Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice

Soulard, Valérie; Bosson-Vanga, Henriette; Lorthiois, Audrey; Roucher, Clémentine; Franetich, Jean François; Zanghì, Gigliola; Bordessoulles, Mallaury; Tefit, Maurel; Thellier, Marc; Morosan, Serban; Naour, Gwenola Le; Capron, Frédérique; Suemizu, Hiroshi; Snounou, Georges; Moreno-Sabater, Alicia; Mazier, Dominique

doi:10.1038/ncomms8690

Cited by 108 publications

(105 citation statements)

References 51 publications

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“…HSC-engrafted mice have few circulating human RBC due to mouse macrophage-mediated phagocytosis making infection of P. falciparum limited unless daily RBC injections are administered (64). Complete P. falciparum replication has been possible using human liver chimeric Fah null Rag2 null IL2rg null (FRG) NOD and thymidine kinase (TK) NOG mice engrafted with human hepatocytes and infused with human RBCs (65, 66). Humanized mice are now being used to study P. falciparum genetic traits and phenotypes associated with severity of disease (67) and for testing the efficacy of new therapeutics that block entry of the virus into hepatocytes (68).…”

Section: Infectious Disease In Humanized Micementioning

confidence: 99%

Humanized Mouse Models of Clinical Disease

Walsh

Kenney

Jangalwe

et al. 2017

Annu. Rev. Pathol. Mech. Dis.

453

384

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Immunodeficient mice engrafted with functional human cells and tissues, i.e., “humanized mice”, have become increasingly important as small pre-clinical animal models for the study of human diseases. Since the description of immunodeficient mice bearing mutations in the IL2 receptor common gamma chain (IL2rgnull) in the early 2000’s, investigators have been able to engraft murine recipients with human hematopoietic stem cells that develop into functional human immune systems. These mice can also be engrafted with human tissues such as islets, liver, skin, and most solid and hematologic cancers. Humanized mice are permitting significant progress in studies of human infectious disease, cancer, regenerative medicine, graft versus host disease, allergies, and immunity. Ultimately, use of humanized mice may lead to the implementation of truly “personalized” medicine in the clinic. This review discusses recent progress in the development and use of humanized mice, and highlights their utility for the study of human diseases.

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Section: Infectious Disease In Humanized Micementioning

confidence: 99%

Humanized Mouse Models of Clinical Disease

Walsh

Kenney

Jangalwe

et al. 2017

Annu. Rev. Pathol. Mech. Dis.

453

384

View full text Add to dashboard Cite

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“…parasites, allowing for sporozoite penetration and further development of EEFs [21]. More recently, humanized mice have been successfully used to observe the development of P. ovale liver stages, including hypnozoites [22]. Our experiments show that Huh7 cells, which are derived from human cellular carcinoma cells, also support development of P. ovale spp EEFs.…”

Section: Discussionmentioning

confidence: 58%

Mosquito Transmission and Human Hepatocyte Infections With Plasmodium ovale curtisi and P. ovale wallikeri

Kristan

Thorburn

Hafalla

et al. 2018

Preprint

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Human ovale malaria is caused by the two closely related species Plasmodium ovale curtisi and P. ovale wallikeri. Both species are known to relapse from quiescent hepatic forms months or years after the primary infection occurred. Although some studies have succeeded in establishing mosquito transmission for ovale malaria, none have specifically described transmission and human hepatocyte infection of both sibling species. Here we describe a simplified protocol for successful transmission of both P. ovale curtisi and P. ovale wallikeri to Anopheles coluzzii mosquitoes, and streamlined monitoring of infection using sensitive parasite DNA detection, by loop-activated amplification, in blood-fed mosquitoes. In one experimental infection with P. ovale curtisi and one with P. ovale wallikeri, viable sporozoites were isolated from mosquito salivary glands, and used to successfully infect cultured human hepatocytes. This protocol provides a method for the utilisation of pre-treatment clinical blood samples from ovale malaria patients, collected in EDTA, for mosquito infection studies and generation of the hepatic life cycle stages of P. ovale curtisi and P. ovale wallikeri. We also demonstrate the utility of LAMP as a rapid and sensitive alternative to dissection for estimating the prevalence of infection in Anopheles mosquitoes fed with Plasmodiuminfected blood.

