Plasmolysis of<i>Escherichia coli</i>B/r with Sucrose

Scheie, Paul

doi:10.1128/jb.98.2.335-340.1969

Cited by 38 publications

(31 citation statements)

References 16 publications

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“…The two can even be simultaneous (Fig. 8) indeed instantaneous, confirming previous results (Schleie & Dalen, 1968;Schleie, 1969. It is very rapidly repaired, provided the energy metabolism of the cell is optimal.…”

Section: Study Of Plasmolysissupporting

confidence: 87%

“…s~ NaC1) in the medium, then the cells frequently have a distorted appearance: they collapse into irregular flat, elongated discs, which, according to their orientation with respect to the axis of observation, appear either similar or unaltered, or as widened cells or distorted, relatively thin rods (Fig. 6), confirming previous reports (Schleie & Dalen, 1968;Schleie, 1969). In contrast to the plasmolytic bays, produced by weaker shocks, these results are difficult to achieve reproducibly.…”

Section: Study Of Plasmolysissupporting

confidence: 81%

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Unstained and in vivo fluorescently stained bacterial nucleoids and plasmolysis observed by a new specimen preparation method for high‐power light microscopy of metabolically active cells

Kellenberger¹,

Kamp²

1994

Journal of Microscopy

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Summary Microscope slides were coated with a layer of gelatin, the thickness of the gelatin increasing linearly along the long axis. The bacterial suspension is applied to the dried gelatin and covered by a coverslip. The medium is absorbed by the gelatin and thus the cells applied against the coverslip. By this method, cultures of concentrations below 108 cells/ml provide statistically relevant numbers for observation without prior concentration steps. It is easier to apply than the existing methods for the observation of bacterial nucleoids by phase contrast imaging. Because the cells are maintained in growing conditions the method is useful for the vital fluorescence DAPI‐staining of various bacterial species and for observations of plasmolysis and its reversal at different physiological conditions and extracellular osmolalities. The previously generally assumed view that the plasmolytic changes of the cell morphology are immediate upon the hyperosmotic shock and are rapidly repaired when the cell is able to metabolize actively was confirmed; this is in contrast to some recent claims.

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Section: Study Of Plasmolysissupporting

confidence: 87%

Section: Study Of Plasmolysissupporting

confidence: 81%

Unstained and in vivo fluorescently stained bacterial nucleoids and plasmolysis observed by a new specimen preparation method for high‐power light microscopy of metabolically active cells

Kellenberger¹,

Kamp²

1994

Journal of Microscopy

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“…But deplasrnolysis obviously takes place in the presence of nutrients and ions as previously observed in N . meningitidis ( 6 ) as well as in other bacteria (12).…”

Section: Discussionmentioning

confidence: 99%

INFLUENCE OF OSMOTIC PRESSURE ON TRANSFORMABLE AND NON TRANSFORMABLE VARIANTS OF NEISSERIA MENINGITIDIS

Jyssum

1975

Acta Pathologica Microbiologica Scandinavica Section B Microbio

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The Neisseria meningitidis Strain M1 could grow in media made hypertonic with 0.7 M sucrose, but the growth rate was much reduced. The reduction was more pronounced in the competent (cp+) variant than in the incompetent (cp‐) one. After exposure to increased osmotic pressure, growth was resumed after a pronounced lag which was regularly longer in the cp+ variant. Cellular lysis took place during the lag. The lag could be shortened by the addition of MgCl2. N. meningitidis cells lysed slowly when suspended in various isotonic solutes, but rapid lysis was activated in exponential phase cells by exposure to hypotonic as well as to hypertonic conditions. This activation was more pronounced in the cp‐ variant than in the cp+ variant. In stationary phase cells there was very little activation of autolysis. The rate of autolysis was inhibited by MgCl2, and CaCl2 in low concentration. Osmotic fragility developed during autolysis in the presence of high concentrations of acetate or sucrose but not in the presence of high concentrations of NaCl or phosphate. MgCl2, increased the osmotic stabilization caused by acetate or sucrose, whereas CaCl2, in low concentration abolished the stabilizing effect of these solutes.

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“…In a further analysis of the consequences of pnd gene expression we have found a drastic effect on the response of cells to hypertonic medium. Ordinarily, E. coli cells are plasmolyzed in such a medium: internal water is lost, the cytoplasm shrinks, and the cytoplasmic membrane is caused to retract from the cell wall (3,8,32). But when we exposed the cells to a hypertonic sucrose solution after the addition of rifampicin to induce the pnd protein, plasmolysis was largely abolished.…”

Section: Induction Of Plasmolysis In Cells Carrying the Pnd Genementioning

confidence: 99%

Nucleotide Sequence of the pnd Gene in Plasmid R483 and Role of the pnd Gene Product in Plasmolysis

Ono

Akimoto

Ohnishi

1987

Microbiology and Immunology

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The pnd gene of R plasmid R483, like the srnB gene of the F plasmid, increases the degradation of stable RNA in Escherichia coli. The nucleotide sequence of the pnd locus was determined and compared with that of the srnB locus. The genes have open reading frames that are 54% homologous, and both have an upstream inverted repeat sequence.The pnd gene expression seems to decrease the osmotic barrier of the cytoplasmic membrane, since no plasmolytic vacuoles were formed in the cells carrying the gene when the cells were exposed to hypertonic sucrose solution.This result suggests that RNase I in the periplasm passes through the altered membrane to degrade stable RNA in the cytoplasm.

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Plasmolysis ofEscherichia coliB/r with Sucrose

Cited by 38 publications

References 16 publications

Unstained and in vivo fluorescently stained bacterial nucleoids and plasmolysis observed by a new specimen preparation method for high‐power light microscopy of metabolically active cells

Unstained and in vivo fluorescently stained bacterial nucleoids and plasmolysis observed by a new specimen preparation method for high‐power light microscopy of metabolically active cells

INFLUENCE OF OSMOTIC PRESSURE ON TRANSFORMABLE AND NON TRANSFORMABLE VARIANTS OF NEISSERIA MENINGITIDIS

Nucleotide Sequence of the pnd Gene in Plasmid R483 and Role of the pnd Gene Product in Plasmolysis

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