Plasmid profiling of indigenous Bacillus thuringiensis isolated from Tamil Nadu and Kashmir

Reyaz, Arshi; Shahanaj, I.; Gunapriya, L.; D, Nancy; Karthik, C.; Arulselvi, P. Indra

doi:10.1016/j.jopr.2013.03.007

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…The more plasmids exist in an organism, the more specific is the plasmid profile as a marker for a single isolate. Most Bacillus species, regardless of their source, harbour at least one indigenous plasmid (Molina-Aja et al, 2002;Reyaz et al, 2013;Lobova et al, 2015). These bacterial isolates have further corroborated the submission of Gao et al (2011) that Bacillus spp., have evolved several genetic mediated mechanisms to survive in extreme environment.…”

Section: Resultssupporting

confidence: 58%

Plasmid Profiles of Extremophiles Associated with Indigenous Black Soaps and Functional Groups Present

Oyeniran¹,

Oladunmoye²

2015

Microbiology J.

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In the present study, plain indigenous black soap market sample and black soap fortified with brown and white eggshell powder effectively at 20 g/250 mL of molten soap were analyzed microbiologically using standard method after which the bacterial isolates were profiled for presence of plasmid. Structural elucidation of the black soaps was achieved by Gas Chromatography Mass Spectrometry (GCMS) analysis of the methylated fraction. Fungal isolates from plain black soap were Chrysosporium spp. and Aspergillus granulosus while the bacterial isolate: Bacillus vedderi contained plasmid with an estimated molecular weight of 17578 bp. Ramulispora javanicus and Aspergilus flavus while Bacillus vedderi and Bacillus faraginis were fungal isolates were also recorded from black soap fortified with brown and white eggshell powder, respectively. The GCMS analysis revealed fatty acid as the predominant functional group. Plasmid presence in these bacteria may confer extremophily status on them and thus aid their survival in this environment. Antimicrobial activities of the indigenous black in folkloric applications may be predicated on the presence of its fatty acid compounds.

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Section: Resultssupporting

confidence: 58%

Plasmid Profiles of Extremophiles Associated with Indigenous Black Soaps and Functional Groups Present

Oyeniran¹,

Oladunmoye²

2015

Microbiology J.

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“…This may be due to, the isolates have gained the plasmid that carry cry gene from the prevalent strains and appear as positive Bt strain (Table 1). As the ICPs producing gene is located in the plasmid and in the chromosome (Reyaz et al 2013). The plasmid might have been transmitted…”

Section: Discussionmentioning

confidence: 99%

Field Evaluation of Native B. thuringiensis Isolates Against Aphids (Aphis fabae)

Gc¹,

Arjyal²

2020

TU J. Microbiol.

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Objectives: The purpose of this study was to evaluate the aphicidal activity of native Bacillus thuringiensis (Bt) strains. Methods: Soil samples of Provinces 2 and 3 of Nepal were collected randomly for isolation of Bt by acetate selection method. Bt were identified by observing insecticidal crystal proteins (ICPs) by Commassie Brilliant Blue (CBB) staining technique. Aphicidal activity of 12 B. thuringiensis isolates was evaluated by two processes. The preliminary screening was done by spraying the suspension containing the spore and ICPs mixture in Phaseolus species heavily infested with black aphids (Aphis fabae) in fi elds. The second process (selective bioassay) was done by counting the number of aphids (nymphs, instar, winged, wingless) before and after spraying 5ml of suspension containing the spore and ICPs mixture on the leaf or on the beans pods surface infested by Aphids. The mortality percentage of Aphids after treatment was calculated on the 4th day, by counting the live aphids and the result was recorded. Results: Preliminary screening for aphicidal activity revealed that 4 isolates ML5(1), CW1(1), SN2(1) and MP2(1) producing spherical crystal protein, showed 100% mortality against nymphs, instar, winged and wingless Aphids. Isolates were effective in controlling the Aphid (Aphis fabae) within 4 days and the part of the plant that was sprayed becomes free of Aphids. Selective bioassay of native isolate MP3(3) was most effective in killing 95.83% of aphids followed by CW2(1), 85.71%, ML5(1), 77.34%, SN3(1), 72.72%, CW1(1), 70.21%. Conclusion: This study revealed that indigenous Bacillus thuringiensis of Terai region of Nepal are effective in controlling Aphids.

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Cry1 holotype toxins of Bacillus thuringiensis

Lone

Lone²,

Sharma

et al. 2013

Journal of Pharmacy Research

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Plasmid profiling of indigenous Bacillus thuringiensis isolated from Tamil Nadu and Kashmir

Cited by 3 publications

References 17 publications

Plasmid Profiles of Extremophiles Associated with Indigenous Black Soaps and Functional Groups Present

Plasmid Profiles of Extremophiles Associated with Indigenous Black Soaps and Functional Groups Present

Field Evaluation of Native B. thuringiensis Isolates Against Aphids (Aphis fabae)

Cry1 holotype toxins of Bacillus thuringiensis

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