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“…The complete life cycle of Plasmodium falciparum , a protozoan parasite causing tropical malaria, requires both the hepatic and erythrocytic phases of proliferation. TK‐NOG mice engrafted with human hepatocytes and infused daily with human RBCs supported the full life cycle of Plasmodium falciparum , opening a way to investigate the efficacy of anti‐malaria therapy in a small animal model (Soulard et al, ).…”

Section: Humanized Liver Micementioning

confidence: 99%

“…Heuts et al (2013) Plasmodium farciparum Both RBC and liver phases of replication TK-NOG engrafted with hepatocytes and infused with RBCs repeatedlySoulard et al (2015) Leishmania majorInfection to macrophages, T-cell immune responses NSG engrafted with HSCs Wege et al (2012) NOG, NOD/Shi-scid Il2rg −/− ; BRG, Balb/c Rag2 −/− Il2rg −/− ; NSG, NOD/LtSz-scid Il2rg −/− ; NSG-A2, NSG with HLA-A2 transgene; PAM, aminobisphosphonate pamidronate; HTLV-1, human T-cell leukemia virus; KSHV, Kaposi's sarcoma-associated herpesvirus; HCMV, human cytomegalovirus; HSV-2, herpes simplex virus type 2; HDV, hepatitis D virus; HEV, hepatitis E virus; JCV, JC virus; LPD, lymphoproliferative disorder; GPCs, glial progenitor cells; BRG-AFC8, BRG with a transgene encoding a fusion protein of the FK506 binding protein and caspase 8. FUJIWARA | 2895 difficult to directly translate the results of preclinical studies in laboratory animals to clinical practice.…”

mentioning

confidence: 99%

“…A study with HCV-infected humanized NOD Fah −/− Rag2 −/− IL-2rg −/− mice indicated that treatment with broadly neutralizing antibodies can abrogate established HCV infection in vivo(de Jong et al, 2014).The complete life cycle of Plasmodium falciparum, a protozoan parasite causing tropical malaria, requires both the hepatic and erythrocytic phases of proliferation. TK-NOG mice engrafted with human hepatocytes and infused daily with human RBCs supported the full life cycle of Plasmodium falciparum, opening a way to investigate the efficacy of anti-malaria therapy in a small animal model(Soulard et al, 2015).…”

mentioning

confidence: 99%

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Humanized mice: A brief overview on their diverse applications in biomedical research

Fujiwara

2017

Journal Cellular Physiology

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Model animals naturally differ from humans in various respects and results from the former are not directly translatable to the latter. One approach to address this issue is humanized mice that are defined as mice engrafted with functional human cells or tissues. In humanized mice, we can investigate the development and function of human cells or tissues (including their products encoded by human genes) in the in vivo context of a small animal. As such, humanized mouse models have played important roles that cannot be substituted by other animal models in various areas of biomedical research. Although there are obvious limitations in humanized mice and we may need some caution in interpreting the results obtained from them, it is reasonably expected that they will be utilized in increasingly diverse areas of biomedical research, as the technology for preparing humanized mice are rapidly improved. In this review, I will describe the methodology for generating humanized mice and overview their recent applications in various disciplines including immunology, infectious diseases, drug metabolism, and neuroscience.

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Plasmodium falciparum full life cycle and Plasmodium ovale liver stages in humanized mice

Cited by 108 publications

References 51 publications

Humanized Mouse Models of Clinical Disease

Humanized Mouse Models of Clinical Disease

Mosquito Transmission and Human Hepatocyte Infections With Plasmodium ovale curtisi and P. ovale wallikeri

Humanized mice: A brief overview on their diverse applications in biomedical research

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