Plasmodiophora brassicae infection threshold—how many resting spores are required for generating clubroot galls on canola (Brassica napus)

Zahr, Kher; Yang, Yalong; Sarkes, Alian; Dijanovic, Snezana; Fu, Han; Harding, Michael W.; Feindel, David; Feng, Jie

doi:10.1007/s41348-022-00565-z

Cited by 4 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also performed disease severity assessments following inoculation with a higher inoculum concentration (1.0 x 10 6 resting spores/mL water). While a similar trend was observed in terms of DI values, the differences were not significant (Figure S1B) , which led us to work with the lower spore concentration as reported in other studies (Zahr et al, 2022). Collectively, these results indicate that AIL7 plays a role in clubroot resistance in Arabidopsis.…”

Section: Resultssupporting

confidence: 84%

See 1 more Smart Citation

Functions of the AP2/ERF family transcription factor AIL7 in immunity against soilborne clubroot pathogen in Arabidopsis

Jayawardhane,

Somarathna,

Manoli

et al. 2024

Preprint

View full text Add to dashboard Cite

Soilborne pathogens can be highly devastating, and clubroot, caused by Plasmodiophora brassicae, is particularly destructive to cruciferous plants. Although many AP2/ERF family transcription factors (TFs) have crucial physiological functions, very little is known regarding their functions in the context of soilborne diseases. Here we investigated the roles of AINTEGUMENTA-LIKE 7 (AIL7), an AIL sub-family TF in the AP2/ERF family, in plant immunity against clubroot. Unexpectedly, both AIL7 overexpression and mutant Arabidopsis lines exhibited increased tolerance to P. brassicae. Subsequent analysis revealed significant transcriptional alterations in genes linked to pathogen response, along with notable differences in genes associated with salicylic acid (SA) and jasmonic acid (JA) defense pathways, compared to wild-type plants. Interestingly, there was a tendency for up-regulation of SA- and JA-related genes in AIL7 overexpression and mutant lines in the absence, rather than presence, of P. brassicae. Subsequent phytohormone analyses confirmed these results. Taken together, AIL7 has an important role in maintaining constitutive systemic acquired resistance, involving phytohormone mediated defense, and this, rather than an accumulation of SA following P. brassicae challenge, primes the plants for improved clubroot resistance, which would shed light on exploring the functions of other AP2/ERF family TFs in plant immunity against soilborne pathogens.

show abstract

Section: Resultssupporting

confidence: 84%

“…Agronomy, 9(10), 589. https://doi.org/10.3390/agronomy9100589 Gan, C., Yan, C., Pang, W., Cui, L., Fu, P., Yu, X., Qiu, Z., Zhu, M., Piao, Z., & Deng, X. (2022).…”

Section: Total Rna Extraction Cdna Synthesis and Quantitative Pcrmentioning

confidence: 99%

Functions of the AP2/ERF family transcription factor AIL7 in immunity against soilborne clubroot pathogen in Arabidopsis

Jayawardhane,

Somarathna,

Manoli

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The resting spores of P. brassicae were diluted to 1 × 10 5 spores mL −1 ( Zahr et al, 2022 ) with Hoagland’s nutrient solution, and 10 mL was added to each 10 mL centrifuge tube. The concentration of actinomycete sterile filtrate was adjusted, so that it was diluted by 1-, 2-, and 5-fold in the resting spore suspension.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Streptomyces melanosporofaciens strain X216 at controlling clubroot disease on oilseed rape

Ding,

Zhou,

Liang

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Oilseed rape (Brassica napus L.) is highly susceptible to infection from the soilborne pathogen Plasmodiophora brassicae Woronin that causes clubroot disease and deleteriously affects production throughout the world. In this study, biological control resources were explored by isolating 237 strains of bacteria from fields of oilseed rape using the gradient dilution coating method. A strain with strong antagonistic ability was screened using a plate confrontation test and designated X216. It was identified as Streptomyces melanosporofaciens owing to its morphological characteristics and 16S rRNA gene sequence. This study also examined the lethality of strain X216 to the resting spores of P. brassicae, its influence on infection in root hairs, and its ability to control clubroot on oilseed rape. The corrected lethality rate on resting spores after strain X216 had been used for 14 days was 56.59% ± 1.97%, which was significantly higher than the use of 75% of the fungicides chlorothalonil WP and 20% Fluazinam SC. Significantly fewer root hairs were infected after this treatment. A pot test showed that X216 was 62.14% effective at controlling the disease, which was not significantly different from that of the fungicide 100 g L−1 cyazofamid SC diluted 1,000-fold but significantly higher than those of 75% chlorothalonil and 50% carbendazim WP. Strain X216 controlled 43.16% of the incidence of clubroot in the field, which could significantly reduce the disease index of oilseed rape clubroot. Therefore, strain X216 is promising to study for the biological control of oilseed rape clubroot.

show abstract

Bacterial consortium based on Pseudomonas fluorescens, Lysinibacillus xylanilyticus and Bacillus velezensis reduces clubroot disease in broccoli

Moreno-Velandia,

Izquierdo-García,

García-Arias

et al. 2024

Eur J Plant Pathol

View full text Add to dashboard Cite

Clubroot disease caused by Plasmodiophora brassicae is the most devastating disease in Brassicaceae plants. Control of clubroot is limited because of the survival of resting spores of the pathogen in the soil for years. Crop rotation, liming of the soil, fungicides and resistant cultivars have not been totally efficient in reducing the damages of the disease or pathogen spread. Although biocontrol with microorganisms has shown potential against clubroot, the variable results when using individual strains have made implementation difficult. Thus, the aim of this work was to determine whether a consortium of selected beneficial microorganisms is more efficient in controlling clubroot in broccoli than single strains. Single strains of bacteria from the Lysinibacillus, Bacillus and Pseudomonas genera (5 × 107 cells ml−1) and Trichoderma spp. (1 × 106 conidia ml−1) were evaluated in an initial screening. Four strains showing the best performance on plant growth and reduction of clubroot were evaluated as consortia in a further experiment. Treatments were applied in nursery (4 ml plant−1), and to the soil one week before transplant and the day of transplant (60 ml plant−1 each time). Plant shoot and root dry weight and the incidence and severity of clubroot were recorded. Supernatant from liquid cultures of Pseudomonas migulae Pf014 and Lysinibacillus xylanilyticus Br042, and washed cells of Bacillus velezensis Bs006 and B. pumilus Br019 promoted shoot growth of the plants. Single applications of Trichoderma asperellum Th034, Bs006, Br042 and Pseudomonas fluorescens Ps006 showed biocontrol potential against clubroot. However, the consortia built of Bs006, Br042 and Ps006 showed the highest reduction of clubroot incidence (78%) and severity (74%). A synergistic interaction by this consortium was found compared with the efficacy of single strains. To our knowledge, this work reports for the first time a consortium based on three rhizobacteria with high potential to control clubroot disease.

show abstract

Plasmodiophora brassicae infection threshold—how many resting spores are required for generating clubroot galls on canola (Brassica napus)

Cited by 4 publications

References 28 publications

Functions of the AP2/ERF family transcription factor AIL7 in immunity against soilborne clubroot pathogen in Arabidopsis

Functions of the AP2/ERF family transcription factor AIL7 in immunity against soilborne clubroot pathogen in Arabidopsis

Efficacy of Streptomyces melanosporofaciens strain X216 at controlling clubroot disease on oilseed rape

Bacterial consortium based on Pseudomonas fluorescens, Lysinibacillus xylanilyticus and Bacillus velezensis reduces clubroot disease in broccoli

Contact Info

Product

Resources

About