Plasmodium gallinaceum: Sporozoite Invasion of Aedes aegypti Salivary Glands Is Inhibited by Anti-gland Antibodies and by Lectins

Barreau, Catherine; Touray, Musa; Pimenta, Paulo; Miller, Liane; Vernick, Kenneth D.

doi:10.1006/expr.1995.1124

Cited by 61 publications

(61 citation statements)

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“…Other studies have indicated that the salivary gland components are highly glycosylated (2) and that specific lectins can inhibit sporozoite invasion of the salivary glands (3). In this study, we did not attempt to determine whether the 29-kDa or 100-kDa A. gambiae salivary gland proteins were part of the basal lamina or the secretory cells.…”

Section: Discussionmentioning

confidence: 91%

“…Studies investigating the possibility of blocking sporozoite invasion of mosquito salivary glands used a fixed number of sporozoites injected intrathoracically along with anti-salivary gland antibodies (3). Although assays performed in this manner may serve to evaluate more accurately the role of salivary gland proteins in sporozoite invasion, we feel that our assay better emulates the natural course of malaria transmission, but with it comes the inherent variability of any biological system.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it has been shown that lectins that bind salivary gland-associated carbohydrates block Plasmodium gallinaceum (avian malaria parasite) sporozoite invasion of Aedes aegypti salivary glands. Concurrently, polyclonal serum against A. aegypti salivary glands inhibited P. gallinaceum sporozoite invasion as compared with preimmune serum and saline controls (3).…”

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confidence: 92%

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Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites

Brennan

Kent

Dhar

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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Anopheles gambiae is the primary vector of human malaria in sub-Saharan Africa. Invasion of Anopheles salivary glands by Plasmodium sporozoites is a necessary step in the transmission of malaria and is likely to be mediated by specific receptor-ligand interactions. We are interested in identifying putative an A. gambiae salivary gland receptor or receptors for sporozoite invasion as a possible target for blocking malaria transmission. By using monoclonal antibodies against female-specific A. gambiae salivary gland proteins, two molecules, one of 29 kDa and one of 100 kDa, were identified and characterized with respect to the age and blood-feeding process of mosquitoes. In an in vivo bioassay, the monoclonal antibody against the 100-kDa protein inhibited Plasmodium yoelii sporozoite invasion of salivary glands >75%. These results show that A. gambiae salivary gland proteins are accessible to monoclonal antibodies that inhibit sporozoite invasion of the salivary glands and suggest alternate targets for blocking the transmission of malaria by this most competent of malaria vectors.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 92%

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Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites

Brennan

Kent

Dhar

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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“…The mechanisms by which sporozoites localize to and invade salivary glands are not known, although their target cell specificity suggests that these are receptor-mediated events. Recent work demonstrating that antibodies to molecules on the salivary gland surface can inhibit sporozoite invasion supports this hypothesis [4].…”

Section: Introductionmentioning

confidence: 95%

Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum

Sidjanski

Vanderberg

Sinnis

1997

Molecular and Biochemical Parasitology

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In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but evidence suggests that it is a receptor-mediated process. Here we show that the major surface protein of sporozoites, the circumsporozoite protein (CS), binds preferentially to salivary glands when compared to other organs exposed to the circulating hemolymph. In addition, we show that a peptide encompassing region I, a highly conserved sequence found in all rodent and primate Plasmodium CS proteins, inhibits binding of CS to mosquito salivary glands.

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“…Sporozoites are formed in a complex process, termed sporogony, that represents the longest developmental phase of the Plasmodium life cycle [3]. Although sporozoites are likely passively transported throughout the hemocoel, there is evidence that they specifically recognize the salivary glands through a receptor-mediated process [4][5][6][7]. Sporozoites breach the basal lamina and acinar gland cells and accumulate in the salivary duct [8].…”

Section: Introductionmentioning

confidence: 99%

Transgenic Plasmodium berghei sporozoites expressing β-galactosidase for quantification of sporozoite transmission

Engelmann¹,

Sinnis²,

Matuschewski³

2006

Molecular and Biochemical Parasitology

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Malaria transmission occurs during a blood-meal of an infected Anopheles mosquito. Visualization and quantification of sporozoites along the journey from the mosquito midgut, where they develop, to the vertebrate liver, their final target organ, is important for understanding many aspects of sporozoite biology. Here we describe the generation of Plasmodium berghei parasites that express the reporter gene lacZ as a stable transgene, under the control of the sporozoite-specific CSP promoter. Transgenic sporozoites expressing β-galactosidase can be simply visualized and quantified in an enzymatic assay. In addition, these sporozoites can be used to quantify sporozoites deposited in subcutaneous tissue during natural infection.

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Plasmodium gallinaceum: Sporozoite Invasion of Aedes aegypti Salivary Glands Is Inhibited by Anti-gland Antibodies and by Lectins

Cited by 61 publications

References 0 publications

Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites

Anopheles gambiae salivary gland proteins as putative targets for blocking transmission of malaria parasites

Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum

Transgenic Plasmodium berghei sporozoites expressing β-galactosidase for quantification of sporozoite transmission

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