Plasmodesmata and Long-Distance Virus Movement

Derrick, P. M.; Nelson, Steven E.

doi:10.1007/978-3-642-60035-7_17

Cited by 11 publications

(2 citation statements)

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“…Madore & Lucas, 1989; Milburn & Kallarackal, 1989). Compared with the many studies on virus cell‐to‐cell movement, however, there have been fewer studies describing virus movement in the vasculature and its accumulation throughout the plant at the tissue level (reviewed in Derrick & Nelson, 1999; Santa Cruz, 1999). An important issue requiring further study is the identification of virus entry and exit sites into and out of the phloem that function during systemic infection.…”

Section: Introductionmentioning

confidence: 99%

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Vascular invasion routes and systemic accumulation patterns of tobacco mosaic virus in Nicotiana benthamiana

Cheng

Carter

et al. 2000

The Plant Journal

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SummaryPlant viruses must enter the host vascular system in order to invade the young growing parts of the plant rapidly. Functional entry sites into the leaf vascular system for rapid systemic infection have not been determined for any plant/virus system. Tobacco mosaic virus (TMV) entry into minor, major and transport veins from non-vascular cells of Nicotiana benthamiana in source tissue and its exit from veins in sink tissue was studied using a modi®ed virus expressing green¯uorescent protein (GFP). Using a surgical procedure that isolated speci®c leaf and stem tissues from complicating vascular tissues, we determined that TMV could enter minor, major or transport veins directly from non-vascular cells to produce a systemic infection. TMV ®rst accumulated in abaxial or external phloem-associated cells in major veins and petioles of the inoculated leaf and stems below the inoculated leaf. It also initially accumulated exclusively in internal or adaxial phloem-associated cells in stems above the inoculated leaf and petioles or major veins of sink leaves. This work shows the functional equivalence of vein classes in source leaves for entry of TMV, and the lack of equivalence of vein classes in sink leaves for exit of TMV. Thus, the specialization of major veins for transport rather than loading of photoassimilates in source tissue does not preclude virus entry. During transport, the virus initially accumulates in speci®c vascularassociated cells, indicating that virus accumulation in this tissue is highly regulated. These ®ndings have important implications for studies on the identi®cation of symplasmic domains and host macromolecule vascular transport.

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Section: Introductionmentioning

confidence: 99%

“…Previous studies indicate that this structural differentiation has functional consequences for photoassimilate transport (reviewed in Turgeon, 1989). However, the role of each phloem type for photoassimilate or virus transport is not fully understood ( Derrick & Nelson, 1999; Turgeon, 1989).…”

Section: Introductionmentioning

confidence: 99%