Plasmodesmata‐located protein overexpression negatively impacts the manifestation of systemic acquired resistance and the long‐distance movement of Defective in Induced Resistance1 in <i><scp>A</scp>rabidopsis</i>

Carella, Philip; Isaacs, Marisa; Cameron, Robin K.

doi:10.1111/plb.12234

Cited by 48 publications

(40 citation statements)

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“…Consistent with previous reports, total protein levels were higher in phloem exudates collected from SARinduced compared with mock-inoculated leaves (Champigny et al, 2013;Carella et al, 2015), which may suggest that the induction of SAR leads to the mass translocation of a number of proteins through the phloem. If this is indeed true, then significant modifications to companion cell plasmodesmatal pore size are likely required to facilitate increased protein loading into the phloem.…”

Section: The Sar-induced Phloem Proteomesupporting

confidence: 91%

“…To obtain protein levels suitable for LC-MS/MS, exudates from more than 90 plants per treatment were collected and concentrated using centrifugal concentrators (3-kD cutoff) followed by lyophilization. Similar to previous reports (Champigny et al, 2013;Carella et al, 2015), phloem exudates collected from SAR-induced leaves contained higher total protein levels than exudates collected from mock-induced leaves (Supplemental Fig S1B). Concentrated phloem exudates from two independent experimental replicates were subjected to quantitative LC-MS/MS (Supplemental Data S1).…”

Section: Quantitative Proteomics Of Phloem Exudates During Sarsupporting

confidence: 89%

“…This suggests that DIR1 is a member of a large proteinaceous complex that travels to distant leaves in the phloem during SAR. Additionally, total protein levels are typically higher in phloem exudates collected from SAR-induced versus mock-inoculated leaves (Champigny et al, 2013;Carella et al, 2015), supporting the notion that numerous proteins are loaded into the phloem during SAR.…”

mentioning

confidence: 63%

“…Interestingly, the results did not preclude additional mechanisms of transport, as SAR signal movement was not strictly limited to the orthostichy (vascular bundle) of the induced leaf, suggesting that SAR signals move cell to cell from one orthostichy to another to better disseminate the signal. This idea was supported recently by the observation that plant lines with reduced cell-to-cell movement through plasmodesmata are defective in SAR and the longdistance movement of DEFECTIVE IN INDUCED RESISTANCE1 (DIR1; Carella et al, 2015). Taken together, these studies demonstrate that long-distance SAR signaling is dependent on the phloem for efficient interorgan communication.…”

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confidence: 79%

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Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

et al. 2016

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ORCID IDs: 0000-0002-5467-7290 (P.C.); 0000-0002-3422-4083 (J.M.-P.).Systemic acquired resistance (SAR) is a plant defense response that provides long-lasting, broad-spectrum pathogen resistance to uninfected systemic leaves following an initial localized infection. In Arabidopsis (Arabidopsis thaliana), local infection with virulent or avirulent strains of Pseudomonas syringae pv tomato generates long-distance SAR signals that travel from locally infected to distant leaves through the phloem to establish SAR. In this study, a proteomics approach was used to identify proteins that accumulate in phloem exudates in response to the induction of SAR. To accomplish this, phloem exudates collected from mock-inoculated or SAR-induced leaves of wild-type Columbia-0 plants were subjected to label-free quantitative liquid chromatography-tandem mass spectrometry proteomics. Comparing mock-and SAR-induced phloem exudate proteomes, 16 proteins were enriched in phloem exudates collected from SAR-induced plants, while 46 proteins were suppressed. SAR-related proteins THIOREDOXIN h3, ACYL-COENZYME A-BINDING PROTEIN6, and PATHOGENESIS-RELATED1 were enriched in phloem exudates of SAR-induced plants, demonstrating the strength of this approach and suggesting a role for these proteins in the phloem during SAR. To identify novel components of SAR, transfer DNA mutants of differentially abundant phloem proteins were assayed for SAR competence. This analysis identified a number of new proteins (m-type thioredoxins, major latex protein-like protein, ULTRAVIOLET-B RESISTANCE8 photoreceptor) that contribute to the SAR response. The Arabidopsis SAR phloem proteome is a valuable resource for understanding SAR long-distance signaling and the dynamic nature of the phloem during plant-pathogen interactions.

