Plasticity of Mesenchymal Stem Cells from Mouse Bone Marrow in the Presence of Conditioned Medium of the Facial Nerve and Fibroblast Growth Factor-2

Abstract

A number of evidences show the influence of the growth of injured nerve fibers in peripheral nervous system as well as potential implant stem cells (SCs). The SCs implementation in the clinical field is promising and the understanding of proliferation and differentiation is essential. This study aimed to evaluate the plasticity of mesenchymal SCs from bone marrow of mice in the presence of culture medium conditioned with facial nerve explants and fibroblast growth factor-2 (FGF-2). The growth and morphology were assessed for over 72 hours. Quantitative phenotypic analysis was taken from the immunocytochemistry for glial fibrillary acidic protein (GFAP), protein OX-42 (OX-42), protein associated with microtubule MAP-2 (MAP-2), protein β-tubulin III (β-tubulin III), neuronal nuclear protein (NeuN), and neurofilament 200 (NF-200). Cells cultured with conditioned medium alone or combined with FGF-2 showed morphological features apparently similar at certain times to neurons and glia and a significant proliferative activity in groups 2 and 4. Cells cultivated only with conditioned medium acquired a glial phenotype. Cells cultured with FGF-2 and conditioned medium expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. This study improves our understanding of the plasticity of mesenchymal cells and allows the search for better techniques with SCs.

Treatment of Temporal Bone Fractures

“…The animal studies investigated neural and adipose-derived stem cell induction of facial nerve regeneration and appear to demonstrate the feasibility of cell-based Schwann cell generation in vitro, particularly when coupled with material bridge conduits. [38][39][40][41][42][43][44][45] The human case series so far reported that use of bone marrow-derived mesenchymal cells was uncontrolled, plagued by multiple confounding effects and that any positive effects were almost certainly the result of concomitant facial nerve decompression rather than mesenchymal cell transplantation; thus no benefit from the bone marrow derived MSCs can be ascribed. 46 The timing of facial nerve decompression is, as with everything related to the facial nerve, controversial.…”

Adult human neural cells in culture following traumatic brain injury

“…The human nerve cell culture technique utilized in the present study was adapted from Lucena et al 29 , as follows: under laminar flow, low Knockout Dulbecco's modified Eagle's cell culture medium (DMEM) (GIBCO Thermo Fisher Scientific, Waltham, MA, USA) was supplemented with 10% fetal bovine serum (Sigma Company, St Louis, MO, USA) and 10 µg/mL ceftriaxone (Cultilab, Campinas, SP, Brazil) (D-10 medium). The nervous tissue was then incubated in 4 mL of this medium and placed in sterile 15 mL Falcon tubes for cell suspension.…”

Culture Technique of Human Nervous Cells Collected from Traumatized Brains

“…After collection, this material was immediately stored in Leibovitz-15 preservative medium (L-15: GIBCO Invitrogen Corporation) and shipped for treatment and plating on the addition of D-10 culture medium, being maintained in CO2 greenhouse. The technique of human nerve cell culture was adapted from the work of Lucena et al (Lucena et al, 2014) -performed with rodents -with some modifications and described in detail in item 2.7 below.…”