Plasmonic rK28 ELISA improves the diagnosis of canine<i>Leishmania</i>infection

Maciel, Marilene Oliveira dos Santos; Soares, Matheus Fujimura; Costa, Sidnei Ferro; Bragato, Jaqueline Poleto; Rebech, Gabriela Torres; Freitas, Jéssica Henrique de; Alves, Graziella Borges; Oliveira, Talita Carolina Bragança de; Bresciani, Kátia Denise Saraiva; Lima, Valéria Marçal Felix de

doi:10.1111/pim.12684

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…rK39) [18] used in different geographical regions [20, 26] highlight the need for diagnostic tests designed that contain as base specific antigens from each geographical region. More specific tests will contribute considerably to the rapid and effective global diagnosis of canine and human VL, as well as decrease the frequency of false-negative reports (~10.5 %) [84] and the degree of cross-reactivity with other diseases (e.g. malaria, intestinal schistosomiasis, and tuberculosis) observed [26, 85].…”

Section: Discussionmentioning

confidence: 99%

Revisiting the heterogeneous global genomic population structure of Leishmania infantum

et al. 2021

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Leishmania infantum is the main causative agent responsible for visceral leishmaniasis (VL), a disease with global distribution. The genomic structure and genetic variation of this species have been widely studied in different parts of the world. However, in some countries, this information is still yet unknown, as is the genomic behaviour of the main antigens used in VL diagnosis (rK39 and rK28), which have demonstrated variable sensitivity and specificity in a manner dependent on the geographic region analysed. The objective of this study was to explore the genomic architecture and diversity of four Colombian L. infantum isolates obtained in this study and to compare these results with the genetic analysis of 183 L. infantum isolates from across the world (obtained from public databases), as well as to analyse the whole rK39 and rK28 antigen sequences in our dataset. The results showed that, at the global level, L. infantum has high genetic homogeneity and extensive aneuploidy. Furthermore, we demonstrated that there are distinct populations of L. infantum circulating in various countries throughout the globe and that populations of distant countries have close genomic relationships. Additionally, this study demonstrated the high genetic variability of the rK28 antigen worldwide. In conclusion, our study allowed us to (i) expand our knowledge of the genomic structure of global L. infantum; (ii) describe the intra-specific genomic variability of this species; and (iii) understand the genomic characteristics of the main antigens used in the diagnosis of VL. Additionally, this is the first study to report whole-genome sequences of Colombian L. infantum isolates.

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Section: Discussionmentioning

confidence: 99%

Revisiting the heterogeneous global genomic population structure of Leishmania infantum

et al. 2021

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“…A great advantage in this technique is its practicality and ease of detection since it does not require reading equipment to interpret the results, as also reported in the research by Maciel et al (2020), who used indirect plasmonic ELISA for the detection of Leishmania spp. infection, which presented greater specificity than the official recommended method by the Ministry of Health in Brazil [ 31 ]. Although PCV2 causes a persistent infection in animals, its detection in blood samples is hindered by its short viremia.…”

Section: Resultsmentioning

confidence: 99%

Development of a Gold Nanoparticle-Based ELISA for Detection of PCV2

Basso,

Cruz,

Vieira

et al. 2024

Pathogens

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In this new methodology, plasmonic ELISA (pELISA) was used to detect Circovirus porcine2 (PCV2) in serum samples without the need for plate reading equipment. This process occurs by adapting the conventional ELISA test with gold nanoparticles (AuNPs) to promote a color change on the plate and quickly identify this difference with the naked eye, generating a dark purple-gray hue when the samples are positive and red when the samples are negative. The technique demonstrated high efficiency in detecting samples with a viral load ≥ 5 log10 copies/mL. Plasmonic ELISA offers user-friendly, cost-effective, and reliable characteristics, making it a valuable tool for PCV2 diagnosis and potentially adaptable for other pathogen detection applications.

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Determination of cardiac disease biomarker by plasmonic sandwich ELISA

Wei

Tang

et al. 2021

Biotech and App Biochem

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Acute myocardial infarction (AMI) is the heart attack happening when the blood flow is terminated to the heart muscles. C-reactive protein (CRP) level is raising significantly in AMI patients after the onset of symptom; also, temporal variations of CRP in plasma of AMI patient have also been found. Quantifying the concentration of CRP helps to identify the condition associated with AMI. Plasmonic enzyme-linked immunosorbent assay (ELISA) was utilized here to identify CRP by the sandwich of aptamer and antibody. Bare-eye CRP detection was achieved by plasmonic ELISA through the aggregation (blue color) of gold nanoparticle in the presence of CRP, whereas in the absence of CRP, it retains its red color (dispersion). Depending on the catalase presence on the ELISA surface, hydrogen peroxide (H 2 O 2 ) controls gold growth and differentiates with color changes. To achieve the lowest detection limit of CRP, H 2 O 2 (200 μM), gold seed (0.2 μM), and streptavidin-catalase (1:500) were found optimal. The detection limit was reached at 0.25 μg/mL, whereas it was 0.5 μg/mL in the CRP-spiked serum. This method of detection system is easier to detect the levels of CRP and helps diagnosing AMI.

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Plasmonic rK28 ELISA improves the diagnosis of canineLeishmaniainfection

Cited by 5 publications

References 30 publications

Revisiting the heterogeneous global genomic population structure of Leishmania infantum

Revisiting the heterogeneous global genomic population structure of Leishmania infantum

Development of a Gold Nanoparticle-Based ELISA for Detection of PCV2

Determination of cardiac disease biomarker by plasmonic sandwich ELISA

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