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Section: The Sar-induced Phloem Proteomesupporting

confidence: 91%

Section: Quantitative Proteomics Of Phloem Exudates During Sarsupporting

confidence: 89%

mentioning

confidence: 63%

mentioning

confidence: 79%

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Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

et al. 2016

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“…The signal transduction process and its timing are essential components of SAR induction that moves from induced to distant leaves. Recent studies indicated that the movement of SAR signal(s) is connected to plasmodesmata [10].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of TMV Lesion Formation and Timing of Signal Transduction during Induction of Systemic Acquired Resistance (SAR) in Tobacco with a Computer-Assisted Method

Nagy¹,

Kátay²,

Gullner³

et al. 2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

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Nicotiana tabacum L. cv. Xanthi nc plants were inoculated with tobacco mosaic virus (TMV) in order to develop a method for evaluation of lesion size and its distribution characteristics during the induction of systemic acquired resistance (SAR). All necrotic lesions were scored with an image analysis software and subjected to statistical analysis. The diminished lesion size and its right-skewed, non-normal distribution seem to be an important feature of SAR response. The results showed that the degree of induced resistance differs according to the position of the leaf on the plant's shoot. In order to detect the timing of signal transduction from TMV infected leaves to distant ones, the infected leaves were removed from the tobacco plants at different time intervals. When the infected leaves were removed after 4 days, the SAR was always induced on the distant leaves indicating complete signal transduction within 4 days.

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Systemic Signalling in Plant Defence

Vlot

Pabst

Riedlmeier

2017

Encyclopedia of Life Sciences

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Systemic signalling involves a complex network of signal transduction and amplification that leads to the activation of defence genes and establishment of systemic resistance throughout the entire plant. On microbial invasion, pathogen‐associated molecular patterns and effectors are frequently detected and recognised by plant receptors. Localised perception at the infection site rapidly triggers a cascade of early signalling events, including protein phosphorylation and production of reactive oxygen species. Subsequently, secondary signal molecules are synthesised and involved in amplification of defence signalling and the establishment of systemic acquired resistance (SAR). An increasing number of long‐distance signalling intermediates has been identified. Most of these act together and specifically promote systemic defence via the regulation of the local release of a set of systemically mobile signals. In the systemic tissue, the induction of SAR depends on synergistic interactions between systemic and ubiquitous salicylic acid‐associated immune signals. Key Concepts Local resistance to pathogen infection generally results from PAMP (pattern‐associated molecular pattern)‐triggered immunity and microbial effector‐triggered immunity. Microbial infection of local tissues often leads to systemic acquired resistance (SAR) in distal tissues which provides long‐lasting broad‐spectrum resistance to a variety of pathogens. Reactive oxygen species mediate systemic immunity acting as both cell‐to‐cell systemic signals and as local signalling partners upstream of other phloem‐mobile signals. Mobile signals for SAR that are translocated via the vasculature may include methyl salicylate, the C 9 lipid peroxidation product azelaic acid, one or more lipid transfer proteins, the diterpene dihydroabietinal and the nonprotein amino acid pipecolic acid. Many of the SAR mobile signals cooperate with each other and with salicylic acid in one or more parallel and synergistic signalling pathways. As a form of priming, SAR is supported by epigenetic regulation of defence gene expression.

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Plasmodesmata‐located protein overexpression negatively impacts the manifestation of systemic acquired resistance and the long‐distance movement of Defective in Induced Resistance1 in Arabidopsis

Cited by 48 publications

References 41 publications

Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

Comparative Proteomics Analysis of Arabidopsis Phloem Exudates Collected During the Induction of Systemic Acquired Resistance

Evaluation of TMV Lesion Formation and Timing of Signal Transduction during Induction of Systemic Acquired Resistance (SAR) in Tobacco with a Computer-Assisted Method

Systemic Signalling in Plant Defence

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