Plasmonic labeling of subcellular compartments in cancer cells: multiplexing with fine-tuned gold and silver nanoshells

Sobral-Filho, Regivaldo G.; Brito-Silva, Antônio M.; Isabelle, Martin; Jirásek, A; Lum, Julian J.; Brolo, Alexandre G.

doi:10.1039/c6sc04127b

Cited by 28 publications

(23 citation statements)

References 40 publications

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“…Radiosensitizers, such as gold nanoparticles (GNP), can be used to enhance radiation effect in tumors based on the contrast between the electronic properties of gold and soft tissues . The synthetic versatility of GNP permits their fabrication in different sizes, shapes and morphologies. − Surface modification allows the conjugation of GNP with cytotoxic drugs, extending the application of these particles to drug delivery. − …”

Section: Introductionmentioning

confidence: 99%

Collagen Type I–Gelatin Methacryloyl Composites: Mimicking the Tumor Microenvironment

Valente

Thind

Akbari

et al. 2019

ACS Biomater. Sci. Eng.

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Therapeutic drugs can penetrate tissues by diffusion and advection. In a healthy tissue, the interstitial fluid is composed of an influx of nutrients and oxygen from blood vessels. In the case of cancerous tissue, the interstitial fluid is poorly drained because of the lack of lymphatic vasculature, resulting in an increase in interstitial pressure. Furthermore, cancer cells invade healthy tissue by pressing and pushing the surrounding environment, creating an increase in pressure inside the tumor area. This results in a large differential pressure between the tumor and the healthy tissue, leading to an increase in extracellular matrix (ECM) stiffness. Because of high interstitial pressure in addition to matrix stiffening, penetration and distribution of systemic therapies are limited to diffusion, decreasing the efficacy of cancer treatment. This work reports on the development of a microfluidic system that mimics in vitro healthy and cancerous microenvironments using collagen I and gelatin methacryloyl (GelMA) composite hydrogels. The microfluidic device developed here contains a simplistic design with a central chamber and two lateral channels. In the central chamber, hydrogel composites were used to mimic the ECM, whereas lateral channels simulated capillary vessels. The transport of fluorescein sodium salt and fluorescently labeled gold nanoparticles from capillary-mimicking channels through the ECM-mimicking hydrogel was explored by tracking fluorescence. By tuning the hydrogel composition and concentration, the impact of the tumor microenvironment properties on the transport of those species was evaluated. In addition, breast cancer MCF-7 cells were embedded in the hydrogel composites, displaying the formation of 3D clusters with high viability and, consequently, the development of an in vitro tumor model.

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Section: Introductionmentioning

confidence: 99%

Collagen Type I–Gelatin Methacryloyl Composites: Mimicking the Tumor Microenvironment

Valente

Thind

Akbari

et al. 2019

ACS Biomater. Sci. Eng.

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“…Recently, the label-free localized surface plasmon resonance (LSPR) detection technique has been developed for in situ biological detection and cell imaging. − The LSPR signal comes from the coherent oscillation of the conduction electrons in the conduction band of plasmonic nanoparticles. − The scattering light of an individual plasmonic nanoparticle can be observed at the single particle level under a dark-field microscope, providing much improved spatiotemporal resolution than the traditional averaged measurement. − Additionally, LSPR signal mainly depends on the nanoparticle morphology, size, composition, as well as the surrounding dielectric environment. − Therefore, efforts toward the development of plasmonic nanoparticles with different shapes and architectures such as nanoflowers, nanorods, nanocubes, and core–satellites nanostructures have been made to enhance the scattering spectral shift, which are more sensitive to the change of the interface microenvironment on nanoparticle surface. − For example, Wang and co-workers designed a smart plasmonic nanobiosensor based on individual Au@Ag core–shell nanocube modified with tetrahedron-structured DNA for detecting microRNA 21 at the single-molecule level . Yeung and co-workers introduced a Au–Ag core–shell nanorods as a probe for highly sensitive sulfide mapping in live cells based on the Ag 2 S formation .…”

