Plasticity of Proinflammatory Macrophages Depends on Their Polarization Stage during Human MSC Immunomodulation—An In Vitro Study Using THP-1 and Human Primary Macrophages

Ißleib, Constantin; Kurz, Susanne; Scholl, Samuel; Amberg, Bettina; Spohn, Juliane

doi:10.3390/immuno1040036

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…Depending on the factors that induce polarization of macrophages, phagocytosis in some cases in M1 exceeds that observed in M2 [15], in others -phagocytosis is higher in M2 [16,17]. In vitro studies show that the co-cultivation of MSCs and macrophages leads to the expression by macrophages of genes characteristic of the M2 phenotype and an increase of phagocytosis [18,19]. This result was also observed in our in vivo study.…”

Section: Effects Of Mscs Intraperitoneal Injection On Abdominal Cavit...supporting

confidence: 87%

Study of the interaction between macrophages and human umbilical cord MSCs in vivo on the model of perotonitis in mice

et al. 2022

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Mesenchymal stem cells (MSCs) have unique properties that ensure the regeneration of damaged tissues, which allows using them in the cell therapy of various diseases. The realization of these properties is mainly related to the interaction of MSCs with macrophages. Aim. To study the interaction of the human umbilical cord MSCs with macrophages in vivo using a model of sterile inflammation of the peritoneal cavity in mice. Methods. Cytological methods for assessing acute inflammation of the peritoneal cavity of mice, isolation and cultivation of the human umbilical cord MSCs, study of the expression of the MSC surface markers by flow cytometry, determination of the phagocytic activity of mononuclear cells of peritoneal cavity, isolation of mononuclear RNA, RT-PCR, methods of statistical analysis. Results. The dynamics of the development of the MSCs' therapeutic effect after their transplantation into the peritoneal cavity of mice was studied; a method for assessing the therapeutic activity of various MSC preparations was developed; the fast polarization of macrophages to the M2 state after the injection of MSCs was shown, by studying the phagocytic activity of macrophages and expression of the IL-10 gene. Conclusions. The most effective method to increase the therapeutic potential of MSCs is preconditioning with low doses of H 2 O 2 . K e y w o r d s: MSCs, macrophages, peritonitis.

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Section: Effects Of Mscs Intraperitoneal Injection On Abdominal Cavit...supporting

confidence: 87%

Study of the interaction between macrophages and human umbilical cord MSCs in vivo on the model of perotonitis in mice

et al. 2022

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In vitro phenotypic effects of Lipoxin A4 on M1 and M2 polarized macrophages derived from THP-1

et al. 2022

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MSCs’ conditioned media cytokine and growth factor profiles and their impact on macrophage polarization

et al. 2023

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Background There is a growing body of evidence that multipotent mesenchymal stromal cells’ (MSCs’) remarkable therapeutic potential is attributed not only to their differentiation and regenerative capacity, but also to the paracrine effect, underlying their immunomodulatory properties. MSCs’ secretome (i.e., cytokines, growth factors, and extracellular vesicles) is therefore increasingly discussed in the context of their ability to modulate inflammatory response and promote regeneration. There is evidence that 2D or 3D culturing conditions have an impact on the cells’ secretome, and here we aimed to compare the secretion of cytokines and growth factors in human MSCs from different sources cultured in 2D and 3D conditions and assess their effect on human macrophages polarization in vitro. Methods MSCs were derived from human adipose tissue, bone marrow, gingiva, placenta, and umbilical cord, cultured as monolayers or as cell spheroids. Their cytokine profiles were analyzed, and data standardization was carried out using a z-score. Human peripheral blood mononuclear cells-derived macrophages were then treated with umbilical cord-derived MSCs’ conditioned media and their effect on macrophages polarization was assessed. Results Our findings suggest that umbilical cord-derived MSCs’ conditioned media demonstrated the highest cytokine and growth factor levels and despite mostly pro-inflammatory cytokine profile were able to promote anti-inflammatory macrophage polarization. Conclusions Umbilical cord-derived MSCs’ conditioned media hold great potential for therapeutic use, demonstrating significant anti-inflammatory effect on human macrophages.

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Plasticity of Proinflammatory Macrophages Depends on Their Polarization Stage during Human MSC Immunomodulation—An In Vitro Study Using THP-1 and Human Primary Macrophages

Cited by 4 publications

References 32 publications

Study of the interaction between macrophages and human umbilical cord MSCs in vivo on the model of perotonitis in mice

Study of the interaction between macrophages and human umbilical cord MSCs in vivo on the model of perotonitis in mice

In vitro phenotypic effects of Lipoxin A4 on M1 and M2 polarized macrophages derived from THP-1

MSCs’ conditioned media cytokine and growth factor profiles and their impact on macrophage polarization

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