Platelet concentrate in bovine reproduction: effects on in vitro embryo production and after intrauterine administration in repeat breeder cows

Consiglio, A. Lange; Cazzaniga, Nadia; Garlappi, R.; Spelta, Chiara; Pollera, C.; Perrini, C.; Crémonesi, F.

doi:10.1186/s12958-015-0064-6

Cited by 31 publications

(57 citation statements)

References 44 publications

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“…Considering the variability of the responses in superovulation and that none of the treatments are able to influence the recruitment process of small ovarian follicle turnover, the aim of this study was to stimulate the growth of small or preantral follicles by administrating platelet rich plasma (PRP) inside the ovary before the superovulation protocol. This hemocomponent is rich in growth factors and cytokines [16] known for their regenerative properties in human [17] and veterinary medicine [18,19]. Referring to human medicine, Sills et al [20] treated four women with proven infertility for 60 ± 25 months with 5 mL of autologous PRP for each ovary under ultrasound guidance via the transvaginal route.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Embryo Recovery in Holstein Cows Treated by Intra-Ovarian Platelet Rich Plasma before Superovulation

Crémonesi

Bonfanti

Idda

et al. 2020

Veterinary Sciences

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The current research was designed to evaluate if intra-ovarian administration of autologous platelet rich plasma (PRP) before superovulation could increase the number of follicles responsive to gonadotropin treatment in order to improve embryo recovery in donor cows. Eight Holstein-Friesian cows of proven fertility were employed. After estrous synchronization, at the 18th day of diestrous, the right ovary of each cow was left untreated and served as control while the left ovary was inoculated with 5 mL of PRP. Cows were left to spontaneously return to estrous, and nine days later, a standard superovulation was initiated for every cow. Seven days after artificial insemination (AI), putative embryos were collected by flushing the right and left uterine horns separately. All statistics were calculated by ANOVA. The mean number of follicles, evaluated by transrectal ultrasound scanning, did not statistically differ before PRP treatment between right (control) and left (treated) ovaries (9.18 ± 1.35 and 7.32 ± 1.67, p = 0.28, respectively) as well as at 48 h after PRP injection (7.67 ± 2.52 and 8.00 ± 2.00, p = 0.73, respectively). A statistical (p = 0.023) difference was found in the average number of follicles at the last gonadotropin injection between control and treated ovaries (11.33 ± 2.89 and 20.00 ± 9.17, respectively). The statistically different (p = 0.0037) number of grade 1-2 blastocysts harvested from the uterine horn ipsilateral to control ovaries in comparison to that collected from the treated ones (6.63 ± 2.92 and 14.75 ± 5.92, respectively) suggests that intra-ovarian injection of PRP before superovulation could exert beneficial effects both in latent follicle growth and in vivo embryo production.

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Section: Introductionmentioning

confidence: 99%

Improvement of Embryo Recovery in Holstein Cows Treated by Intra-Ovarian Platelet Rich Plasma before Superovulation

Crémonesi

Bonfanti

Idda

et al. 2020

Veterinary Sciences

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“…PRP-C produced with the method evaluated in the present study presented a slightly lower concentration of PLTs (947 × 10 3 PLTs/ μ L) when compared with the method described by Lange-Consiglio et al [11, 12] who achieved 1000 × 10 3 PLTs/ μ L. Furthermore, this same hemoderivative presented a lower concentration of PLTs when compared with the technique developed by Tsuzuki et al [13] who achieved 1528 × 10 3 PLTs/ μ L. On the other hand, the other PRP layers (A, B, and D) evaluated in the present study presented lower PLT concentrations in comparison with those obtained in the aforementioned studies [11–13] and even in comparison to the PLT counts in whole blood of the same cows. Unfortunately, the WBC concentration was not reported for the techniques used for producing PRPs in cattle [11, 12, 29], which prevents us from comparing our findings with those reports.…”

Section: Discussionmentioning

confidence: 48%

“…Unfortunately, the WBC concentration was not reported for the techniques used for producing PRPs in cattle [11, 12, 29], which prevents us from comparing our findings with those reports.…”

Section: Discussionmentioning

confidence: 72%

“…Furthermore, there are studies about the use of several PRP-related hemoderivatives for the treatment of mastitis [11], reproductive problems [12], and sole ulcers [13] in cows with encouraging results. The basic biology of PRP (mostly cellular and molecular studies) has been more extensively studied in horses [14–18] and to a lesser degree in dogs [19–21].…”

Section: Introductionmentioning

confidence: 99%

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Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Gutiérrez

López

Giraldo

et al. 2017

Veterinary Medicine International

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There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs.

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“…The study by Anitua et al [12] used PRP that was either rich or poor in leucocytes in order to evaluate whether this could influence tissue healing; however, it appeared that leucocyte concentrations did not interfere with wound healing after oral surgery. Some issues were raised by Del Buono and colleagues [20] against the indiscriminate use of PRP or PRGF.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of platelet-rich or growth factor-rich plasma on intestinal anastomosis healing in pigs

et al. 2017

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BackgroundThe use of autologous platelet-rich plasma (PRP) and plasma rich in growth factors (PRGF) has been proposed for the treatment of several acute and chronic syndromes, such as corneal epithelial defects and dry eye syndrome, gum bleeding during oral surgery, and in orthopaedic surgery. We hypothesized that PRGF, rather than PRP, could be more effective because of its intrinsic characteristics in promoting the healing of intestinal anastomosis. The purpose of the present study was to evaluate and compare the effects of PRP and PRGF on various parameters of anastomotic healing in a swine model.MethodsEight female pigs were randomly assigned to two groups and subjected to hand sewn jeujuno-jejunal appositional extramucosal anastomoses. For each animal, a total of six anastomoses were performed: two were considered controls and received no treatment, while the remaining four anastomoses were treated with PRP or PRGF of which both were prepared at a platelet concentration that was respectively 3.4-fold and 2.81-fold higher than the original platelet count. In each animal, either PRP or PRGF was used as a treatment, to avoid interference among products. Animals were euthanized after 8 days and the anastomoses were evaluated and compared for the presence of adhesions, anastomotic leakage, bursting pressure, and histological appearance.ResultsThe concentration of platelets in PRP was 3.41-fold higher (range, 3.20–4.24) that the concentration in whole blood, while the concentration in PRGF was 2.81-fold higher (range, 2.89–4.88).The results obtained from the present study highlighted that there are no differences between anastomotic samples treated with either PRP or PRGF preparations, except for a significant increase in epithelization of the intestinal mucosa at the anastomotic site in the PRGF group.ConclusionsBoth PRP and PRGF suspensions should be considered a safe strategy and represent a relatively low-cost technology that is flexible enough to be applied in several therapeutic fields. No true benefit could be proven in our study compared to the no treatment following anastomoses formation, with the exception of enhanced epithelization of the mucosa in the PRGF group.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1102-8) contains supplementary material, which is available to authorized users.

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Platelet concentrate in bovine reproduction: effects on in vitro embryo production and after intrauterine administration in repeat breeder cows

Cited by 31 publications

References 44 publications

Improvement of Embryo Recovery in Holstein Cows Treated by Intra-Ovarian Platelet Rich Plasma before Superovulation

Improvement of Embryo Recovery in Holstein Cows Treated by Intra-Ovarian Platelet Rich Plasma before Superovulation

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Comparison of the effects of platelet-rich or growth factor-rich plasma on intestinal anastomosis healing in pigs

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