Platelet adhesion and human umbilical vein endothelial cell cytocompatibility of biodegradable segmented polyurethanes prepared with 4,4′-methylene bis(cyclohexyl isocyanate), poly(caprolactone) diol and butanediol or dithioerythritol as chain extenders

Abstract

Biodegradable segmented polyurethanes were prepared with poly(caprolactone) diol as a soft segment, 4,4'-methylene bis(cyclohexyl isocyanate) (HMDI) and either butanediol or dithioerythritol as chain extenders. Platelet adhesion was similar in all segmented polyurethanes studied and not different from Tecoflex® although an early stage of activation was observed on biodegradable segmented polyurethane prepared with dithioerythritol. Relative viability was higher than 80% on human umbilical vein endothelial cells in contact with biodegradable segmented polyurethane extracts after 1, 2 and 7 days. Furthermore, both biodegradable segmented polyurethane materials supported human umbilical vein endothelial cell adhesion, spreading, and viability similar to Tecoflex® medical-grade polyurethane. These biodegradable segmented polyurethanes represent promising materials for cardiovascular applications.

Fabrication and characterization of medical grade polyurethane composite catheters for near-infrared imaging

“…Pressed films (Plain TPU and TPU Composite) were sterilized by washing in 1× PBS for 24 hours under constant agitation (Argos Rotoflex), followed by a 30 minute soak in 100% ethanol and two 1 hour rinses with sterile PBS. Biocompatibility studies were based off work previously completed by Chan-Chan et al (18). Briefly, 12-well cell bind plates were seeded with HUVEC cells (passage 4-10, cultured in complete endothelial growth medium EGM Bulletkit, Lonza) at a density of 100 cells/cm 2 for 12 hours (37°C, 5% CO 2 ) to allow for adhesion.…”

Rational design of biodegradable thermoplastic polyurethanes for tissue repair

“…The HDI-based PU showed higher tensile strength and breaking strain and larger plastic deformation than the BDI-based PU because of its less-ordered hard segments. The cyclohexane rings in cycloaliphatic diisocyanates, such as IPDI and HMDI, limit the flexibility of the polymer chains, and results in stiffer PUs compared with those based on aliphatic linear diisocyanates [ 35 , 36 ]. Furthermore, the introduction of LDI can result in a high hydrolytic degradation rate because it may affect the aggregation and hydrophilicity of hard segments due to the side group containing a hydrolyzable ester bond [ 32 , [37] , [38] , [39] ].…”

On arginine‐based polyurethane‐blends specific to vascular prostheses

“…Although platelet adhesion experiments were not conducted on blends, pristine polymers showed low values. [19][20][21] The mechanical properties obtained in accordance with D-438ASTM standards provided the intrinsic mechanical properties of the blends as shown in Figure 6. An attempt was made to predict modulus (E Theo ) variation with composition by applying the rule of mixtures:…”