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confidence: 99%

Strategy for In Situ Imaging of Cellular Alkaline Phosphatase Activity Using Gold Nanoflower Probe and Localized Surface Plasmon Resonance Technique

et al. 2018

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In this work, a simple and ultrasensitive localized surface plasmon resonance (LSPR) method that use Au nanoflowers (AuNFs) as a probe was designed for in situ monitoring of alkaline phosphatase (ALP) activity. The AuNFs were fabricated by hydrogen tetrechloroaurate-induced oxidative disruption of polydopamine-coated Au nanoparticles (AuNPs), and subsequently, growth of Au nanopetals on AuNPs occurred. The as-prepared AuNFs showed a much higher LSPR capability and stronger scattering color change than AuNPs. The strategy for in situ cellular ALP activity detection relied on the deposition of Ag on the AuNFs surface, which changed the morphology of AuNFs and led to a tremendous LSPR response and scattering color change. The deposition of Ag shell on AuNFs was related to ALP activity, where ALP catalyzed the hydrolysis of L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate to form Lascorbic acid (AA), and then AA reduced Ag + to Ag and deposited onto AuNFs. With this concept, the ALP activity could be monitored with a detection limit of 0.03 μU L −1 . Meanwhile, the ALP activity of single HepG2 cells and HEK 293 cells was tracked with a proposed approach, which indicated the trace expression level of ALP in HEK 293T cell and overexpressed level of ALP in HepG2 cells. After treatment with drugs, the cellular ALP activity of HepG2 cells was decreased with the treating time and dose increasing. Therefore, the proposed strategy could be used for tracking the cellular ALP activity, which paved a new avenue for cell studies and held great potential for discovering novel ALP-based drugs applications.

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“…Different size and intensity of bright spots could be observed when the nanoparticles bounded to the cell membrane and were internalized into the cellular organelles. Furthermore, antibody‐coated gold shells and nuclear localization signal (NLS)‐coated silver shell targeted the plasma membrane and nuclear membrane, respectively, were synthesized to label different subcellular compartments in MCF‐7 breast cancer cells [59]. Owing to the color‐tuned nanoshells under white light illumination, multiplex analysis at the single cell‐single particle level can be easily achieved in clinical conditions.…”

Section: Dark‐field Light‐scattering Microscopymentioning

confidence: 99%

Recent Advances in Plasmonic Nanostructures Applied for Label‐free Single‐cell Analysis

Liao

Tan

et al. 2021

Electroanalysis

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Cellular heterogeneity presents a major challenge in understanding the relationship between cells of particular genotype and response in disease. In order to characterize the cell‐to‐cell differences during the biochemical processes, single‐cell analysis is necessary. Profiting from the unique localized surface plasmon resonance (LSPR) and Mie scattering, plasmonic nanostructures have revealed stable and adjustable scattering signals, avoiding photobleaching, blinking and autofluorescence phenomenon. These characterizations are propitious to the dynamic trace and biological image of single living cells. In this review, we discuss the recent advances in plasmonic nanostructures applied for label‐free detection and monitoring of target cells at single‐cell level by using three different techniques, surface‐enhanced Raman scattering (SERS), surface‐enhanced Infrared absorption spectroscopy (SEIRAS), and dark‐field microscopy. Various avenues to design plasmonic probes combining spectra and imaging for single‐cell analysis are demonstrated as well. We hope this review can highlight the superiority of plasmonic nanostructures in single cellular analysis, and further motivate the development of label‐free cell analysis technique to elucidate cellular diversity and heterogeneity.

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Plasmonic labeling of subcellular compartments in cancer cells: multiplexing with fine-tuned gold and silver nanoshells

Abstract: Multiplexing at the single cell–single particle level was achieved with fine-tuned nanoshells featuring narrow LSPR bands.

Cited by 28 publications

References 40 publications

Collagen Type I–Gelatin Methacryloyl Composites: Mimicking the Tumor Microenvironment

Collagen Type I–Gelatin Methacryloyl Composites: Mimicking the Tumor Microenvironment

Strategy for In Situ Imaging of Cellular Alkaline Phosphatase Activity Using Gold Nanoflower Probe and Localized Surface Plasmon Resonance Technique

Recent Advances in Plasmonic Nanostructures Applied for Label‐free Single‐cell Analysis

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