parallel with culturing, used for the examination of five organs of 40 fish from Danish freshwater and seawater farms. Real-time PCR showed overall a higher frequency of positives than culturing (65% of positive fish by real-time PCR compared to 30% by a culture approach). Also, no real-time PCR-negative samples were found positive by culturing. A. salmonicida was detected by real-time PCR, though not by culturing, in freshwater fish showing no signs of furunculosis, indicating possible presence of carrier fish. In seawater fish examined after an outbreak and antibiotics treatment, real-time PCR showed the presence of the bacterium in all examined organs (1-482 genomic units mg ). With a limit of detection of 40 target copies (1-2 genomic units) per reaction, a high reproducibility and an excellent efficiency, the present real-time PCR assay provides a sensitive tool for the detection of A. salmonicida.

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Plasmid profiling as an epidemiological marker within Aeromonas salmonicida

Cited by 23 publications

References 7 publications

Assessment of genetic variability and relatedness among atypical Aeromonas salmonicida from marine fishes, using AFLP-fingerprinting

Assessment of genetic variability and relatedness among atypical Aeromonas salmonicida from marine fishes, using AFLP-fingerprinting

Genetic diversity of the fish pathogen Aeromonas salmonicida demonstrated by random amplified polymorphic DNA and pulsed-field gel electrophoresis analyses

Detection and quantification of Aeromonas salmonicida in fish tissue by real‐time PCR

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