Platelet Aggregation and Antibacterial Effects of an L‐Amino Acid Oxidase Purified from Bothrops alternatus Snake Venom.

Stábeli, Rodrigo G.; Marcussi, Silvana; Carlos, Guilherme B.; Pietro, Rosemeire Cristina Linhari Rodrigues; Selistre-de-Araújo, Heloísa Sobreiro; Giglio, José Roberto; Oliveira, Eduardo B.; Soares, Andreimar Martins

doi:10.1002/chin.200436208

Cited by 95 publications

(32 citation statements)

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“…A protein with LAAO activity was previously isolated and partially characterised from the venom of P. australis [28]. However, no protein or gene sequence was described for this snake, or from any other Australian elapid; primary research focus on snake venom LAAOs has been their Gene identifi cation in O. scutellatus characterisation from vipers [17,18]. A full-length product was identifi ed in at least three Australian snakes, O. scutellatus, P. australis and N. scutatus, with a signifi cant degree of identity in deduced protein sequences among the three species.…”

Section: Discussionmentioning

confidence: 99%

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Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species

Pierre

Woods

Earl

et al. 2005

Cell. Mol. Life Sci.

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Australian terrestrial elapid snakes contain amongst the most potently toxic venoms known. However, despite the well-documented clinical effects of snake bite, little research has focussed on individual venom components at the molecular level. To further characterise the components of Australian elapid venoms, a complementary (cDNA) microarray was produced from the venom gland of the coastal taipan (Oxyuranus scutellatus) and subsequently screened for venom gland-specific transcripts. A number of putative toxin genes were identified, including neurotoxins, phospholipases, a pseudechetoxin-like gene, a venom natriuretic peptide and a nerve growth factor together with other genes involved in cellular maintenance. Venom gland-specific components also included a calglandulin-like protein implicated in the secretion of toxins from the gland into the venom. These toxin transcripts were subsequently identified in seven other related snake species, producing a detailed comparative analysis at the cDNA and protein levels. This study represents the most detailed description to date of the cloning and characterisation of different genes associated with envenomation from Australian snakes.

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Section: Discussionmentioning

confidence: 99%

“…A putative toxin, L-amino acid oxidase (LAAO) was previously identifi ed in a number of vipers [17,18]. To investigate the presence of this gene in the venom glands of Australian elapids, PCR was performed using primers designed from an alignment of a number of these snakes.…”

Section: Microarray Hybridisation Analysis and Screeningmentioning

confidence: 99%

Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species

Pierre

Woods

Earl

et al. 2005

Cell. Mol. Life Sci.

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“…L-amino acid oxidase from viper venom has been shown to have antimicrobial and platelet aggregation inhibition activities, while 5′ nucleotidase from the cobra has been demonstrated to have anti-coagulant activity. 35,36 Identification of Toxin Gene Transcripts. The results described above demonstrate the presence of at least ten protein families in the venom of D. vestigiata as determined by 2D gel electrophoresis and mass spectrometric analysis.…”

Section: Identification Of Venom Proteins By 2d Page and Massmentioning

confidence: 99%

Diversity of Toxic Components from the Venom of the Evolutionarily Distinct Black Whip Snake, Demansia vestigiata

et al. 2007

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Included among the more than 300 species of elapid snakes worldwide is the Australian genus Demansia, or whip snakes. Despite evidence to suggest adverse clinical outcomes from envenomation by these snakes, together with confusion on their true phylogenetic relationship to other Australian elapids, not a single toxin sequence has previously been reported from the venom of a Demansia species. We describe here a combined proteomic and transcriptomic approach characterizing the venom from the black whip snake, Demansia vestigiata. A total of 13 distinct toxin families were identified, including homologues of all of the major toxic components previously reported from the venom of other Australian elapids, such as factor X-like prothrombin activators, neurotoxins, phospholipases, cysteine rich secretory proteins, textilinin-like molecules, nerve growth factors, l-amino acid oxidases, vespryns, 5' nucleotidases, metalloproteinases, and C-type lectins as well as a novel dipeptidyl peptidase family. Phylogenetic analysis of these sequences revealed an early evolutionary split of the black whip snake from all other characterized Australian snakes, with a low degree of sequence identity between D. vestigiata and the other snakes, across all toxin families. The results of this study have important implications not only for the further characterization of venom from whip snakes, but also for our understanding of the evolutionary relationship of Australian snake species.

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“…Re-oxidation of FAD by molecular O 2 results, in turn, in the production of keto acids, ammonia and hydrogen peroxide (H 2 O 2 ) (Moustafa et al, 2006;Tan and Fung, 2009). The generated H 2 O 2 can induce (St abeli et al, 2004) and inhibit platelet aggregation (Sakurai et al, 2003;Naumann et al, 2011), induce endothelial injury (Du and Clemetson, 2002) and are involved in the apoptosis of various cell cells due to DNA damage Marcussi et al, 2013;Lee et al, 2014). Bothrops marajoensis is found in the plains of Maraj o Island in the State of Par a, Brazil and possibly along the coastal lowlands of the Amazon Delta, in Brazil (Cavalcante et al, 2011).…”

Section: Introductionmentioning

confidence: 97%

l-amino acid oxidase from Bothrops marajoensis causes nephrotoxicity in isolated perfused kidney and cytotoxicity in MDCK renal cells

Dantas

Jorge

et al. 2015

Toxicon

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Renal alterations caused by Bothrops venom and its compounds are studied to understand these effects and provide the best treatment. Previously, we studied the renal effect of the whole venom of Bothrops marajoensis and its phospholipase A2 (PLA2), but these effects could not to be attributed to PLA2. To continue the study, we report in this short communication the effects of l-amino acid oxidase from B. marajoensis venom (LAAOBm) on renal function parameter alterations observed in the same model of isolated perfused kidney, as well as the cytotoxic effect on renal cells. LAAOBm caused a decrease in PP, RVR, UF, GFR, %TNa(+) and %TCl(-), very similar to the effects of whole venom using the same model. We also demonstrated its cytotoxicity in MDCK cells with IC50 of 2.5 μg/mL and late apoptotic involvement demonstrated by flow cytometry assays. In conclusion, we suggested that LAAOBm is a nephrotoxic compound of B. marajoensis venom.

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Platelet Aggregation and Antibacterial Effects of an L‐Amino Acid Oxidase Purified from Bothrops alternatus Snake Venom.

Cited by 95 publications

References 2 publications

Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species

Identification and analysis of venom gland-specific genes from the coastal taipan (Oxyuranus scutellatus) and related species

Diversity of Toxic Components from the Venom of the Evolutionarily Distinct Black Whip Snake, Demansia vestigiata

l-amino acid oxidase from Bothrops marajoensis causes nephrotoxicity in isolated perfused kidney and cytotoxicity in MDCK renal cells

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