Plasmodium CDP-DAG synthase: An atypical gene with an essential N-terminal extension

Identification of CDP-Archaeol Synthase, a Missing Link of Ether Lipid Biosynthesis in Archaea

“…The apparent K m for DGGGP (measured with constant detergent concentration) was calculated as 0.12 ± 0.02 mM, which only serves as an indication for the affinity toward the lipid substrate due to the presence of detergent in the reaction. The K m for PA of CDS from various organisms has been studied before and in general ranges from 0.28 mM (E. coli) to 0.9 mM (Plasmodium falciparum) (Martin et al, 2000;Shastri et al, 2010), and thus the K m of CarS for DGGGP compares favorable with those studies. In some studies, the K m was measured in constant lipid:detergent ratio, thereby taking surface dilution kinetics (Carman et al, 1995) into account.…”

New Export Pathway in Plasmodium falciparum‐Infected Erythrocytes: Role of the Parasite Group II Chaperonin, PfTRiC

“…Differently, the proteins were detected in the urea soluble fractions when iRBCs were subjected to the same extraction protocols. To confirm and localize these soluble forms, iRBC fractionation was performed as previously described ( Materials and Methods ). PfSBP1, PfE60 and PfJ23 were detected in both the parasitophorous vacuole (PV) soluble fraction and the iRBC cytoplasm soluble fraction from Pf3D7‐infected erythrocytes while membrane‐associated in the parasite (Figure C, SolPar).…”

“…The transgenic Plasmodium falciparum 3D7 /pARL2‐ Pfsbp 1‐ gfp , Pf3D7/pARL2‐P fsbp 1Δ 1–150 ‐ gfp , Pf3D7/pARL2‐P fsbp 1Δ 26–210 ‐ gfp and Pf3D7/pARL2‐P fsbp 1Δ 1–210 ‐ gfp transgenic lines were continuously selected for resistance to 9 nM WR99210 for the maintenance of human DHFR‐based pARL 2 vectors .…”

“…Differential extraction from gelatin‐enriched trophozoites and ghost preparations were performed as described previously using 6 M urea, 100 mM sodium carbonate pH 11, 1% Triton X100 and 1% SDS. The soluble and insoluble fractions were separated by 1 h centrifugation at 100 000 g.…”

Transposon mutagenesis identifies genes essential for Plasmodium falciparum gametocytogenesis

“…It has been hypothesized that the parasite must use some sort of signaling system to sense changes in the outside environment that eventually results in commitment to sexual differentiation (45). P. falciparum CDP-DAG contains an N-terminal extension that localizes the protein to the parasitophorous vacuole (46) and thus, could play a role in the signal transduction system involved in commitment to gametocytogenesis.…”