Platelet anti-aggregant property of some Moroccan medicinal plants

Mekhfi, Hassane; Haouari, Mohammed El; Legssyer, Abdelkhaleq; Bnouham, Mohammed; Aziz, Mohammed; Atmani, Fouad; Remmal, Adnane; Ziyyat, Abderrahim

doi:10.1016/j.jep.2004.06.005

Cited by 101 publications

(68 citation statements)

References 24 publications

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“…The ex vivo anti-platelet aggregation activity of the triterpene was determined on thrombin-induced platelet aggregation (Mekhfi et al, 2004). The platelets (200 μl) from the different groups were separately pre-incubated in a 96-well micro plate for 5 min at 37°C before addition of thrombin (20 µl, 5 U/ml).…”

Section: Ex Vivo Anti-platelet Aggregationmentioning

confidence: 99%

Anticoagulant and anti-inflammatory activity of a triterpene from Protorhus longifolia stem bark

Mosa¹,

Ndwandwe²,

Cele³

et al. 2015

J. Med. Plants Res.

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This work evaluated the anticoagulant and anti-inflammatory activity of a lanosteryl triterpene (3β-hydroxylanosta-9,24-dien-21-oic acid) isolated from Protorhus longifolia stem bark. Tail bleeding time assay was used to investigate the ex vivo anticoagulant activity. The effect of the triterpene on the thermally induced aggregation of malate dehydrogenase (MDH) and citrate synthase (CS) was studied. The anti-inflammatory activity of the triterpene was investigated using the cotton pellet-induced granuloma model in rats. Granuloma formation was measured following 7 days of oral administration of the experimental rats (two groups) with the triterpene at 50 and 250 mg/kg body weight (b.w). The compound (50 mg/kg) significantly (p < 0.05) increased bleeding time in rats by up to 7 min as compared to 2.5 min observed in the normal control group. It also improved the activity of Hsp70 on MDH and CS aggregation suppression. The reduction of the granuloma formation by up to 40.3% was observed. It is apparent that the triterpene has potential to inhibit the aggregation of proteins.

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Section: Ex Vivo Anti-platelet Aggregationmentioning

confidence: 99%

Anticoagulant and anti-inflammatory activity of a triterpene from Protorhus longifolia stem bark

Mosa¹,

Ndwandwe²,

Cele³

et al. 2015

J. Med. Plants Res.

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“…It is reported that the leaves of the plant are used as a diuretic, urinary antiseptic, antidiarrheal, anti-inflammatory, antioxidant, astringent and depurative (Pabuçcuoglu et al, 2003;Mariotto et al, 2008;Oliveira et al, 2009). Previous studies have shown that the aqueous extract of A. unedo exhibited vasorelaxant, antidiabetic and antiaggregant actions Mekhfi et al, 2004;Bnouham et al, 2007). Studies regarding the chemical composition of leaves and fruits indicate the presence of phenolic acids, flavonoids, tannins, anthocyanins and vitamins which may be responsible for the pharmacological properties of the plant (Miguel et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Anti-platelet aggregation effects of extracts from Arbutus unedo leaves

Haouari

Mekhfi

2017

Plant Sci. Today

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It is well known that platelet hyperactivity is a risk factor for cardiovascular diseases such as atherosclerosis, stroke and myocardial infarction. This study aimed to examine the effects of extracts enriched in flavonoids obtained from Arbutus unedo leaves on platelet aggregation. Rat platelets were prepared and incubated in vitro with different doses of the tested extracts, and aggregation was trigged by physiological agonists. Platelet treatment with increasing concentrations (0.1 -1 mg/ml) of diethyl ether extract (genins = free flavonoids) or ethyl acetate extract (hetrosidic flavonoids) inhibited platelet aggregation evoked by thrombin in a concentration-dependant manner. The IC50 values were 0.22 ± 0.03 and 0.36 ± 0.05 mg/ml for genins and heterosidic flavonoids respectively. Treatment with Arbutus unedo extracts also significantly reduced the initial rate of platelet aggregation. At 1 mg/ml, the rate inhibition was 97.8 ± 0.74 and 90.8 ± 1.55 % for genins and heterosidic flavonoids respectively. In addition, flavonoids significantly inhibited platelet aggregation induced by ADP, collagen or epinephrine. We conclude that Arbutus unedo extracts show antiaggregant effects due mainly to flavonoids. These results may partly explain the traditional use of Arbutus unedo leaves for the treatment of cardiovascular disorders such as hypertension.

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“…Over activity of the coagulation cascade (hypercoagulation) increases the risk of thromboses formation (Mekhfi et al, 2004). This can easily lead to thromboembolisms which block blood flow and lead to ischemia with subsequent damage to the afflicted organs (Bruno et al, 2001).…”

Section: Introductionmentioning

confidence: 99%