“…When the metallic nanoparticles are in close proximity to each other, the limited horizontal and vertical space can cause strong oscillations between the surrounding plasmonic, which is called plasmon coupling. − Plasmons transform a substantial part of the oscillation energy into heat through plasmon coupling effect, which can be detected by PA imaging. ,, Plasmonic coupling can also lead to the electric field in the interparticle gap to be amplified by several orders of magnitude, which far exceeds the field enhancement for a single plasmonic nanoparticle. ,− Generally, in the plasmon resonance system, the plasmon coupling effect is deeply affected by the behavior (separation or aggregation) between metallic nanoparticles, that is, the separation or aggregation of the metallic nanoparticles may cause the reduce or enhance of plasmon coupling effect, respectively (Figure a) . In terms of the absorption spectra, the separation of the metallic nanoparticles can cause a blue-shift of the wavelength, whereas the aggregation (or nucleation) of them may cause a red-shift of the wavelength. ,, And the magnitude of the shift wavelength mainly relies on the particle geometry (size and shape), structure (solid and hollow), spacing, and surroundings of the emitting particle.…”