Platelet and plasma coagulation compatibility of heparinized and sulphated surfaces

Larsson, Rolf; Jc, Eriksson; Lagergren, H; Olsson, Per

doi:10.1016/0049-3848(79)90061-6

Cited by 49 publications

(18 citation statements)

References 10 publications

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“…A recent report by Larsson et al [8] does not support this thesis. These work ers demonstrated that surfaces which ap peared to be platelet compatible in in vitro tests revealed positive thrombogenicity in other in vitro and in vivo tests.…”

Section: Discussioncontrasting

confidence: 40%

In vitro Thrombogenicity Test of Materials Used in Arterial Prostheses

Wolf

Grimley²

1983

Pathophysiol Haemos Thromb

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Coagulation rates in fresh, normal, venous blood was investigated when a given volume of blood was allowed to contact various materials of equivalent surface area, at 22 °C, without disturbance. The coagulation rate was determined indirectly by measuring residual thrombin-clottable fibrinogen in cell-free supernatants after arresting coagulation at fixed time intervals with a Ca⁺⁺-chelating type of anticoagulant and comparing the thrombin-clottable fibrinogen content of the test samples with control samples of blood anticoagulated immediately after venipuncture. In glass containers, after initial formation of the clot, progressive fibrin formation, measured by the rate of disappearance of thrombinclottable fibrinogen, was found to take place as a gradual and linear progressive process not complete at 30 min after venipuncture with blood stored undisturbed at 22 °C. Various graft materials in tubular form were prepared to ensure equivalent blood surface contact. These were investigated for their influence on fibrinogen to fibrin conversion of fresh venous blood in a 15-min period at 22 °C. Fibrin formation was greatest in microvel pre-treated with clotting blood and decreased in the following order: microvel, woven Dacron, knitted dacron and Gore-tex. Gore-tex showed by far the least thrombogenicity of all the tested graft materials. We discuss our reasons for making our measurements under non-flow conditions and the reason for measuring fibrinogen to fibrin conversion rather than measuring platelet deposition on the various surfaces.

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Section: Discussioncontrasting

confidence: 40%

In vitro Thrombogenicity Test of Materials Used in Arterial Prostheses

Wolf

Grimley²

1983

Pathophysiol Haemos Thromb

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“…The control surface in our study was sulfated plastic surface, 46 creating negatively charged sulfate groups lacking specificity in growth factor binding. This finding of reduced proliferation on BSA-heparin surface is consistent with a study by Letourneur et al, who demonstrated that immobilized heparin on polysaccharide gel matrices inhibits the growth of EC in vitro, 47 as has previously been shown for immobilized heparin on other matrices.…”

Section: Figmentioning

confidence: 99%

Anchoring of Vascular Endothelial Growth Factor to Surface-Immobilized Heparin on Pancreatic Islets: Implications for Stimulating Islet Angiogenesis

Cabric

Sánchez

Johansson

et al. 2010

Tissue Engineering Part A

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“…The coupling of heparin [6] to PSt-PEO-NH, [5] was performed in a DMF-H,O mixture, first by activating the heparin carboxylic groups with EDC1' at pH 5.1-5.2 and subsequently reacting the activated carboxylic group with the amino groups of the PSt-PEO-NH, [5] at pH 7.5. The yield of coupled heparin was 25 to 29% w/w depending on molecular weight of diblock copolymers.…”

Section: Discussionmentioning

confidence: 99%

“…oxide) Diblock Copolymer [6] A schematic representation of the synthesis of PSt-PEO-NH, diblock copolymers [6] is shown in Scheme 2. PSt-N=C=O 'an determined by VPO in toluene at 37°C.…”

Section: Synthesis Of Amino-semitelechelic Polystyrene-poly(ethy1enementioning

confidence: 99%

“…Copolymer [7] PSt-PEO-Hep [7] was synthesized by a coupling reaction of PSt-PEO-NH, [6] with heparin [6] In the coupling reaction of PSt-PEO-NH, [5] and Hep [6], heparin carboxylic groups are modified and consequently the heparin activity may change. As pointed out by Danishefsky and co-workers20 and Sederel and co-workers,21 modification of heparin carboxylic groups leads to a decreased anticoagulant activity.…”

Section: Synthesis Of Polystyrene-poly(ethy1ene Oxide)-heparin Blockmentioning

confidence: 99%

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Synthesis and characterization of polystyrene–poly(ethylene oxide)–heparin block copolymers

Vulić

Okano

Kim

et al. 1988

J. Polym. Sci. A Polym. Chem.

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SynopsisA procedure for the preparation of new block copolymers composed of a hydrophobic block of polystyrene, a hydrophilic spacer-block of poly(ethy1ene oxide) and a bioactive block of heparin was investigated. Polystyrene with one amino group per chain was synthesized by free radical oligomerhtion of styrene in dimethylformamide, using 2-aminoethanethiol as a chain transfer agent. This amino group was used in the coupling reaction with amino-telechelic poly(ethy1ene oxide) to produce an AB type diblock copolymer with one amino group per polystyrene (PSt)-poly(ethy1ene oxide) (PEO) chain. The amino-semitelechelic oligestyrene was converted into the isocyanatrsemitelechelic oligo-styrene using toluene 2,4diisocyanate and subsequent coupling with H,N-PEO-NH, afforded AB type block copolymers with terminal amino groups.The coupling of PSt-PEO-NH, with heparin was performed in a DMF-H,O mixture, first by activating the heparin carboxylic groups with EDC at pH 5.1-52 and subaequently reading the activated carboxylic groups with the amino groups of the PSt-PEO-NH2 at pH 7.5. Depending on the molecular weights of the diblock copolymer used 25-29% w/w heparin was incorporated. Thew polymers will be further evaluated for their blood-compatibility.

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Platelet and plasma coagulation compatibility of heparinized and sulphated surfaces

Cited by 49 publications

References 10 publications

In vitro Thrombogenicity Test of Materials Used in Arterial Prostheses

In vitro Thrombogenicity Test of Materials Used in Arterial Prostheses

Anchoring of Vascular Endothelial Growth Factor to Surface-Immobilized Heparin on Pancreatic Islets: Implications for Stimulating Islet Angiogenesis

Synthesis and characterization of polystyrene–poly(ethylene oxide)–heparin block copolymers

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