Plastidic Δ6 Fatty-Acid Desaturases with Distinctive Substrate Specificity Regulate the Pool of C18-PUFAs in the Ancestral Picoalga Ostreococcus tauri

Abstract

Osteococcus tauri plastidic lipid C18-PUFA remodelling involves two plastid-located cytochrome-b5 fused Δ6-desaturases with distinct preferences for both head-group and acyl-chain.

Temperature Acclimation of the Picoalga Ostreococcus tauri Triggers Early Fatty-Acid Variations and Involves a Plastidial ω3-Desaturase

“…Nicotiana benthamiana plants were cultivated in a greenhouse under controlled conditions (16–8 h photoperiod, 25°C). Agrobacterium tumefaciens strain GV3101 was grown in Luria Broth medium at 30°C as previously described ( Degraeve-Guilbault et al, 2020 ).…”

“…Ostreococcus tauri transformation was achieved using the pOtOXLuc vector and electroporation and transgenics were pre-screened accordingly to their luminescent level as previously described ( Degraeve-Guilbault et al, 2020 ). Control lines are transgenics of empty vectors.…”

“…Nicotiana benthamiana were transformed by agroinfiltration of leaves from five-week old plants as previously described ( Degraeve-Guilbault et al, 2020 ). Co-infiltration of RNA-silencing inhibitor P19 (equal volume of a bacterial suspension harboring pBin61-P19), was used in all experiments ( Shah et al, 2013 ).…”

“…This procedure allowed for increasing the resolution of minor FA detection without any column saturation with major FA. Lipid extraction was performed as previously described ( Degraeve-Guilbault et al, 2020 ). Briefly, the material was extracted using glass beads in chloroform:methanol (2:1 v/v), pelleted and extracted again until no pigment could be extracted.…”

“…( Supplementary Figure 8). According to the PredAlgo prediction software the cTP score of Otpω3-Des was the highest among all desaturases (4.53/5) (Tardif et al, 2012;Degraeve-Guilbault et al, 2020).…”