“…Temperature of medium was recorded by Thermal Camera (Irisys, UK). At irradiation, the temperature of cell suspensions with or without GNPs was lower than 30 o C. The studies of laser interaction with GNPs was attracted significant interest,20,21 moreover, absence of heating of surrounding medium also was proved theoretically by Avetisyan et al22 for 800-nm pulsed (50 ps -50 ns): the calculated temperature on the surface can be up to 100°C, while 10 nm away from the surface in water it hardly reaches 50°C.Preparations of cells were observed by inverted microscope Leica 3000 (Leica Microsystems, Germany) in the fluorescence mode (filter I3, excitation 450-490 nm) at the Simbioz Center, IBPPM RAS, Saratov. Each experiment was triplicated.…”