Platelet activation of platelet concentrates derived from buffy coat and apheresis methods

Ali, Soleimany Ferizhandy

doi:10.1016/j.transci.2010.12.002

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…3), in agreement with the results of Soleimany et al (2011), where they concluded that the extent of in vitro activation is dependent on the preparation method [33]. Vasallo et al [34] showed that platelets obtained from PWB express higher levels of P-selectin than platelets from PAP.…”

Section: Discussionsupporting

confidence: 85%

Evaluation of store lesion in platelet obtained by apheresis compared to platelet derived from whole blood and its impact on the in vitro functionality

Quintero

Nunez

Mellado

et al. 2015

Transfusion and Apheresis Science

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Section: Discussionsupporting

confidence: 85%

Evaluation of store lesion in platelet obtained by apheresis compared to platelet derived from whole blood and its impact on the in vitro functionality

Quintero

Nunez

Mellado

et al. 2015

Transfusion and Apheresis Science

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“…As a consequence, when PLTs are removed from the blood and are put in an artificial environment, a substantial degree of PLT activation can be demonstrated . Production and storage of PLTs for transfusion represent no exception to this notion . The extent to which these manipulations compromise PLT function is not fully known, but it is nevertheless important to characterize situations in which aberrant PLT activation may occur during PLT production.…”

Section: Discussionmentioning

confidence: 99%

Random aggregates in newly produced platelet units are associated with platelet activation and release of the immunomodulatory factors sCD40L and RANTES

et al. 2013

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“…PCs can be produced by a variety of manufacturing processes which affect more or less the viability and reactivity of PLTs. Several publications report on this subject [3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Transmission Electron Microscopy of Platelets FROM Apheresis and Buffy-Coat-Derived Platelet Concentrates

Neumüller¹,

Ellinger²

2015

The Transmission Electron Microscope - Theory and Applications

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Platelet concentrates are produced in order to treat bleeding disorders. They can be provided by apheresis machines or by pooling of buffy coats from four blood donations. During their manufacturing and storage, morphological alterations of platelets occur which can be demonstrated by transmission electron microscopy. Alterations range from slight and reversible changes, such as formation of small cell protrusions and swelling of the surface-connected open canalicular system, to severe structural changes, where platelets undergo transitions from discoid to ameboid shapes as a consequence of platelet activation. These alterations end in delivery of the contents of platelet granules as well as platelet involution caused by apoptosis and necrosis processes denoted as the platelet release reaction. Hereby, the involvement of the network of the open canalicular system, helping to deliver the contents of platelet granules into the surrounding milieu via pores, is distinctly shown by electron tomography. As a consequence of platelet activation, a delivery of differently sized microparticles takes place which is thought to play an important role in the adverse reactions in some recipients of platelet concentrates. In this article, the formation and delivery of platelet microparticles is illustrated by electron tomography. Above all, the ultrastructural features of platelets and megakaryocytes are discussed in the context of the molecular characteristics of the plasma membrane and organelles including the different granules and the expression of receptors engaged in signaling during platelet activation. Starting from the knowledge of the ultrastructure of resting and activated platelets, a score classification is presented, allowing the evaluation of different activation stages in a reproducible manner. Examples of evaluations of platelet concentrates using electron microscopy are briefly reviewed. In the last part,

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Platelet activation of platelet concentrates derived from buffy coat and apheresis methods

Cited by 10 publications

References 15 publications

Evaluation of store lesion in platelet obtained by apheresis compared to platelet derived from whole blood and its impact on the in vitro functionality

Evaluation of store lesion in platelet obtained by apheresis compared to platelet derived from whole blood and its impact on the in vitro functionality

Random aggregates in newly produced platelet units are associated with platelet activation and release of the immunomodulatory factors sCD40L and RANTES

Transmission Electron Microscopy of Platelets FROM Apheresis and Buffy-Coat-Derived Platelet Concentrates

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