Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter

Klingmüller, Walter

doi:10.1016/0378-1097(91)90506-6

Cited by 19 publications

(4 citation statements)

References 12 publications

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“…Conjugal gene transfer has also been observed, in some cases, in conditions not optimal for bacterial growth in which, in some reports, conjugation seems even more efficient ( Richaume et al . 1989 ; Klingmüller 1991). It was shown that a soil moisture content of 8% was associated with maximum transfer frequency, while the highest number of donors and recipients was found at 20% moisture ( Richaume et al .…”

Section: Introductionmentioning

confidence: 99%

Colonization strategies and conjugal gene transfer of inoculatedPseudomonas syringaeon the leaf surface

Björklöf

Nurmiaho‐Lassila

Klinger

et al. 2000

Journal of Applied Microbiology

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2000. Survival, colonization and activity of Pseudomonas syringae bacteria inoculated onto the leaf surface of the common bean (Phaseolus vulgaris) was studied. Inoculated Ps. syringae cells shortened by half their size in 100% humidity and by an average of one ®fth in 40± 60% humidity. The respiring portion of the population, measured by the formation of 5-cyano-2,3-ditolyl tetrazolium chloride (CTC)-formazan crystals, decreased more in 40±60% humidity than in 100% humidity. In scanning electron micrographs, the bacterial cells on leaf surfaces were seen embedded in a mucoid matrix. Intraspecies conjugation of plasmid RP1 also occurred in 40±60% humidity conditions. The portion of transconjugants temporally rose higher than the same portion in 100% humidity conditions. Therefore, although only a small proportion of the inoculated cells remained active on the leaf surface in 40±60% humidity, a relatively high rate of conjugation was still seen. Gene spreading was thus ef®cient on the leaf surface also when conditions did not allow bacterial population growth.

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Section: Introductionmentioning

confidence: 99%

Colonization strategies and conjugal gene transfer of inoculatedPseudomonas syringaeon the leaf surface

Björklöf

Nurmiaho‐Lassila

Klinger

et al. 2000

Journal of Applied Microbiology

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“…The most important advantage of the procedure, however, was that the resulting DNA, in contrast to that recovered by other procedures, did not inhibit any of the restriction endonucleases tested. Thus it was possible to trace particular DNA sequences of E.agglomerans 19-1-1 in soil samples inoculated with these bacteria as described by and Klingmiiller, (1991). As shown in Figure 1 Tn5 and nif specific sequences were detected in samples analysed 70 days after the introduction of the inoculant bacteria.…”

Section: Molecular Analysis Of Nucleic Acids Recovered From Soilmentioning

confidence: 99%

The Release of Genetically Modified Microorganisms—REGEM 2

Stewart‐Tull¹,

Sussman²

1992

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“…“BacteriaGame”, an educational card game about bacteriology was employed in a range of educational settings. Compared to a traditional lecture, playing the “BacteriaGame” resulted in a significantly higher learning outcome 34 . Indeed, the implementation of active learning methods and gamification into education on science, technology, engineering, and mathematics (STEM) has demonstrated a positive influence on both student achievement and motivation 35, 36 , also particularly benefiting disadvantaged students 37 , but an interactive game that explains the enormous progress of microbiome research for our health is missing.…”

Section: Introductionmentioning

confidence: 97%

“Tiny Biome Tales”: a gamified review about the influence of lifestyle choices on the human microbiome

Schweitzer,

Wlasak,

Wassermann

et al. 2024

Preprint

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In the last two decades, new discoveries from microbiome research have changed our understanding of human health. It became evident that daily habits and lifestyle choices shape the human microbiome and ultimately determine health or disease. Therefore, we developed “Tiny Biome Tales” (https://microbiome.gamelabgraz.at/), a science pedagogy video game designed like a scientific review based exclusively on peer-reviewed articles, to teach about the influence of lifestyle choices on the human microbiome during pregnancy, early and adult life, and related health consequences. Despite the scientific character, it can be played by a broad audience. Here, we also present a scientific assessment, and showed that playing the game significantly contributed to knowledge gain. The innovative style of the “gamified review” represents an ideal platform to disseminate future findings from microbiome research by updating existing and adding new scenes to the game.

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Plasmid transfer in natural soil: a case by case study with nitrogen-fixing Enterobacter

Cited by 19 publications

References 12 publications

Colonization strategies and conjugal gene transfer of inoculatedPseudomonas syringaeon the leaf surface

Colonization strategies and conjugal gene transfer of inoculatedPseudomonas syringaeon the leaf surface

The Release of Genetically Modified Microorganisms—REGEM 2

“Tiny Biome Tales”: a gamified review about the influence of lifestyle choices on the human microbiome

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